Modulation of ROS production in human leukocytes by ganglioside micelles

Gavella, Mirjana; Kveder, Marina; Lipovac, Vaskresenija

doi:10.1590/s0100-879x2010007500092

Cited by 13 publications

(8 citation statements)

References 38 publications

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“…One of the suggested processes of spermatozoa defiance to cryostorage-produced damage is its ability to shed hydrophilic lipids, therefore increasing the hydrophobic features of the membrane and increasing spermatozoa tolerance to cryopreservation [68]. Gavella et al [23] showed that treatment of GM1 and GT1b defended human spermatozoa DNA integrity during the freezing-thawing procedures. GM1-pretreated cryopreserved embryonic ventral mesencephalon (VM) plus a daily treatment with GM1 to the culture medium improves the low survival and functional inefficacy of grafts derived from cryopreserved VM in rats [69].…”

Section: Protective Effect Of Gangliosides In Germ Cells and Early Emmentioning

confidence: 99%

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Effects of Gangliosides on Spermatozoa, Oocytes, and Preimplantation Embryos

Kim

et al. 2019

IJMS

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Gangliosides are sialic acid-containing glycosphingolipids, which are the most abundant family of glycolipids in eukaryotes. Gangliosides have been suggested to be important lipid molecules required for the control of cellular procedures, such as cell differentiation, proliferation, and signaling. GD1a is expressed in interstitial cells during ovarian maturation in mice and exogenous GD1a is important to oocyte maturation, monospermic fertilization, and embryonic development. In this context, GM1 is known to influence signaling pathways in cells and is important in sperm–oocyte interactions and sperm maturation processes, such as capacitation. GM3 is expressed in the vertebrate oocyte cytoplasm, and exogenously added GM3 induces apoptosis and DNA injury during in vitro oocyte maturation and embryogenesis. As a consequence of this, ganglioside GT1b and GM1 decrease DNA fragmentation and act as H2O2 inhibitors on germ cells and preimplantation embryos. This review describes the functional roles of gangliosides in spermatozoa, oocytes, and early embryonic development.

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Section: Protective Effect Of Gangliosides In Germ Cells and Early Emmentioning

confidence: 99%

“…For example, treatment with GM1 protects against oxidative stress-induced neuronal cell injury and traumatic injury-induced brain edema [22]. Supplementation of GT1b to culture medium also protects human spermatozoa from DNA damage induced by ROS [23].…”

Section: Introductionmentioning

confidence: 99%

Effects of Gangliosides on Spermatozoa, Oocytes, and Preimplantation Embryos

Kim

et al. 2019

IJMS

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“…The observed effect can be attributed to the ability of ganglioside micelles attached to the cell surface to provide a diffusion barrier and consequently delay membrane events responsible for superoxide anion production ( Figure 3). 64 The presence of leukocytes on the sperm surface and the similarity of their cellular mechanisms for superoxide generation to those of activated spermatozoa could explain the decrease in superoxide anion generation in spermatozoa. 65,66 It is believed that leukocytes and spermatozoa share a similar mechanism for ROS generation: an NADPH oxidase located in or near the cell membrane.…”

Section: Gangliosides and Spermatozoamentioning

confidence: 99%

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Gavella¹,

Lipovac²

2013

Asian J Androl

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This article summarizes the available evidence on the efficacy of gangliosides to reduce the degree of reactive oxygen species (ROS)-mediated damage. The antioxidative efficacy of exogenous gangliosides in protecting different cells encouraged us to examine their ability to protect human spermatozoa. Gangliosides are sialic acid-containing glycosphingolipids with strong amphiphilic character due to the bulky headgroup made of several sugar rings with sialic acid residues and the double-tailed hydrophobic lipid moiety. The amphiphilicity of gangliosides allows them to exist as micelles in aqueous media when they are present at a concentration above their critical micellar concentration. The protective effect of ganglioside micelles on spermatozoa is believed to stem from their ability to scavenge free radicals and prevent their damaging effects. In our study, we particularly focused our attention on the protective effect of ganglioside micelles on DNA in human spermatozoa exposed to cryopreservation. The results indicate that ganglioside micelles can modulate the hydrophobic properties of the sperm membrane to increase tolerance to DNA fragmentation, thus protecting the DNA from cryopreservation-induced damage. Further actions of ganglioside micelles, which were documented by biochemical and biophysical studies, included (i) the modulation of superoxide anion generation by increasing the diffusion barrier for membrane events responsible for signal translocation to the interior of the cell; (ii) the inhibition of iron-catalysed hydroxyl radical formation due to the iron chelation potential of gangliosides; and (iii) inhibition of hydrogen peroxide diffusion across the sperm membrane.

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“…There is also evidence that caveolin-1, an important component of caveolae, interacts with Hmox1, modulates its activity, and can act as a natural competitive inhibitor of Hmox1 with heme [ 17 ]. Moreover, gangliosides have been found to inhibit hydroxyl radical formation in vitro [ 18 ] and also modulate ROS formation in human leukocytes [ 19 ] and neuronal cells [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Heme Oxygenase-1 May Affect Cell Signalling via Modulation of Ganglioside Composition

Šmíd

Šuk

Kachamakova‐Trojanowska

et al. 2018

Oxidative Medicine and Cellular Longevity

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Heme oxygenase 1 (Hmox1), a ubiquitous enzyme degrading heme to carbon monoxide, iron, and biliverdin, is one of the cytoprotective enzymes induced in response to a variety of stimuli, including cellular oxidative stress. Gangliosides, sialic acid-containing glycosphingolipids expressed in all cells, are involved in cell recognition, signalling, and membrane stabilization. Their expression is often altered under many pathological and physiological conditions including cell death, proliferation, and differentiation. The aim of this study was to assess the possible role of Hmox1 in ganglioside metabolism in relation to oxidative stress. The content of liver and brain gangliosides, their cellular distribution, and mRNA as well as protein expression of key glycosyltransferases were determined in Hmox1 knockout mice as well as their wild-type littermates. To elucidate the possible underlying mechanisms between Hmox1 and ganglioside metabolism, hepatoblastoma HepG2 and neuroblastoma SH-SY5Y cell lines were used for in vitro experiments. Mice lacking Hmox1 exhibited a significant increase in concentrations of liver and brain gangliosides and in mRNA expression of the key enzymes of ganglioside metabolism. A marked shift of GM1 ganglioside from the subsinusoidal part of the intracellular compartment into sinusoidal membranes of hepatocytes was shown in Hmox1 knockout mice. Induction of oxidative stress by chenodeoxycholic acid in vitro resulted in a significant increase in GM3, GM2, and GD1a gangliosides in SH-SY5Y cells and GM3 and GM2 in the HepG2 cell line. These changes were abolished with administration of bilirubin, a potent antioxidant agent. These observations were closely related to oxidative stress-mediated changes in sialyltransferase expression regulated at least partially through the protein kinase C pathway. We conclude that oxidative stress is an important factor modulating synthesis and distribution of gangliosides in vivo and in vitro which might affect ganglioside signalling in higher organisms.

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Modulation of ROS production in human leukocytes by ganglioside micelles

Cited by 13 publications

References 38 publications

Effects of Gangliosides on Spermatozoa, Oocytes, and Preimplantation Embryos

Effects of Gangliosides on Spermatozoa, Oocytes, and Preimplantation Embryos

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Heme Oxygenase-1 May Affect Cell Signalling via Modulation of Ganglioside Composition

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