Effects of Gangliosides on Spermatozoa, Oocytes, and Preimplantation Embryos

Kim, Bo Hyun; Ju, Won Seok; Kim, Jisu; Park, Soon Ju; Ward, Sean M.; Lyu, Ju Hyeong; Choo, Young‐Kug

doi:10.3390/ijms21010106

Cited by 13 publications

(21 citation statements)

References 112 publications

(124 reference statements)

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“…The HEXA gene in our study was also significantly higher expressed in porcine blastocysts which corresponds to upregulation of a substrate specific cofactor GM2A. Most of publications describe GM1, GM3 and GD1a gangliosides as important factors involved in the EGFR (epidermal growth factor receptor) signaling pathway [28]. In porcine embryos, exogenous GD1a supported maturation and blastocyst formation in vitro [29].…”

Section: Discussionsupporting

confidence: 62%

“…GM2 Activator, also known as SAP3 (Shingolipid Activator Protein 3), coded by the GM2A gene, is one of the components of the B-hexosaminidaseA (HEXA) complex necessary for the enzymatic processing of gangliosides, which are sialic acid-containing acidic glycosphingolipids. Gangliosides found in animal plasma membranes exhibit cell-type specificity and are implicated in various cellular functions, differentiation, signal transduction and immunity [27,28]. The HEXA gene in our study was also significantly higher expressed in porcine blastocysts which corresponds to upregulation of a substrate specific cofactor GM2A.…”

Section: Discussionsupporting

confidence: 56%

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Lipid Stores and Lipid Metabolism Associated Gene Expression in Porcine and Bovine Parthenogenetic Embryos Revealed by Fluorescent Staining and RNA-seq

Kajdasz

Warzych

Derebecka

et al. 2020

IJMS

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Compared to other mammalian species, porcine oocytes and embryos are characterized by large amounts of lipids stored mainly in the form of droplets in the cytoplasm. The amount and the morphology of lipid droplets (LD) change throughout the preimplantation development, however, relatively little is known about expression of genes involved in lipid metabolism of early embryos. We compared porcine and bovine blastocyst stage embryos as well as dissected inner cell mass (ICM) and trophoblast (TE) cell populations with regard to lipid droplet storage and expression of genes functionally annotated to selected lipid gene ontology terms using RNA-seq. Comparing the number and the volume occupied by LD between bovine and porcine blastocysts, we have found significant differences both at the level of single embryo and a single blastomere. Aside from different lipid content, we found that embryos regulate the lipid metabolism differentially at the gene expression level. Out of 125 genes, we found 73 to be differentially expressed between entire porcine and bovine blastocyst, and 36 and 51 to be divergent between ICM and TE cell lines. We noticed significant involvement of cholesterol and ganglioside metabolism in preimplantation embryos, as well as a possible shift towards glucose, rather than pyruvate dependence in bovine embryos. A number of genes like DGAT1, CD36 or NR1H3 may serve as lipid associated markers indicating distinct regulatory mechanisms, while upregulated PLIN2, APOA1, SOAT1 indicate significant function during blastocyst formation and cell differentiation in both models.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionsupporting

confidence: 56%

Lipid Stores and Lipid Metabolism Associated Gene Expression in Porcine and Bovine Parthenogenetic Embryos Revealed by Fluorescent Staining and RNA-seq

Kajdasz

Warzych

Derebecka

et al. 2020

IJMS

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“…Because the reduction of average litter size was obvious in F3, our further analysis focused on the comparison between F3 and 3M groups. It has been shown that decrease in litter size could result from dysfunctional uteri or decreasing embryo number [20,21], and most problems during embryonic development are associated with placentation defects [22]. In examining day 12 (D12) pregnant mice, F3 mice were revealed to have abnormal uteri compared with 3M mice (Fig.…”

Section: Pregnancy Outcome and Placental Developmentmentioning

confidence: 99%

Female Reproductive Failures in Mouse Adolescent Pregnancy

Yang¹,

Y²,

Pan³

et al. 2021

Preprint

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Background: There are around 300 million adolescent pregnancies worldwide, accounting for 11% of all births worldwide. Accumulating evidence demonstrates that many adverse perinatal outcomes are associated with adolescent pregnancies. However, how and why these abnormalities occur remain to be defined. Methods: To compare uterine maturity, implantation, decidualization and placental development between 25-30 days old and 3 months old mature female CD-1 strain mice. Both in vivo mouse pregnancy and in vitro cell culture were used. Western blot, real time RT-PCR, immunostaining and immunofluorescence were used to study the progress of pregnancy during two groups.Results: We found the litter size of adolescent pregnancy is significantly decreased from F1 to F3 generations compared to mature pregnancy. On days 8 and 12 of pregnancy, multiple abnormalities in placental and decidual developments appear in F3 adolescent pregnancy. On days 5 and 8, uterine endoplasmic reticulum stress is dysregulated compared to mature pregnancy. Embryo implantation and decidualization are also compromised in adolescent pregnancy. Many genes are abnormally expressed in adolescent estrous uteri. Conclusion: The uterine immaturities and abnormal implantation may cause multiple pregnancy failures in adolescent pregnancy.

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“…This effect of EBN might be due to its potential bioactive components; the nutritional composition of EBN is protein, carbohydrate, ash, and lipids (Ma & Liu, 2012), while sialic acid is the major carbohydrate component found in EBN (Daud et al, 2019). Sialic acid-containing gangliosides have been demonstrated to efficiently protect oocytes and embryos from ROS produced injury, which is one of the major mechanisms of Cd toxicity (Kim et al, 2020;Wang et al, 2019).…”

Section: Histopathological Examinationmentioning

confidence: 99%

Protective Effect of Edible Bird’s Nest on Ovaries of Cycling Female Rats Subjected to Cadmium Toxicity

Quddus¹,

Yimer²,

Jesse³

et al. 2021

JAHP

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| Cadmium (Cd), a toxic heavy metal, is often associated with the reproductive disorders of mammals. The edible bird's nest (EBN) is a naturally occurring food product made from the saliva of swiftlet birds (Aerodramus fuciphagus & Aerodramus maximus) in the form of a nest, and it has been consumed as healthy food or tonic for decades. This research aimed to study the possible protective effects of EBN against Cd toxicity in ovaries of Sprague Dawley rats. Thirty (30) female Sprague Dawley rats were assigned into five groups as follows: group 1-Negative control (NC) received distilled water; group 2-positive control (PC) administered with CdCl 2 , 5mg/kg BW; while groups EBN 1, EBN 2, and EBN 3 received CdCl 2 (5mg/kg BW) concurrently with graded concentrations of 60, 90 and 120 mg/kg BW of EBN, respectively. After four weeks of treatment, rats were euthanized to collect ovaries for histopathological studies using hematoxylin and eosin (H&E) stain. Expressions of vascular endothelial growth factor (VEGF) were analyzed through immunohistochemistry, and levels of superoxidase dismutase (SOD) were assessed using a SOD assay kit. Oral administration of CdCl 2 without EBN supplement resulted in significantly decreased (p<0.05) immunohistochemical expressions of VEGF in ovarian tissue and a decrease (p<0.05) in the activity of SOD. Moreover, ovarian histopathological changes, including follicular cysts and a significant increase (p<0.05) in the number of atretic follicles, while a decrease in the number of growing follicles was noted in Cd only treated group. Animals treated with CdCl 2 and EBN at three different dosages resulted in significantly increased (p<0.05) expressions of VEGF in ovarian tissues, low degenerative changes with normal histomorphology as well as significantly increased (p<0.05) SOD activity as compared to the PC group. Overall, the findings revealed that oral exposure to Cd at a dose rate of 5 mg/kg BW resulted in significant alterations in ovaries, as evidenced by a lower degree of VEGF expression along with reduced antioxidant activity and histomorphological changes. Meanwhile, EBN proved to exhibit a significant protective role against Cd toxicity in ovaries, possibly through its antioxidant effect.

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Effects of Gangliosides on Spermatozoa, Oocytes, and Preimplantation Embryos

Cited by 13 publications

References 112 publications

Lipid Stores and Lipid Metabolism Associated Gene Expression in Porcine and Bovine Parthenogenetic Embryos Revealed by Fluorescent Staining and RNA-seq

Lipid Stores and Lipid Metabolism Associated Gene Expression in Porcine and Bovine Parthenogenetic Embryos Revealed by Fluorescent Staining and RNA-seq

Female Reproductive Failures in Mouse Adolescent Pregnancy

Protective Effect of Edible Bird’s Nest on Ovaries of Cycling Female Rats Subjected to Cadmium Toxicity

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