A low concentration of ouabain (0.18 µg/kg) enhances hypertension in spontaneously hypertensive rats by inhibiting the Na+ pump and activating the renin-angiotensin system

Siman, Fabiana Dayse Magalhães; Stefanon, Ivanita; Vassallo, Dalton Valentim; Padilha, Alessandra Simão

doi:10.1590/s0100-879x2010007500061

Cited by 7 publications

(12 citation statements)

References 31 publications

(63 reference statements)

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“…On the other hand, the central mechanism described above suggests that ouabain stimulates the brain renin-angiotensin system, leading to the production of angiotensin II, which further increases sympathetic tone and ultimately increases arterial blood pressure (47). We also found that the increases in systolic and diastolic blood pressure induced by low-dose ouabain (~0.3 nmol/kg) were blocked completely by pretreatment (iv) with 10 mg/kg losartan, although these increases were not affected by ganglionic blockade with 5 mg/kg hexamethonium, suggesting that ouabain, at least at this concentration, does not act on preganglionic neurons (28,31). On the other hand, the increase of blood pressure induced by 0.18 µg/kg ouabain in L-NAME-hypertensive rats is blocked by hexamethonium (29).…”

Section: Acute Effects Of Picomole Quantities Of Ouabain On Vascular mentioning

confidence: 59%

“…In some rat models of hypertension, including the SHR model (28), renovascular hypertension and L-NAMEinduced hypertension (29), acute ouabain administration increases blood pressure, whereas no change in blood pressure is observed in normotensive rats (14,30,31). These results suggest that the effects of ouabain are amplified in hypertensive conditions.…”

Section: Acute Effects Of Picomole Quantities Of Ouabain On Vascular mentioning

confidence: 96%

“…Acutely, ouabain in picomole quantities increases blood pressure (24,(28)(29)(30)(31), pressor and vascular reactivity to vasoconstrictor agents (14,24,(28)(29)(30)(31)40,41), and sympathoexcitatory activity (9,53). These effects of ouabain are due at least in part to activation of the central and peripheral renin-angiotensin system (31,28,(50)(51)(52).…”

Section: General Considerationsmentioning

confidence: 99%

“…These effects of ouabain are due at least in part to activation of the central and peripheral renin-angiotensin system (31,28,(50)(51)(52).…”

Section: General Considerationsmentioning

confidence: 99%

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Hypertensive effects of the iv administration of picomoles of ouabain

Padilha

Salaíces

Vassallo

et al. 2011

Braz J Med Biol Res

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Ouabain, an endogenous digitalis compound, has been detected in nanomolar concentrations in the plasma of several mammals and is associated with the development of hypertension. In addition, plasma ouabain is increased in several hypertension models, and the acute or chronic administration of ouabain increases blood pressure in rodents. These results suggest a possible association between ouabain and the genesis or development and maintenance of arterial hypertension. One explanation for this association is that ouabain binds to the α-subunit of the Na + pump, inhibiting its activity. Inhibition of this pump increases intracellular Na + , which reduces the activity of the sarcolemmal Na + /Ca 2+ exchanger and thereby reduces Ca 2+ extrusion. Consequently, intracellular Ca 2+ increases and is taken up by the sarcoplasmic reticulum, which, upon activation, releases more calcium and increases the vascular smooth muscle tone. In fact, acute treatment with ouabain enhances the vascular reactivity to vasopressor agents, increases the release of norepinephrine from the perivascular adrenergic nerve endings and promotes increases in the activity of endothelial angiotensin-converting enzyme and the local synthesis of angiotensin II in the tail vascular bed. Additionally, the hypertension induced by ouabain has been associated with central mechanisms that increase sympathetic tone, subsequent to the activation of the cerebral renin-angiotensin system. Thus, the association with peripheral mechanisms and central mechanisms, mainly involving the renin-angiotensin system, may contribute to the acute effects of ouabain-induced elevation of arterial blood pressure.

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Section: Acute Effects Of Picomole Quantities Of Ouabain On Vascular mentioning

confidence: 59%

Section: Acute Effects Of Picomole Quantities Of Ouabain On Vascular mentioning

confidence: 96%

Section: General Considerationsmentioning

confidence: 99%

“…These effects of ouabain are due at least in part to activation of the central and peripheral renin-angiotensin system (31,28,(50)(51)(52).…”

Section: General Considerationsmentioning

confidence: 99%

See 2 more Smart Citations

Hypertensive effects of the iv administration of picomoles of ouabain

Padilha

Salaíces

Vassallo

et al. 2011

Braz J Med Biol Res

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“…The relative participation of the SR is more important for post-rest contractions than for steady state contractions. Pause intervals of various durations (15,30 and 60 s) were tested and the results were presented as the relative potentiation (the amplitude of post-rest contractions divided by steady-state contractions) to normalize the data from different preparations. 4.…”

Section: Isolated Papillary Musclesmentioning

confidence: 99%

Low-dose ouabain administration increases Na+,K+-ATPase activity and reduces cardiac force development in rats

Meira

Siman

Faria

et al. 2015

Pharmacological Reports

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Maternal protein restriction compromises myocardial contractility in the young adult rat by changing proteins involved in calcium handling

Belchior

Freire

Costa

et al. 2016

Journal of Applied Physiology

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Maternal protein restriction (MPR) during pregnancy is associated with increased cardiovascular risk in the offspring in adulthood. In this study we evaluated the cardiac function of young male rats born from mothers subjected to MPR during pregnancy, focusing on the myocardial mechanics and calcium-handling proteins. After weaning, rats received normal diet until 3 mo old, when the following parameters were assessed: arterial and left ventricular hemodynamics and in vitro cardiac contractility in isolated papillary muscles. The body weight was lower and arterial pressure higher in the MPR group compared with young adult offspring of female rats that received standard diet (controls); and left ventricle time derivatives increased in the MPR group. The force developed by the cardiac muscle was similar; but time to peak and relaxation time were longer, and the derivatives of force were depressed in the MPR. In addition, MPR group exhibited decreased post-pause potentiation of force, suggesting reduced reuptake function of the sarcoplasmic reticulum. Corroborating, the myocardial content of SERCA-2a and phosphorylated PLB-Ser16/total PLB ratio was decreased and sodium-calcium exchanger was increased in the MPR group. The contraction dependent on transsarcolemmal influx of calcium was higher in MPR if compared with the control group. In summary, young rats born from mothers subjected to protein restriction during pregnancy exhibit changes in the myocardial mechanics with altered expression of calcium-handling proteins, reinforcing the hypothesis that maternal malnutrition is related to increased cardiovascular risk in the offspring, not only for hypertension, but also cardiac dysfunction.

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A low concentration of ouabain (0.18 µg/kg) enhances hypertension in spontaneously hypertensive rats by inhibiting the Na+ pump and activating the renin-angiotensin system

Cited by 7 publications

References 31 publications

Hypertensive effects of the iv administration of picomoles of ouabain

Hypertensive effects of the iv administration of picomoles of ouabain

Low-dose ouabain administration increases Na+,K+-ATPase activity and reduces cardiac force development in rats

Maternal protein restriction compromises myocardial contractility in the young adult rat by changing proteins involved in calcium handling

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