Low-dose ouabain administration increases Na+,K+-ATPase activity and reduces cardiac force development in rats

Meira, Eduardo Frizzera; Siman, Fabiana Dayse Magalhães; Faria, Thaís de Oliveira; Júnior, Rogério Faustino Ribeiro; Batista, Priscila Rossi de; Stefanon, Ivanita; Vassallo, Dalton Valentim; Padilha, Alessandra Simão

doi:10.1016/j.pharep.2014.10.005

Cited by 7 publications

(2 citation statements)

References 38 publications

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“…Intestinal samples were homogenized in ten volumes (w/v) of ice‐cold physiological saline on ice and then centrifuged at 2,500 g for 10 min at 4°C; the supernatant was stored at 4°C as the crude extract, and then, all assays were conducted within 24 hr. After the centrifugation, the supernatant was used for the determination of trypsin (Erlanger, Kokowsky, & Cohen, ), lipase (Furne et al., ), amylase (Robyt & Whelan, ) and Na + , K + ‐ATPase (Meira et al., ) activities. Alkaline phosphatase activity was measured according to the method of Bessey (Bessey, Lowry, & Brock, ).…”

Section: Methodsmentioning

confidence: 99%

Dietary available phosphorus requirement for on-growing gibel carp (Carassius auratus gibeliovar. CAS III)

et al. 2017

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A nine-week feeding experiment was conducted in flow-through system with gibel carp (43.8 ± 0.2 g) to study the effects of dietary available phosphorus (P) on growth, phosphorous digestibility and intestinal enzyme activities. Seven semipurified diets were formulated to contain 0.8 (the basal), 2.4, 3.6, 6.1, 7.4, 10.1 and 15.8 g available phosphorus kg −1 diet. The results showed that specific growth rate and feed efficiency increased with increasing dietary available P from 0.8 to 7.4 g P kg −1 . Fish body ash increased with increasing dietary available P, while moisture, protein content or energy content had no difference. Total phosphorus waste discharging (TPW) increased with increased dietary phosphorous. Plasma glucose was higher in the fish fed with 7.4 g kg −1 P. Plasma triglycerides was lower in fish fed diets containing 6.1-10.1 g kg −1 P. No significant effects were observed in plasma P and Ca (p > .05). The activities of intestinal amylase, lipase and trypsin showed no difference, while AKP and Na + , K + -ATPase activities decreased with increasing dietary available P. In conclusion, based on the regression between specific growth rate (SGR), P retention efficiency, feed efficiency (FE) and dietary available P, the available P requirements for on-growing gibel carp were 10.69, 8.22 and 6.72 g kg −1 , respectively. in many fish species. In order to acquire enough P, fish have to obtain from the diet because fresh water and sea water are low in P concentration (Boyd, 1971). Phosphorus concentrations in most commercial diets considerably exceed the estimated requirements (Rodehutscord & Pfeffer, 1995). Phosphorus is the first limiting factor for algal growth in fresh water, and the excess phosphorus excreted from cultured animals into water can stimulate eutrophication of water bodies and result in deteriorating water quality (Kim, Kim, Song, Lee, & Jeong, 1998). So it is essential to precisely determine the dietary P requirement in order to minimize excess P without risking P deficiency in cultured fish. K E Y W O R D SThere have been many studies regarding the P requirements for different fishes; the dietary phosphorus requirements for fish species have been reported to range from 3.0 to 15.0 g kg -1 of diet (NRC, 2011). But most studies were focusing on small fish and there is few detailed information about available phosphorus requirements for on-growing fish. Studies have been reported, 5.8 g kg -1 available P for juvenile catfish (Clarias leather) (Yu et al., 2013), 3.0 g kg -1for on-growing catfish (Ictalurus punctatus) (Eya & Lovell, 1997), no information on the available phosphorus requirement for on-growing gibel carp. Therefore, the present study was designed to determine the dietary available phosphorus requirement for on-growing gibel carp and to investigate the effect of dietary P levels on growth performance, body composition, P bioaccumulation and the intestinal enzyme activities.| 1105 XIE et al. 9.5-10.0 g kg -1 available P for juvenile tilapia (Oreochromis niloticus fe...

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Section: Methodsmentioning

confidence: 99%

Dietary available phosphorus requirement for on-growing gibel carp (Carassius auratus gibeliovar. CAS III)

et al. 2017

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“…Branchial, Renal and Intestine samples were homogenized in ten volumes (w/v) of ice‐cold physiological saline on ice and then centrifuged at 2,500 g for 10 min at 4°C, the supernatant was stored at 4°C as the crude extract and then all assays were conducted within 24 hr. After centrifugation, the supernatant were used for the determination of trypsin (Erlanger, Kokowsky, & Cohen, ), lipase (Furné et al., ), amylase (Robyt & Whelan, ), Na + /K + ‐ATPase (Meira et al., ) and Ca 2+ ‐ATPase (Niggli, Adunyah, Penniston, & Carafoli, ). Plasma alkaline phosphatase activity was measured according to Bessey (Bessey, Lowry, & Brock, ).…”

Section: Methodsmentioning

confidence: 99%

Dietary available phosphorus requirement for juvenile gibel carp (Carassius auratus gibeliovar. CASIII)

et al. 2017

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A 57-day growth experiment was conducted with juvenile gibel carp (13.48 AE 0.10 g) in a flow-through system to study the effect of dietary phosphorus on growth performance, body composition, nutrition utilization, phosphorus loading and enzymes activities. Seven semipurifed diets were formulated to contain 0.07 (the basal), 2. 27, 5.32, 8.10, 12.06, 15.24 and 19.48 g available phosphorus/kg diet.The results showed that specific growth rate, body length and feed efficiency significantly increased in the fish fed diets containing 0.07 to 15.24 g available P/kg diet (p < .05). Ash and P content increased in fish fed diets containing 0.07-12.06 P g/kg (p < .05) and then levelled off, while moisture, crude protein and lipid had no significant difference (p > .05). The protein retention efficiency increased in the fish fed with diets 0.07-5.32 g/kg P (p < .05) and then reached a plateau. The P content in faeces was higher in fish fed diets containing 15.24 and 19.48 g available P/kg. Total P concentration in tank water increased in fish fed 0.07-12.06 g available P per kg diet (p < .05). The plasma P was higher in the fish fed with 15.24 g available P/kg diet (p < .05), triglycerides was lower in the fish fed diet containing 15.24 and 19.48 g available P/kg (p < .05), no significant differences were observed in plasma Ca, plasma glucose and calcitonin (p > .05). Based on SGR, whole body P content and FE, dietary available P requirement for juvenile gibel carp were 13.37, 13.97 g/kg and 15.06 respectively. K E Y W O R D S growth performance, juvenile gibel carp, nutrition utilization, P loading, phosphorus wileyonlinelibrary.com/journal/are Aquaculture Research. 2018;49:1284-1292.

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Low dose ouabain stimulates Na K ATPase α1 subunit association with angiotensin II type 1 receptor in renal proximal tubule cells

Ketchem

Conner

Murray

et al. 2016

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Our laboratory has recently demonstrated that low concentrations of ouabain increase blood pressure in rats associated with stimulation of Na-K ATPase activity and activation of the Src signaling cascade in NHE1-dependent manner. Proteomic analysis of human kidney proximal tubule cells (HKC11) suggested that the Angiotensin II type 1 receptor (AT1R) as an ouabain-associating protein. We hypothesize that ouabain-induced stimulation of Na-K ATPase activity is mediated through AT1R. To test this hypothesis, we examined the effect of ouabain on renal cell angiotensin II production, the effect of AT1R inhibition on ouabain-stimulated NKA activity, and the effect of ouabain on NKA-AT1R association. Ouabain increased plasma angiotensin II levels in rats treated with ouabain (1 μg/kg body wt./day) for 9 days and increased angiotensin II levels in cell culture media after 24 h treatment with ouabain in human (HKC11), mouse (MRPT), canine (MDCK) kidney cells, and human adrenal cells. Ouabain 10 pM stimulated NKA-mediated 86Rb uptake and phosphorylation of EGFR, Src, and ERK1/2. These effects were prevented by the AT1R receptor blocker candesartan. FRET and TIRF microscopy using Bodipy-labeled ouabain and mCherry-NKA or mCherry-AT1R demonstrated association of ouabain with AT1R and NKA. Further our FRET and TIRF studies demonstrated increased association between AT1R and NKA upon treatment with low dose ouabain. We conclude that ouabain stimulates NKA in renal proximal tubule cells through an angiotensin/AT1R-dependent mechanism and that this pathway contributes to cardiac glycoside associated hypertension.

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Low-dose ouabain administration increases Na+,K+-ATPase activity and reduces cardiac force development in rats

Abstract: We suggest that the effects induced by ouabain in the isolated cardiac muscle could be related at least in part, to changes in NKA activity.

Cited by 7 publications

References 38 publications

Dietary available phosphorus requirement for on-growing gibel carp (Carassius auratus gibeliovar. CAS III)

Dietary available phosphorus requirement for on-growing gibel carp (Carassius auratus gibeliovar. CAS III)

Dietary available phosphorus requirement for juvenile gibel carp (Carassius auratus gibeliovar. CASIII)

Low dose ouabain stimulates Na K ATPase α1 subunit association with angiotensin II type 1 receptor in renal proximal tubule cells

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