Effect of hyperbaric oxygenation on the regeneration of the liver after partial hepatectomy in rats

Abstract

The aim of the present study was to assess the influence of hyperbaric oxygenation (HBO) on rat liver regeneration before and after partial hepatectomy. Rats were sacrificed 54 h after 15% hepatectomy, liver and body weights were measured, and serum alanine transaminase (ALT) and aspartate transaminase (AST) activity and albumin levels were determined. The lipid peroxide level, as indicated by malondialdehyde production in the remnant liver was measured, and liver sections were analyzed by light microscopy. Five groups of 10 rats in each group were studied. The preHBO and pre-hyperbaric pressure (preHB) groups were treated before partial hepatectomy with 100% O 2 and 21% O 2 , respectively, at 202,650 pascals, daily for 3 days (45 min/ day). The control group was not treated before partial hepatectomy and recovered under normal ambient conditions after the procedure. Groups postHBO and postHB were treated after partial hepatectomy with HBO and HB, respectively, three times (45 min/day). The preHBO group presented a significant increase in the initiation of the regeneration process of the liver 54 h postoperatively. The liver/body weight ratio was 0.0618 ± 0.0084 in the preHBO compared to 0.0517 ± 0016 g/g in the control animals (P = 0.016). In addition, the preHBO group showed significant better liver function (evaluated by the lowest serum ALT and AST activities, P = 0.002 and P = 0.008, respectively) and showed a significant decrease in serum albumin levels compared to control (P < 0.001). Liver lipid peroxide concentration was lowest in the preHBO group (P < 0.001 vs control and postHBO group) and light microscopy revealed that the composition of liver lobules in the preHBO group was the closest to normal histological features. These results suggest that HBO pretreatment was beneficial for rat liver regeneration after partial hepatectomy.

Farelo integral de arroz parboilizado submetido a armazenamento prolongado para alimentação de codornas de corte

“…O alto nível de peróxidos na gordura hepática e a presença de muitos dos compostos tóxicos formados durante a oxidação podem provocar danos às células epiteliais do fígado. Além disso, mudanças severas na composição normal de ácidos graxos do fígado causam transtornos no metabolismo dos lipídeos, que resultam em alterações no tamanho deste órgão (Kurir et al, 2004). Desse modo, a ausência de variação no peso relativo do fígado das aves alimentadas com rações contendo FIAPA é um indicativo de que os produtos secundários e terciários formados no decorrer do processo oxidativo desse farelo não foram suficientes para produzir mudanças no tamanho desse órgão (Tabela 5).…”

The Effect of Chronic Iron Losses on Liver Regeneration in Male and Female Rats

“…The positive influence of preconditioning on PH has been proved by several authors: by abdominal laparotomy 19 , by periodic ischemia and liver reperfusion 20 , by hyperbaric oxygenation 21 . Repeated blood withdrawals stimulate metabolic iron turnover and partly evoked findings in vitro 22 .…”

Hepatic Regeneration and Reno-Protection by Fish oil, <i>Nigella sativa</i> Oil and Combined Fish Oil/<i>Nigella sativa</i> Volatiles in CCl₄ Treated Rats

“…This occurs due to the hyperplasia of the residual lobes and mitosis of the hepatocytes which are quiescent under normal conditions 29,30 . Some studies have speculated that slight genesis and/or formation of low level of free radicals are important for stimulating liver regeneration and necessary for their natural course 31 . These speculations seem to be only coincided on low concentration of reactive oxygen species because logically elevated oxidative stress could contribute to liver damage.…”