Characterization of bovine respiratory syncytial virus isolated in Brazil

Abstract

This paper presents the first isolation of bovine respiratory syncytial virus in Brazil and its physicochemical, morphological and molecular characterization. The virus was isolated from 33 samples of nasotracheal secretions, successively inoculated into a Madin-Darby bovine kidney cell culture, which was characterized by physicochemical tests and morphological observation by electron microscopy. The Brazilian sample is an RNA pleomorphic, enveloped, thermolabile and nonhemagglutinating spicular virus. Reverse transcription, followed by nested polymerase chain reaction (nRT-PCR) assay was carried out using oligonucleotides B1, B2A, B3 and B4 for the fusion proteins (F) and B5A, B6A, B7A and B8 for the attachment protein (G). The nRT-PCR-F amplified a fragment of 481 bp corresponding to part of the gene that codes for protein F, whereas nRT-PCR-G amplified a fragment of 371 bp, in agreement with part of the G gene. The virus isolated from Brazilian samples in this study corresponded to the bovine respiratory syncytial virus, and RT-PCR proved to be useful for the diagnosis of bovine clinical samples.

Role of Human β-Defensin-2 during Tumor Necrosis Factor-α/NF-κB-mediated Innate Antiviral Response against Human Respiratory Syncytial Virus

“…The mixture was incubated at 37°C for 8 h, followed by the addition of an equal volume of 3% glutaraldehyde. The samples were stored at 4°C until transmission electron microscopy (23,24) was performed with Phillip's CM-10 (University of Texas Health Science Center, San Antonio, Electron Microscope Core Facility).…”

Detection of bovine respiratory syncytial virus in experimentally infected balb/c mice

“…The Brazilian BRSV-25-BR strain, isolated in the Laboratorio de Virologia Animal do Institute de Biologia da Universidade Estadual de Campinas (UNICAMP) [2], was used in the present study. This strain was isolated from samples of nasotracheal secretions collected from live animals from the Southern and Southeastern Brazilian States, inoculated successively in MDBK (Madin Darby Bovine Kidney) and CER (Chicken Embryo Related) cells.…”

Detection of an untyped strain of bovine respiratory syncytial virus in a dairy herd

“…Phylogenetic relatedness of the BRSV-UnespJab-1 strain with other F protein gene sequences is shown in Figure 2. In the dendrogram, the BRSV-UnespJab-1 strain did not group with any of BRSV subgroups proposed by Valarcher, Schelcher and Bourhy (2000), even to subgroup B, in which the BRSV-25-BR isolate, also from Brazil, is classified (ARNS et al, 2003). Therefore, the strain was classified as untyped.…”

“…The subgroup A and AB were found in European countries, United States and Japan (FURZE et al, 1994;STINE;HOPPE;CLAYTON, 1997;LARSEN;TJORNEHOJ;VIUFF, 2000;NETTLETON et al, 2003;YAEGASHI et al, 2005) while the subgroup B was isolated in United Kingdom in the 1970s and later was rediscovered in Brazil (NETTLETON et al, 2003;ALMEIDA et al, 2006;VALARCHER;. All the known Brazilian isolates are classified in this subgroup (ARNS et al, 2003;ALMEIDA et al, 2006;SPILKI et al, 2006;ARNS, 2008;ARNS, 2011). Even though, the occurrence of other subgroups in Brazil may not be disregarded, as the number of BRSV isolates characterized until now is very small.…”