Six years of treatment with the HELP system of a patient with familial hypercholesterolemia

Nascimento, Marcelo Mazza do; Pasqual, D.D.; Santos, José Ernesto dos; Riella, Miguel C.

doi:10.1590/s0100-879x2002000700003

Cited by 2 publications

(1 citation statement)

References 19 publications

(14 reference statements)

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“…High cholesterol levels are a risk factor for arteriosclerosis and hypertension (1,2). In addition to diet, there are two endogenous sources of cholesterol: biliary cholesterol, which is always unesterified, and intestinal epithelial sloughing.…”

Section: Introductionmentioning

confidence: 99%

Cholesterol induces fetal rat enterocyte death in culture

Gazzola

Silva

Kanunfre

et al. 2004

Braz J Med Biol Res

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The effect of cholesterol on fetal rat enterocytes and IEC-6 cells (line originated from normal rat small intestine) was examined. Both cells were cultured in the presence of 20 to 80 µM cholesterol for up to 72 h. Apoptosis was determined by flow cytometric analysis and fluorescence microscopy. The expression of HMG-CoA reductase and peroxisome proliferator-activated receptor gamma (PPARγ) was measured by RT-PCR. The addition of 20 µM cholesterol reduced enterocyte proliferation as early as 6 h of culture. Reduction of enterocyte proliferation by 28 and 41% was observed after 24 h of culture in the presence and absence of 10% fetal calf serum, respectively, with the effect lasting up to 72 h. Treatment of IEC-6 cells with cholesterol for 24 h raised the proportion of cells with fragmented DNA by 9.7% at 40 µM and by 20.8% at 80 µM. When the culture period was extended to 48 h, the effect of cholesterol was still more pronounced, with the percent of cells with fragmented DNA reaching 53.5% for 40 µM and 84.3% for 80 µM. Chromatin condensation of IEC-6 cells was observed after treatment with cholesterol even at 20 µM. Cholesterol did not affect HMG-CoA reductase expression. A dose-dependent increase in PPARγ expression in fetal rat enterocytes was observed. The expression of PPAR-γ was raised by 7-and 40-fold, in the presence and absence of fetal calf serum, respectively, with cholesterol at 80 mM. The apoptotic effect of cholesterol on enterocytes was possibly due to an increase in PPARγ expression.

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Section: Introductionmentioning

confidence: 99%

Cholesterol induces fetal rat enterocyte death in culture

Gazzola

Silva

Kanunfre

et al. 2004

Braz J Med Biol Res

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New Approaches to the Removal of Protein-Bound Toxins from Blood Plasma of Uremic Patients

Sarnatskaya

Yushko

Sakhno

et al. 2007

Artificial Cells, Blood Substitutes, and Biotechnology

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The article is devoted to the theoretical aspects of the development of the effective method for the removal of protein-bound uremic toxins. It is shown that the methods of flow and differential scanning microcalorimetry are sufficient enough for the evaluation of the degree of ligand loading of human serum albumin with protein-bound uremic toxins. The molecules of albumin isolated from blood plasma of the patients being kept on chronic dialysis are demonstrating significant alterations of conformation and complex-forming properties, the correction of which by conventional methods of extracorporeal detoxification (exhaustive dialysis, treatment on synthetic SCN carbons) are practically ineffective. Deliganding of uremic albumin may be successfully performed on conventional carbon haemosorbents upon preliminary separation of blood plasma and its dilution with acetate buffer 1:1 at pH = 5.08. Treatment of the whole blood of patients onto new mass-fractal deliganding carbon, i.e., hemosorbents of HSGD trademark. These HSGD haemosorbents quite effectively could be used for restoration of main parameters of uremic HAS molecules conformation and ligand-binding activity simultaneously with hemodialysis upon the protection by locally performed citrate anticoagulation as an easier and cheaper method for the removal of protein-bound uremic toxins.

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Six years of treatment with the HELP system of a patient with familial hypercholesterolemia

Cited by 2 publications

References 19 publications

Cholesterol induces fetal rat enterocyte death in culture

Cholesterol induces fetal rat enterocyte death in culture

New Approaches to the Removal of Protein-Bound Toxins from Blood Plasma of Uremic Patients

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