Biflavonoids inhibit the production of aflatoxin by Aspergillus flavus

Abstract

The biflavonoids 6,6"-bigenkwanin, amenthoflavone, 7,7"-dimethoxyagastisflavone and tetradimethoxybigenkwanin isolated from Ouratea species were tested for inhibitory activity on Aspergillus flavus cultures. Suspensions of Aspergillus flavus spores were inoculated into 50 ml of YES medium at different biflavonoid concentrations: 5 and 10 µg/ml for 6,6"-bigenkwanin, amenthoflavone and 7,7"-dimethoxyagastisflavone, and 5, 10, 15 and 20 µg/ml for tetradimethoxybigenkwanin. The four biflavonoids showed inhibitory activity on aflatoxin B1 and B2 production (P<0.001), but did not inhibit fungal growth at the concentration tested (P>0.05). These results show that biflavonoids can be used for the development of agents to control aflatoxin production.

Biodiversidade flavonoídica e aspectos farmacológicos em espécies dos gêneros Ouratea e Luxemburgia (Ochnaceae)

“…O óleo extraído do extrato hexânico dos frutos de Ouratea parviflora apresentou atividades antibacteriana e antifúngica 30 . Os biflavonóides isolados de O. spectabilis, O. multiflora e O. parviflora mostraram inibição da produção de aflatoxina por Aspergillus flavus 31 .…”

Inhibitory activity of compounds isolated from Polymnia sonchifolia on aflatoxin production by Aspergillus flavus

“…Some flavonoids and biflavonoids are biologically active against A. flavus and A. parasiticus (7,10,11). In this work, two active flavonoids were isolated from P. sonchifolia leaves.…”

“…2). The chemical analyses of compound 1 showed a higher oxidation pattern than compound 2, and the oxidation pattern of the flavonoids and their position could be responsible by AFB1 production inhibition (7,10). Norton, 1999, reported that highest AFB 1 inhibition was obtained with 3-OH compounds, which were three times more active than the related 3-deoxy compounds.…”

“…Plant extracts, such as those from garlic and onion, effectively retarded growth and aflatoxin production (5). Natural compounds, such as flavonoids, biflavonoids, stilbenes, essential oils and others, were also active in inhibition of aflatoxin production (1,7,10,11,13).…”

Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach

“…The concentration of aflatoxins was determined by photodensitometry (Shimadzu, CS 9000) comparing the area and density of the spot samples with aflatoxins B 1 , B 2 , G 1, and G 2 standards (Sigma Aldrich, USA) (GONÇALEZ et al, 2001). The detection e quantification limits were established how the lowest fluorescence detectable signal and the lowest concentration measured, respectively.…”