Influence of exercise on the activity and the distribution between free and bound forms of glycolytic and associated enzymes in tissues of horse mackerel

Abstract

The effects of short-term burst (5 min at 1.8 m/s) swimming and longterm cruiser (60 min at 1.2 m/s) swimming on maximal enzyme activities and enzyme distribution between free and bound states were assessed for nine glycolytic and associated enzymes in tissues of horse mackerel, Trachurus mediterraneus ponticus. The effects of exercise were greatest in white muscle. The activities of phosphofructokinase (PFK), pyruvate kinase (PK), fructose-1,6-bisphosphatase (FBPase), and phosphoglucomutase (PGM) all decreased to 47, 37, 37 and 67%, respectively, during 60-min exercise and all enzymes except phosphoglucoisomerase (PGI) and PGM showed a change in the extent of binding to subcellular particulate fractions during exercise. In red muscle, exercise affected the activities of PGI, FBPase, PFK, and lactate dehydrogenase (LDH) and altered percent binding of only PK and LDH. In liver, exercise increased the PK activity 2.3-fold and reduced PGI 1.7-fold only after 5 min of exercise but altered the percent binding of seven enzymes. Fewer effects were seen in brain, with changes in the activities of aldolase and PGM and in percent binding of hexokinase, PFK and PK. Changes in enzyme activities and in binding interactions with subcellular particulate matter appear to support the altered demands of tissue energy metabolism during exercise.

Efecto de los nucleótidos CMP y UMP en la fatiga en ratas

“…During exercise one expects an increased production and accumulation of lactate due to the need of degrading rapidly glucose to obtain energy for the muscle (11,12,26,35). In our study this finding was observed in liver tissue of the nontreated group but not in the muscle.…”

“…It has been reported alterations in enzyme activities and binding interactions in fish brain, liver and muscle during exercise. These alterations are dependent on exercise intensity (26).…”

Neurotoxic effect of triazophos on goldfish (Carassius auratus) and tissue specific antioxidant responses

“…LDH activity assay LDH activity was surveyed by following the study of Lushchak et al (2001), assayed by monitoring the change in NADH absorbance at 340 nm. One unit of enzyme activity was defined as the amount of enzyme consuming 1 μmol of substrate per minute.…”

Characterization of non-cytosolic hexokinase activity in white skeletal muscle from goldfish (Carassius auratus L.) and the effect of cold acclimation

“…Its activity has been found and characterized in many fish species and tissues, such as liver, skeletal and cardiac muscles, brain and gills from rainbow trout, horse mackerel, gilthead sea bream and carp [3,6,[29][30][31][32][33][34]. In these studies, HK is generally used as a model for changes induced by a wide range of stresses, such as hypoxia, exercise, carbohydrate tolerance and temperature.…”