The use of filter paper plasticized with polyvinyl alcohol-glutaraldehyde in ELISA

Abstract

F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcoholglutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcoholglutaraldehyde makes this method economically attractive.

Immobilization of Anti-Galectin-3 onto Polysiloxane–Polyvinyl Alcohol Disks for Tumor Prostatic Diseases Diagnosis

“…In our laboratories, several supports have been proposed for biomolecules immobilization: Dacron [42,43]; ferromagnetic Dacron [44,45]; polyvinyl alcohol-glutaraldehyde network [46][47][48]; polyaniline-Dacron composite [49]; plasticized filter paper with polyvinyl alcohol-glutaraldehyde [50].…”

Polysiloxane/PVA-glutaraldehyde hybrid composite as solid phase for immunodetections by ELISA

“…In our laboratories other procedures for antigen covalent immobilization on supports have been proposed such as Dacron (11), ferromagnetic Dacron (12,13), interpenetrated network of PVA-glutaraldehyde (14)(15)(16)(17), filter paper plasticized with PVA-glutaraldehyde (18), and a polyaniline-Dacron composite (19). The optimal values of F1 antigen amount, conjugate dilution and serum titer established for the present method (1.25 µg/disc; 1:4,000 and 3,200, respectively) can be compared to those found for PVA-glutaraldehyde (1.3 µg/disc; 1:6,000 and 1:12,600), plasticized filter paper (1.25 µg/disc; 1:12,000 and 1:6,400), and polyaniline-Dacron (2 µg/disc; 1:4,000 and 1:800).…”

Immobilized Horseradish Peroxidase on Discs of Polyvinyl Alcohol-Glutaraldehyde Coated with Polyaniline

“…Here, the wells of a microplate were used and therefore discs were obtained. However, PVAG was already used for plasticizing filter paper, which was used as a matrix for protein immobilization [18]. The moldability, flexibility, biocompatibility, and adhesive property of PVA make it a versatile material for bioengineering.…”

“…PVAG has been used for protein immobilization because of the plasticity and high surface area of this material [16–18]. The hydrophilic behavior of PVA, on the other hand, interferes in the microenvironment of the immobilized protein when nonpolar substrates/products are involved.…”