Hunting for differentially expressed genes

Vedoy, Cleber Giovane; Bengtson, Mário H.; Sogayar, Mari Cleide

doi:10.1590/s0100-879x1999000700012

Cited by 9 publications

(8 citation statements)

References 28 publications

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“…Several methods have been developed for detection of differentially expressed genes, namely subtractive hybridization, differential display, and microarrays (reviewed in [7,8]). Differential display (DD) reverse-transcription (RT) polymerase chain reaction (PCR) [9] has been used successfully for characterization of host response to bacteria or bacterial components [10][11][12][13][14].…”

mentioning

confidence: 99%

SalmonellaSerotype Typhimurium Infection of Bovine Peyer’s Patches Down‐Regulates Plasma Membrane Calcium‐Transporting ATPase Expression

Santos¹,

Schoffelmeer²,

Tsolis

et al. 2002

J INFECT DIS

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To identify host genes differentially expressed during Salmonella enterica serotype Typhimurium infection, an RNA differential display was made with total RNA extracted from ileal loops that were infected with Salmonella Typhimurium 2.5 h after infection. Down-regulated cDNA was identified in bovine Peyer's patches after infection that was highly homologous to a human plasma membrane calcium-transporting ATPase (PMCA). Differential expression of PMCA, evaluated by Northern analysis, was found to have more than a 4.6-fold decrease in expression of mRNA (size, approximately 5.1 kb). PMCA mRNA was detected by in situ hybridization exclusively within epithelial cells in the Peyer's patches. cDNA (4.4 kb) was amplified by rapid amplification of cDNA ends, cloned, and sequenced and showed a high homology to hPMCA. Bovine PMCA is down-regulated in epithelial cells of Peyer's patches after infection with Salmonella Typhimurium and, subsequently, may influence cellular calcium levels that contribute to the inflammatory processes in the pathogenesis of diarrhea.

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mentioning

confidence: 99%

SalmonellaSerotype Typhimurium Infection of Bovine Peyer’s Patches Down‐Regulates Plasma Membrane Calcium‐Transporting ATPase Expression

Santos¹,

Schoffelmeer²,

Tsolis

et al. 2002

J INFECT DIS

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“…Throughout the years, a number of techniques have been developed to isolate DNA sequences coding for growth-related genes (1). Most methods yield partial cDNA sequences, thus requiring a search for complete coding sequences.…”

Section: Unfolding Of Novel Growth-related Genesmentioning

confidence: 99%

Functional analysis of newly discovered growth control genes: experimental approaches

Flatschart

Sogayar

1999

Braz J Med Biol Res

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A large number of DNA sequences corresponding to human and animal transcripts have been filed in data banks, as cDNAs or ESTs (expression sequence tags). However, the actual function of their corresponding gene products is still largely unknown. Several of these genes may play a role in regulation of important biological processes such as cell division, differentiation, malignant transformation and oncogenesis. Elucidation of gene function is based on 2 main approaches, namely, overexpression and expression interference, which respectively mimick or suppress a given phenotype. The currently available tools and experimental approaches to gene functional analysis and the most recent advances in mass cDNA screening by functional analysis are discussed.

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“…Genes up-or downregulated in response to JH during the Melipona development are still to be identified. The identification of differentially expressed genes has been used to understand the function of genes and the basic molecular mechanisms of distinct biological processes (Vedoy et al 1999). Differential display reverse transcriptase -polymerase chain reaction (DDRT-PCR) has innovated the gene expression field (Liang and Pardee 1992).…”

Section: Introductionmentioning

confidence: 99%

Differentially displayed expressed sequence tags in Melipona scutellaris (Hymenoptera, Apidae, Meliponini) development

Santana

Nunes

Vieira

et al. 2006

An. Acad. Bras. Ciênc.

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We have compared gene expression, using the Differential Display Reverse Transcriptase -Polymerase Chain Reaction (DDRT-PCR) technique, by means of mRNA profile in Melipona scutellaris during ontogenetic postembryonic development, in adult worker, and in both Natural and Juvenile Hormone III-induced adult queen. Six, out of the nine ESTs described here, presented differentially expressed in the phases L1 or L2, or even in both of them, suggesting that key mechanisms to the development of Melipona scutellaris are regulated in these stages. The combination HT11G-AP05 revealed in L1 and L2 a product which matches to thioredoxin reductase protein domain in the Clostridium sporogenes, an important protein during cellular oxidoreduction processes. This study represents the first molecular evidence of differential gene expression profiles toward a description of the genetic developmental traits in the genus Melipona.

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Hunting for differentially expressed genes

Cited by 9 publications

References 28 publications

SalmonellaSerotype Typhimurium Infection of Bovine Peyer’s Patches Down‐Regulates Plasma Membrane Calcium‐Transporting ATPase Expression

SalmonellaSerotype Typhimurium Infection of Bovine Peyer’s Patches Down‐Regulates Plasma Membrane Calcium‐Transporting ATPase Expression

Functional analysis of newly discovered growth control genes: experimental approaches

Differentially displayed expressed sequence tags in Melipona scutellaris (Hymenoptera, Apidae, Meliponini) development

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