Cellular and matrix interactions during the development of T lymphocytes

Owen, J. J. T.; McLoughlin, D.E.; Suniara, Ravinder K.; Jenkinson, Eric J.

doi:10.1590/s0100-879x1999000500008

Cited by 4 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is increasing evidence that ECM molecules including laminin isoforms and their integrin receptors play a crucial role in thymocyte maturation 4,5 , 19,22–24,38–41 . Migration and proliferation are tightly controlled processes in thymocyte differentiation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Developmentally regulated interactions of human thymocytes with different laminin isoforms

Kutlesa¹,

Siler²,

Speiser³

et al. 2002

Immunology

View full text Add to dashboard Cite

SUMMARYThe gene family of heterotrimeric laminin molecules consists of at least 15 naturally occurring isoforms which are formed by five different a, three b and three c subunits. The expression pattern of the individual laminin chains in the human thymus was comprehensively analysed in the present study. Whereas laminin isoforms containing the laminin a1 chain (e.g. LN-1) were not present in the human thymus, laminin isoforms containing the a2 chain (LN-2/4) or the a5 chain (LN-10/11) were expressed in the subcapsular epithelium and in thymic blood vessels. Expression of the laminin a4 chain seemed to be restricted to endothelial cells of the thymus, whereas the LN-5 isoform containing the a3 chain could be detected on medullary thymic epithelial cells and weakly in the subcapsular epithelium. As revealed by cell attachment assays, early CD4x CD8 x thymocytes which are localized in the thymus beneath the subcapsular epithelium adhered strongly to LN-10/11, but not to LN-1, LN-2/4 or LN-5. Adhesion of these thymocytes to LN-10/11 was mediated by the integrin a6b1. During further development, the cortically localized CD4 + CD8 + thymocytes have lost the capacity to adhere to laminin-10/11. Neither do these cells adhere to any other laminin isoform tested. However, the more differentiated single positive CD8 + thymocytes which were mainly found in the medulla were able to bind to LN-5 which is expressed by medullary epithelial cells. Interactions of CD8+ thymocytes with LN-5 were integrin a6b4-dependent. These resultsshow that interactions of developing human thymocytes with different laminin isoforms are spatially and developmentally regulated.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The more differentiated CD4 + or CD8 + single positive (SP) thymocytes are mainly found in the medulla 3 . There is growing evidence that extracellular matrix molecules might play a fundamental role in localizing the different thymocyte stages in the thymus 4,5 …”

Section: Introductionmentioning

confidence: 99%

Developmentally regulated interactions of human thymocytes with different laminin isoforms

Kutlesa¹,

Siler²,

Speiser³

et al. 2002

Immunology

View full text Add to dashboard Cite

show abstract

“…Instead, much of the secreted cytokines is bound by extracellular matrix components. IL‐7 is produced by bone marrow stromal cells and dendritic cells, where it is secreted and can be bound by extracellular matrix components to provide a localized surface for B‐cell interaction (87, 88). Likewise, BLyS can be released as a soluble ligand for BAFF‐R or BCMA, but much remains bound to the surface of the BlyS‐expressing cell (89).…”

Section: Pathways To B‐cell Homeostasis Rely On Extrinsic Signalsmentioning

confidence: 99%

B‐cell homeostasis: digital survival or analog growth?

Rathmell

2004

Immunological Reviews

View full text Add to dashboard Cite

Maintenance of B-lymphocyte homeostasis requires balanced cell production, death, and proliferation. To coordinate these processes, B cells are dependent on cell extrinsic signals. In lymphocyte development, precursor cells are dependent on Fms-like tyrosine kinase ligand 3 (Flt3L), and pre-B cells are dependent on the cytokine interleukin-7. Transitional B cells require B-lymphocyte stimulator (BLyS) for survival. Mature B cells require B-cell receptor (BCR) signals and also remain sensitive to their microenvironment. An emerging model suggests that extrinsic signals do not regulate B-cell survival through a digital mechanism where cells are simply instructed to survive or die. Instead, availability and competition for extrinsic signals regulates cellular physiology and metabolism in an analog fashion that then influences cell commitment to apoptosis or proliferation. Decreases in cellular metabolism may sensitize cells to activation and action of the pro-apoptotic Bcl-2 family members, Bak and Bax, and promote apoptosis. In contrast, increases in metabolism may predispose cells to proliferate. Analog control of cell physiology can, thus, be integrated with other inputs by individual cells to produce a fate decision for survival, proliferation, or apoptosis and prevent diseases of cell death, such as immunodeficiency, and cell activation and proliferation, such as autoimmunity or cancer.

show abstract

Functional Assessment of α_Eβ₇/E‐cadherin Interactions in the Steady State Postnatal Thymus

Prockop

Petrie

2004

Journal of Immunology Research

View full text Add to dashboard Cite

T cell differentiation in the thymus depends on sequential interactions between lymphoid progenitors and stromal cells in discrete regions of the cortex. Here, we show that despite αEβ7 expression by a subset of the earliest intrathymic precursors (and E-cadherin expression by thymic stroma), interaction of these elements is not required for proper localization of early progenitors into the cortex, or for successful steady state differentiation. These findings indicate that despite in vitro data demonstrating αEβ7 mediated adhesion and proliferation of intrathymic T cell precursor populations, T lymphocyte development can proceed independently of αEβ7/E-cadherin interactions.

show abstract

Cellular and matrix interactions during the development of T lymphocytes

Cited by 4 publications

References 16 publications

Developmentally regulated interactions of human thymocytes with different laminin isoforms

Developmentally regulated interactions of human thymocytes with different laminin isoforms

B‐cell homeostasis: digital survival or analog growth?

Functional Assessment of α_Eβ₇/E‐cadherin Interactions in the Steady State Postnatal Thymus

Contact Info

Product

Resources

About

Cellular and matrix interactions during the development of T lymphocytes

Cited by 4 publications

References 16 publications

Developmentally regulated interactions of human thymocytes with different laminin isoforms

Developmentally regulated interactions of human thymocytes with different laminin isoforms

B‐cell homeostasis: digital survival or analog growth?

Functional Assessment of αEβ7/E‐cadherin Interactions in the Steady State Postnatal Thymus

Contact Info

Product

Resources

About

Functional Assessment of α_Eβ₇/E‐cadherin Interactions in the Steady State Postnatal Thymus