Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells

Ribeiro‐Dias, Fátima; Russo, Marco; Nascimento, Flávia R.F.; Barbuto, José Alexandre Marzagão; Timenetsky, Jorge; Jancar, Sônia

doi:10.1590/s0100-879x1998001100010

Cited by 10 publications

(5 citation statements)

References 13 publications

(8 reference statements)

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“…However, Roder et al [1] showed that macrophages can also exhibit cytotoxic activity against YAC-1 cells if they are activated by supernatants from mitogen-stimulated spleen cell cultures. Preliminary results from our laboratory showed that thioglycollate-elicited macrophages are cytotoxic to YAC-1 cells infected with M. arginini [16]. In this study we further investigated the cytotoxic activity of macrophages induced by M. arginini by measuring NO and TNF release and the contribution of these molecules to the macrophage cytotoxic activity.…”

Section: Introductionmentioning

confidence: 95%

Mycoplasma arginini enhances cytotoxicity of thioglycollate-elicited murine macrophages toward YAC-1 tumor cells through production of NO

Ribeiro‐Dias

Russo

Barbuto

et al. 1999

Journal of Leukocyte Biology

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Bacterial products stimulate macrophage tumoricidal activity through release of tumor necrosis factor (TNF) and nitric oxide (NO). We show here that thioglycollate-elicited macrophages acquire cytotoxic activity when cocultured with Mycoplasma arginini-infected YAC-1 tumor cells and release TNF and NO. Fixed mycoplasmainfected cells, supernatants from infected-cell cultures, or purified heat-killed mycoplasma obtained from cell-free cultures were all able to induce TNF and NO production. Thus, the mycoplasma per se and not a product of infected cells induce the release of these molecules. Addition of prostaglandin E 2 (PGE 2 ) to the cocultures, which reduced TNF release, or antibodies to TNF, did not affect macrophage cytotoxicity nor NO release. Inhibition of NO production by L-NAME or aminoguanidine reduced the cytotoxicity, and treatment with a NO donor was toxic to YAC-1 cells. These results indicate that M. arginini activates thioglycollateelicited murine macrophages for NO and TNF release increasing their cytotoxic activity toward YAC-1 cells and that this activity is dependent on NO but not TNF release. J. Leukoc. Biol. 65: 808-814; 1999.

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Section: Introductionmentioning

confidence: 95%

Mycoplasma arginini enhances cytotoxicity of thioglycollate-elicited murine macrophages toward YAC-1 tumor cells through production of NO

Ribeiro‐Dias

Russo

Barbuto

et al. 1999

Journal of Leukocyte Biology

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“…Several studies have documented M-mediated tumor cell cytotoxicity using in vitro studies of mouse peritoneal Ms [85][86][87][88]. In fact, this method of M investigation is often used to determine efficacy of various chemotherapeutic and biologic interventions [89,90].…”

Section: Importance Of Macrophage In Tumor Defensementioning

confidence: 99%

Intraperitoneal macrophages and tumor immunity: A review

Jackson

Evans

2000

J. Surg. Oncol.

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The macrophage (Mphi) is considered the first line of defense in immune response to foreign invaders. Increasing evidence suggests that Mphi(s) also play an important role against neoplastic cells. Mphi(s) exposed to supraphysiologic concentrations of CO(2) are suppressed. As surgeons apply newer minimally invasive techniques to oncologic therapies, it is important to evaluate the impact of these techniques on host-tumor interactions. We review the current understanding of Mphi biology with specific attention on cytotoxicity in addition to tumor immunity. Although systemic immune function is better preserved after laparoscopy than laparotomy, peritoneal Mphi(s) show reduced function after CO(2) pneumoperitoneum than exposure to air. Mphi(s) have shown cytotoxicity to syngeneic cancer cells and may play an important role in tumor surveillance. The impairment in Mphi function after CO(2) exposure may have an effect on outcome after oncologic surgery. In our understanding, Mphi(s) help destroy neoplastic cells. As CO(2) impairs Mphi activity, laparoscopy may significantly alter the host-tumor interaction.

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“…The effects of ZnO NPs on the viability and proliferation of HepG2 cells (purchased from institute Pastor, Iran) was investigated using the MTT assay (18). For this purpose, the HepG2 cells were seeded at a density of 1 × 10 4…”

Section: Cell Cytotoxicity Assaymentioning

confidence: 99%

Novel Formulated Zinc Oxide Nanoparticles Reduce Hwp1 Gene Expression Involved in Biofilm Formation in Candida albicans with Minimum Cytotoxicity Effect on Human Cells

Aslani

Mohammadi

Roudbary

2018

Jundishapur J Microbiol

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Background: Drug resistance in Candida species, has emerged as a major problem in the public health system worldwide. Application of nanoparticles is proposed as a novel and potential agent for reduction of drug resistance burdens. Objectives: The current study was conducted to evaluate the effects of zinc oxide nanoparticles (ZnO NPs containing chitosan and linoleic acid) on hyphae cell wall proteins (Hwp1) gene expression, a crucial gene in pathogenicity of Candida albicans, and cytotoxicity on human hepatocyte carcinoma (HepG2) cells as well as the production of Reactive Oxygen Species (ROS). Methods: The effects of novel ZnO NPs on expression of Hwp1 gene of C. albicans was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR) in comparison to fluconazole as a standard drug. Reactive Oxygen species production was examined in macrophages treated with ZnO NPs relative to non-treated cells. Also, the cytotoxicity effects of ZnO NPs were assessed using the MTT assay against HepG2 cell line. Results: The findings indicated that ZnO NPs significantly decreased the level of Hwp1 gene expression in standard and clinical isolates of C. albicans. Increased level of ROS production in macrophages was found in the presence of ZnO NPs in concentrationdependent manner compared to the control group without exposure of ZnO NPs (P = 0.001). Furthermore, ZnO NPs did not show cytotoxicity activity on HepG2 cells at different concentrations (P > 0.05). Conclusions: Taken together, the newly synthesized ZnO NPs may be a suitable candidate for inhibition of the critical gene responsible for biofilm dispersion and the control of Candida infection with limited cytotoxicity on human cells. However, more studies are required for support of its effect in vitro and in vivo.

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Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells

Cited by 10 publications

References 13 publications

Mycoplasma arginini enhances cytotoxicity of thioglycollate-elicited murine macrophages toward YAC-1 tumor cells through production of NO

Mycoplasma arginini enhances cytotoxicity of thioglycollate-elicited murine macrophages toward YAC-1 tumor cells through production of NO

Intraperitoneal macrophages and tumor immunity: A review

Novel Formulated Zinc Oxide Nanoparticles Reduce Hwp1 Gene Expression Involved in Biofilm Formation in Candida albicans with Minimum Cytotoxicity Effect on Human Cells

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