Distribution of 131I-labeled Bothrops erythromelas venom in mice

Abstract

Bothrops erythromelas is responsible for many snake bites in northeastern Brazil. In the present study we determined the in vivo distribution of the venom following its subcutaneous injection into mice. B. erythromelas venom and albumin were labeled individually with 131 I by the chloramine T method, and separated in a Sephacryl ® S-200 column. The efficiency of labeling was 68%. Male Swiss mice (40-45 g), which had been provided with drinking water containing 0.05% KI over a period of 10 days prior to the experiment, were inoculated dorsally (sc) with 0.3 ml (2.35 x 10 5 cpm/mouse) of 131 I-venom (N = 42), 131 I-albumin or 131 I (controls, N = 28 each). Thirty minutes and 1, 3, 6, 12, 18 and 24 h after inoculation, the animals were perfused with 0.85% NaCl and skin and various organs were collected in order to determine radioactivity content. There was a high rate of venom absorption in the skin (

A biodistribution study of Hemiscorpius lepturus scorpion venom and available polyclonal antivenom in rats

“…In order to make more accurate estimation of our findings, radiolabeled albumin method will need to be carried out in future studies (23). The radioactivity uptake in the small intestine increased until 60 minutes (3.91%ID/g) and then decreased slowly.…”

An overview of Bothrops erythromelas venom

“…Rocha et al (35) continued the studies conducted by Vasconcelos et al (15) by investigating the plasma pharmacokinetics of B. erythromelas venom labeled with 131 I in the presence and absence of an antivenom in mice. In the presence of the antivenom, the percentage radioactivity of B. erythromelas in plasma was higher and its elimination half-life was longer than the respective values were in the absence of the antivenom.…”

“…In 1998, Vasconcelos et al (15) studied the in vivo distribution of B. erythromelas venom. In that study, the venom was labeled with 131 I and administered subcutaneously to mice.…”

Ação cardiovascular da peçonha de Bothrops jararacussu e Bothropstoxina em ratos anestesiados

“…A sensitividade do pulmão esta em concordância com o importante dano pulmonar causado por peçonhas das serpentes do gênero Bothrops e seus componentes em animais de laboratório (71,107) e em observações clinicas de hemorragia pulmonar (89). Porem, apesar da susceptibilidade a danos, não há preferência aparente na acumulação da peçonha ou seus componentes no tecido pulmonar (108,109,110,74). Os rins são um local importante para ação e eliminação da peçonha ( 102), e falha renal aguda pode ser desenvolvida por espécies botrópicas (94).…”