Neutralizing capacity of a new monovalent anti-Bothrops atrox antivenom: comparison with two commercial antivenoms

Otero, Rafael; Núñez, Vitelbina; Gutiérrez, José Marı́a; Robles, Arodys; Estrada, Ricardo; Osorio, R.G.; Del-Valle, G.; Valderrama, Rafael; Giraldo, César Augusto Giraldo

doi:10.1590/s0100-879x1997000300011

Cited by 16 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Colombia, two whole IgG polyvalent antivenoms, manufactured by Instituto Nacional de Salud (INS; neutralizing potency 70 mg B. asper venom per 10 ml vial) and Probiol (neutralizing potency 25 mg B. asper venom/vial), and one F(ab 0 ) 2 antivenom imported from Mexico (Bioclon; neutralizing potency 30 mg B. asper venom/vial) are produced and/or distributed (Table 2). In addition, antivenoms from Brazil, Costa Rica, and Venezuela, with differences in potency, have neutralizing capacity against B. asper venom from Colombia, as demonstrated experimentally (Otero et al , 1997(Otero et al , 2002b) and clinically (Otero-Patiño et al 2012).…”

Section: Specific Treatment Bothropic Envenomationmentioning

confidence: 98%

Snakebites in Colombia

Otero-Patiño

2014

Toxinology

View full text Add to dashboard Cite

Nearly 4,000 snakebites are annually reported in Colombia -90-95 % are inflicted by pit vipers from Bothrops, Porthidium, Bothriechis, Bothriopsis, and Bothrocophias genera, specially by B. asper (50-80 %); 2 % by the bushmaster Lachesis spp.; 1 % by the rattle snake Crotalus durissus cumanensis; 1 % by coral snakes (Micrurus spp.); and the remaining 1-5 % by snakes of aglyphous and opisthoglyphous dentition, mainly from Colubridae family. The Colombian fatality rate of snakebite envenoming is 1-3 %, the highest percentage in the Orinochian and Amazonian regions, whereas 6 % of patients suffer some type of sequelae, mainly as a result of dermonecrosis and myonecrosis. B. asper is responsible for 60-90 % of deaths secondary to snakebites. In this chapter, the clinical and epidemiological aspects of bothropic, lachesic, crotalic, and elapidic envenomations are described, with specific features for the country, as well as the actual knowledge of venom composition and actions.

show abstract

Section: Specific Treatment Bothropic Envenomationmentioning

confidence: 98%

Snakebites in Colombia

Otero-Patiño

2014

Toxinology

View full text Add to dashboard Cite

show abstract

“…Secondary infection, compartmental syndrome, and extensive necrosis can lead to temporary or permanent disability of the bitten limb. Spontaneous systemic bleeding and acute renal failure are common complications from B. atrox envenomings [3,4]. However, the occurrence of each sign/symptom is variable among the patients.…”

Section: Introductionmentioning

confidence: 99%

The relationship between clinics and the venom of the causative Amazon pit viper (Bothrops atrox)

et al. 2020

View full text Add to dashboard Cite

Snake venoms are complex mixtures of proteins with toxic activities, with many distinct isoforms, affecting different physiological targets, comprised in a few protein families. It is currently accepted that this diversity in venom composition is an adaptive advantage for venom efficacy on a wide range of prey. However, on the other side, variability on isoforms expression has implications in the clinics of human victims of snakebites and in the efficacy of antivenoms. B. atrox snakes are responsible for most of the human accidents in Brazilian Amazon and the type and abundance of protein families on their venoms present individual variability. Thus, in this study we attempted to correlate the individual venom proteome of the snake brought to the hospital by the patient seeking for medical assistance with the clinical signs observed in the same patient. Individual variability was confirmed in venoms of the 14 snakes selected for the study. The abundance of each protein family was quite similar among the venom samples, while the isoforms composition was highly variable. Considering the protein families, the SVMP group presented the best correlation with bleeding disorders and edema. Considering individual isoforms, some isoforms of venom metalloproteinase (SVMP), C-type lectin-like toxins (CTL) and snake venom serine proteinases (SVSP) presented expression levels that with statistically significant positive correlation to signs and symptoms presented by the patients as bleeding disorders, edema, ecchymosis and blister formation. However, some unexpected data were also observed as the correlation between a CTL, CRISP or LAAO isoforms with blister formation, still to be confirmed with a larger number of samples. Although this is still a small number of patient samples, we were able to indicate that venom composition modulates clinical manifestations of snakebites, to confirm at the bedside the prominent role of SVMPs and to include new a1111111111 a1111111111 a1111111111 a1111111111 a1111111111

show abstract

“…También es importante tener en cuenta factores epidemiológicos, cantidad de veneno producido y de su consecución, el perfil toxicológico de los venenos, y por supuesto, la eficacia y la seguridad de los antivenenos (9)(10)(11). Estos biológicos neutralizan bien las especies antes descritas tanto en su capacidad letal, hemorrágica, formación de edema, capacidad defibrinante y miotóxica, aunque con diferencias entre las diferentes casas productoras (14,15). También hay que decir que la capacidad de neutralización disminuye en la medida que se aumenta la exposición a diferentes antígenos, es así como entre mayor número de especies se intente abarcar en el antiveneno, menor será su efectividad (5).…”

Section: Introductionunclassified

Sueros antiofídicos en Colombia: análisis de la producción, abastecimiento y recomendaciones para el mejoramiento de la red de producción

Gómez-Cardona

Gómez-Cabal²,

Gómez-Cabal³

2017

biosalud

View full text Add to dashboard Cite

La ofidiotoxicosis o accidente ofídico es un evento frecuente en Colombia. En el país hay dos familias de serpientes de importancia clínica y epidemiológica, las víboras y las corales. Para el tratamiento de la ofidiotoxicosis es obligatoria la utilización de la terapia antivenenosa; los sueros son fabricados a partir de inmunoglobulinas de caballo, estos productos a pesar de que fueron descubiertos hace más de 100 años todavía hoy están en constante cambio. Hoy su industria requiere ser altamente tecnificada, con procesos de eficacia, seguridad y con altos estándares de calidad en Colombia. Los sueros antiofídicos no son productos químico-farmacéuticos, son biológicos, por lo tanto no se caracterizan por su composición química sino por sus propiedades terapéuticas, estos se clasifican en monovalentes y polivalentes, que según su composición física permite neutralizar diferentes tipos de venenos. En el país existen dos productores nacionales (Instituto Nacional de Salud y Laboratorios Probiol) y uno importado de México (Bioclon); la capacidad neutralizante y calidad varía según su composición y proceso de fabricación asociado a la cantidad de proteínas y agregados que influenciarán en el tipo y cantidad de reacciones adversas al medicamento. Se pretende contextualizar al lector en el proceso de fabricación, en la problemática de la escasez y calidad de los sueros antiofídicos en Colombia, dando valoración de conceptos y presentando fundamentos teóricos que aporten a esta temática, además de opinar sobre sobre la actual coyuntura por la escasez de este producto en Colombia.

show abstract

Neutralizing capacity of a new monovalent anti-Bothrops atrox antivenom: comparison with two commercial antivenoms

Cited by 16 publications

References 12 publications

Snakebites in Colombia

Snakebites in Colombia

The relationship between clinics and the venom of the causative Amazon pit viper (Bothrops atrox)

Sueros antiofídicos en Colombia: análisis de la producción, abastecimiento y recomendaciones para el mejoramiento de la red de producción

Contact Info

Product

Resources

About