Transient expression of a reporter gene introduced by bioballistic bombardment into Racosperma mangium (Leguminosae family) tissues

Abstract

We report on an assay of direct transfer of DNA into calli and seeds of Racosperma (ex-Acacia) mangium, using a bioballistic method. We observed transient expression of the GUS gene in the treated tissues<br>Descreve-se um experimento de introdução de DNA em calos e sementes de Racosperma (ex-Acacia) mangium, utilizando um método biobalístico. A expressão do gene marcador GUS foi observada nos dois tipos de tecido

A NewSenegalia(Fabaceae, Mimosoideae) from Southern Peru

“…Indeed, apart from his own publications (Pedley 1987a(Pedley -d, 1988) there appear to be few others that have adopted the name Racosperma. The only papers known to us are Khasa (1993), Khasa & Bousquet (1994) and (1995), Khasa et al (1994aKhasa et al ( ), (1994bKhasa et al ( ), (1995a and (1995b), Quoirin et al (1997), Carvalho et al (1999) and Takemori et al (2000). These are outweighed, many times over (far too many to list here), by papers adopting the name Acacia, including many in which the Racosperma combinations are cited in synonymy.…”

Factors affecting somatic embryogenesis and transformation in modern plant breeding

“…Other biolistic parameters shown to affect transformation efficiency include helium pressure, macrocarrier flight distance to baffle screen, distance from baffle screen to target tissues and vacuum pressure. The factors which affect the delivery and transient expression of the gene have been studied and optimized for different species (Quoirin et al, 1997;Marchant et al, 1998;Deroles et al, 2002;Janna et al, 2006). It is apparent that optimal parameters for transformation efficiency must be arrived at empirically for each species.…”