Chromosome studies in some Stevia. Cav. (Compositae) species from Southern Brazil

Frederico, Adriana P.; Ruas, Paulo Maurício; Marin-Morales, Maria Aparecida; Ruas, Claudete de Fátima; Nakajima, Jimi Naoki

doi:10.1590/s0100-84551996000400013

Cited by 21 publications

(27 citation statements)

References 11 publications

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“…For S. rebaudiana, the chromosome number of 2n = 22 (n = 11) previously reported by Frederico et al (1996) and Monteiro (1980Monteiro ( , 1982 was confirmed here for various strains (Table 1). However, two strains had 2n = 33 and 2n = 44, and represented triploid and tetraploid cytotypes, respectively (Table 1).…”

Section: Discussionsupporting

confidence: 85%

“…Details of the chromosomal morphology of six Brazilian species of Stevia, including S. rebaudiana, were recently published by Frederico et al (1996). The karyotypes were very similar in chromosome number (2n = 22) and size (1.0-2.4 µm).…”

Section: Discussionmentioning

confidence: 96%

“…Although most reports indicate that n = 11 (2n = 22), values of 2n = 24, 33, 34, 44, 48, 66, 70 have also been observed (Darlington and Wylie, 1955;Bolkhoviskikh et al, 1969;Moore, 1973Moore, , 1974Moore, , 1977Golblatt, 1981Golblatt, , 1984Golblatt, , 1985Golblatt, , 1988Goldblatt and Johnson, 1990, 1991, 1996, 1998. Galiano (1987) considered Stevia as a multibasic genus, with x = 11, x = 12 and x = 17, while Frederico et al (1996) considered its basic chromosome number as x = 11. According to This variation reflects numerical (aneuploidy and polyploidy) and structural changes, mainly pericentric inversions (Frederico et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

“…Galiano (1987) considered Stevia as a multibasic genus, with x = 11, x = 12 and x = 17, while Frederico et al (1996) considered its basic chromosome number as x = 11. According to This variation reflects numerical (aneuploidy and polyploidy) and structural changes, mainly pericentric inversions (Frederico et al, 1996). Aneuploidy also occurs in Picris babylonica, a plant that belongs to the same family as Stevia (Malallah et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

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Chromosomal and morphological studies of diploid and polyploid cytotypes of Stevia rebaudiana (Bertoni) Bertoni (Eupatorieae, Asteraceae)

et al. 2004

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In this study, we examined the chromosome number and some morphological features of strains of Stevia rebaudiana. The chromosomes were analyzed during mitosis and diakinesis, and the tetrad normality and pollen viability were also assessed. In addition, stomata and pollen were measured and some plant features were studied morphometrically. All of the strains had 2n = 22, except for two, which had 2n = 33 and 2n = 44. Pairing at diakinesis was n = 11 II for all of the diploid strains, whereas the triploid and tetraploid strains had n = 11 III and n = 11 IV , respectively. Triploid and tetraploid plants had a lower tetrad normality rate than the diploids. All of the strains had inviable pollen. Thus, the higher the ploidy number, the greater the size of the pollen and the stomata, and the lower their number per unit area. The triploid strain produced the shortest plants and the lowest number of inflorescences, whereas the tetraploid strain had the largest leaves. Analysis of variance revealed highly significant differences among the strains, with a positive correlation between the level of ploidy and all of the morphological features examined.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Chromosomal and morphological studies of diploid and polyploid cytotypes of Stevia rebaudiana (Bertoni) Bertoni (Eupatorieae, Asteraceae)

et al. 2004

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“…Variations in the chromosomal number have been reported for many groups of Astereaceae, including diploidy as in Mikania (Maffei et al, 1999) and polyploidy as in Helianthus (Vanzela et al, 2002); other groups, such as Stevia, show numerical stability (Frederico et al, 1996).…”

Section: Resultsmentioning

confidence: 99%

A karyotypic study of three southern Brazilian Asteraceae species using fluorescence in situ hybridization with a 45S rDNA probe and C-CMA3 banding

2004

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The Asteraceae, one of the largest families of flowering plants, contains about 1,100 genera and 20,000 species, and is well known for its extensive karyotypic variation. In this study, conventional Feulgen staining, C-CMA 3 banding, and fluorescence in situ hybridization with a 45S rDNA probe were used to determine the chromosome number and the number and physical position of GC-rich heterochromatin and 45S rDNA sites in three Asteraceae weed species (Crepis japonica, Galinsoga parviflora and Chaptalia nutans). The three species exhibited karyotype differences in the chromosome number and shape, as a commom feature of Asteraceae. However, the 45S rDNA sites always occurred on the short chromosomal arms, associated with GC-heterochromatin. Althought of these differences, it suggests that commom features of plant karyotype are maintained.

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Stevia

Singh

Dhir

2008

Compendium of Transgenic Crop Plants

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Stevia ( Stevia rebaudiana (Bertoni)) is a small bush native to Paraguay. Stevia has been traditionally used as a medicinal herb for hundreds of years in various parts of the world. Stevia leaves are a rich source of a natural sweetener “stevioside” which is up to 300 times sweeter than sucrose. Stevia products are primarily known to be used as artificial low‐calorie sweetener while other important characteristics include antimicrobial, antibacterial, antiviral, and antiyeast activity. However, studies have shown that the stevioside content of leaves can vary substantially (5–20%) between individual plants. Such inherent natural variability could be partially explained by self‐incompatible nature of the flowers. Moreover, it is difficult to increase the commercial scale production by conventional means of sexual seed production due to poor seed germination. In addition, limited vegetative propagation is also a major constraint in Stevia production. The worldwide demand for high potency sweeteners is expected to increase in the near future that implicates developing alternative methods for rapid production of plants with high and uniform yields of sweeteners. Various efforts including in vitro clonal propagation, gene transfer using cutting edge DNA delivery systems, rapid production and high frequency regeneration of transgenic homogenous population plants have been discussed in detail. Maximizing Stevia production through unconventional methods will unleash its potential to become an important nutritional and therapeutic supplement for the growing natural food market in the future.

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Chromosome studies in some Stevia. Cav. (Compositae) species from Southern Brazil

Cited by 21 publications

References 11 publications

Chromosomal and morphological studies of diploid and polyploid cytotypes of Stevia rebaudiana (Bertoni) Bertoni (Eupatorieae, Asteraceae)

Chromosomal and morphological studies of diploid and polyploid cytotypes of Stevia rebaudiana (Bertoni) Bertoni (Eupatorieae, Asteraceae)

A karyotypic study of three southern Brazilian Asteraceae species using fluorescence in situ hybridization with a 45S rDNA probe and C-CMA3 banding

Stevia

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