Conservação e germinação in vitro de pólen de milho (Zea mays subsp. mays)

Amaral, António; Neto, José Fernandes Barbosa; Sereno, Maria Jane Cruz de Melo

doi:10.1590/s0100-84042011000400003

Cited by 12 publications

(11 citation statements)

References 10 publications

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“…This value was independent of the genetic material used, the in vitro germination rate, and the production of seeds per spike. In other studies of maize pollen viability, the authors discuss the loss of pollen viability as the pollen grains are dried (Almeida et al, 2011;Alvim, 2012). Geiger and Schönleben (2011) found that haploid plants may produce a fertile tassel, but in an excessively small percentage of plants.…”

Section: Discussionmentioning

confidence: 99%

Verification and characterization of chromosome duplication in haploid maize

Couto¹,

Pinho²,

Pinho³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Doubled haploid technology has been used by various private companies. However, information regarding chromosome duplication methodologies, particularly those concerning techniques used to identify duplication in cells, is limited. Thus, we analyzed and characterized artificially doubled haploids using microsatellites molecular markers, pollen viability, and flow cytometry techniques. Evaluated material was obtained using two different chromosome duplication protocols in maize seeds considered haploids, resulting from the cross between the haploid inducer line KEMS and 4 hybrids (GNS 3225, GNS 3032, GNS 3264, and DKB 393). Fourteen days after duplication, plant samples were collected and assessed by flow cytometry. Further, the plants were transplanted to a field, and samples were collected for DNA analyses using microsatellite markers. The tassels were collected during anthesis for pollen viability analyses. Haploid, diploid, and mixoploid individuals were detected using 7000 E.G. de Oliveira Couto et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 6999-7007 (2015) flow cytometry, demonstrating that this technique was efficient for identifying doubled haploids. The microsatellites markers were also efficient for confirming the ploidies preselected by flow cytometry and for identifying homozygous individuals. Pollen viability showed a significant difference between the evaluated ploidies when the Alexander and propionic-carmin stains were used. The viability rates between the plodies analyzed show potential for fertilization.

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Section: Discussionmentioning

confidence: 99%

Verification and characterization of chromosome duplication in haploid maize

Couto¹,

Pinho²,

Pinho³

et al. 2015

Genet. Mol. Res.

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“…Sementara itu, perkecambahan serbuk sari jagung varietas Anjou yang dihidrasi pada suhu 22 ºC, RH 30-50% selama 24 jam turun dari 85% menjadi 5%. Menurut Almeida et al (2011) serbuk sari jagung yang dikeringkan menggunakan silika gel selama 48 jam kemudian disimpan pada suhu -20 ºC menghasilkan perkecambahan yang lebih tinggi (72%) jika dibandingkan dengan serbuk sari yang dikeringkan menggunakan CaC 12 H 2 O (57%) setelah 30 hari simpan. Barnabas dan Rajki (1976) melaporkan bahwa serbuk sari jagung genotip Mv 530 yang disimpan dalam nitrogen cair menghasilkan perkecambahan 5% setelah 4 bulan simpan, dan setelah digunakan untuk produksi benih menghasilkan 5 butir per tongkol.…”

Section: Pendahuluanunclassified

Penyimpanan Serbuk Sari Jagung dan Potensinya untuk Produksi Benih Hibrida

2017

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Production of maize hybrid seed is hindered by relatively poor production by the male line pollen; short live of the pollen which was ascribed to desiccation and dehydration sensitive and flowering asynchrony between male and female lines. The aims of this study were to enhance production and viability of male line pollen and also to determine the suitable storage condition for maintaining pollen viability in order to be used for production of hybrid seed. The research consisted of two experiments. The first experiment was arranged in split plot randomized block design with NPK dosages as main plot (0, 300, 600, 900 kg ha -1 ) and application of boron as sub-plot (0, 1.5, 3, 4.5, 6 kg ha -1 ), replicated three times. The second experiment used randomized block design with two factors, i.e. storage temperature (-196 o C and -20 o C) and storage period (0, 1, 2, 3, 4 weeks). The result showed that NPK 900 kg ha -1 increased the number of spike per tassel from 9.3 to 10.7. Application of NPK 600 kg ha -1 or boron 1.5 kg ha -1 improved pollen viability. Combination of NPK 900 kg ha -1 and boron 6 kg ha -1 resulted in the highest pollen viability of 95.3%. Pollen after being stored for four weeks either in -196 ºC or -20 ºC was possible to be used for seed production with 20% seed set. ABSTRAKKendala utama dalam produksi benih jagung hibrida adalah produksi serbuk sari tetua jantan yang rendah, periode viabel serbuk sari yang singkat karena serbuk sari jagung sensitif terhadap desikasi dan dehidrasi, serta asinkroni antara waktu mekar bunga jantan dan betina. Penelitian ini bertujuan meningkatkan produksi dan viabilitas serbuk sari galur tetua jantan, serta mendapatkan kondisi penyimpanan yang dapat memperpanjang viabilitas serbuk sari untuk digunakan dalam produksi benih hibrida. Penelitian ini terdiri atas 2 percobaan. Percobaan pertama menggunakan rancangan petak terbagi dengan rancangan lingkungan acak kelompok. Petak utama adalah dosis NPK (0, 300, 600, 900 kg ha -1 ) dan dosis boron sebagai anak petak (0, 1.5, 3, 4.5, 6 kg ha -1 ), dengan 3 ulangan. Percobaan kedua menggunakan RAK 2 faktor, yaitu suhu penyimpanan (-196 o C dan -20 o C) dan periode simpan (0, 1, 2, 3, 4 minggu). Hasil penelitian menunjukkan bahwa pemupukan NPK 900 kg ha -1 meningkatkan jumlah spika per tasel dari 9.3 buah menjadi 10.7 buah. Aplikasi NPK 600 kg ha -1 atau dosis boron 1.5 kg ha -1 dapat meningkatkan viabilitas serbuk sari jagung. Kombinasi perlakuan dosis NPK 900 kg ha -1 dan boron 6 kg ha -1 menghasilkan viabilitas serbuk sari paling tinggi sebesar 95.3%. Serbuk sari yang disimpan pada suhu -196 °C dan -20 °C selama 4 minggu dapat digunakan untuk produksi benih dengan potensi jumlah benih per tongkol sebesar 20% dari penggunaan serbuk sari segar. Kata kunci: boron, dosis NPK, deep freezer, nitrogen cair, viabilitas serbuk sari

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“…Knowledge about pollen viability is important for taxonomy, ecology and palynology, providing information on the reproductive biology and conservation of the species (Alexander, 1980;Souza et al, 2002;Usman et al, 2013). It is also critical in genetic breeding, in the routine use of artificial pollination (Nascimento et al, 2003;Munhoz et al, 2008); in pollen monitoring during storage; in evaluation of pollen germination after exposure to stresses; in analyses of dispersion and gene flow; and in studies of genetics and pollen-stigma interactions, incompatibility and fertility (Santos et al, 2007;Bauermann et al, 2009;Almeida et al, 2011;Soares et al, 2011;Cuchiara et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

“…Viability of the pollen grain can be determined by methods of cytochemical staining, in vitro germination, in vivo germination, and percentage of effective fructification (Einhardt et al, 2006;Almeida et al, 2011). The staining methods provide rapid, low-cost results, with different dyes being commonly used (Techio et al, 2006), depending on the constitution and morphology of the pollen grain and properties of the dyes.…”

Section: Introductionmentioning

confidence: 99%

Genetic parameters of pollen viability in guava (Psidium guajava L.)

Silva¹,

Silva²,

Marçal³

et al. 2017

Aust J Crop Sci

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Pollen viability is essential for the sexual reproduction in plants. Genetic and environmental factors as well as plant age can influence this characteristic. In this work, pollen viability was studied in guava (Psidium guajava L.), a cross-pollination species. The genetic parameters for this characteristic were estimated considering 22 genotypes, two environments and different plant ages. For that, the pollen viability of the genotypes was evaluated for three years (2013, 2014 and 2015) in two experimental orchards (installed in randomized block design, with three blocks and two plants per plot) and at two different regions of the state of Espírito Santo, Brazil. The plants were analyzed at 19, 24 and 38 months of age in the years 2013 and 2014 in Mimoso do Sul (ES) and 2015 in Linhares (ES), respectively. The flower buds, at pre-anthesis stage, were collected, fixed in ethanol:acetic acid (3:1) and stored at -20°C. Pollen viability was obtained by colorimetric methods (Alexander's, Acetic Orcein and Lugol dyes) and the genetic parameters estimated by means of mixed models. The pollen viability of the genotypes was high, with an overall mean of 93.46% in the thr ee harvests. Mean heritability was lower in the harvests of 2013 (0.479) and 2014 (0.126) in relation to 2015 (0.583), indicating a slighter possibility of predicting genetic gains based on this characteristic. Altogether, these results provide information about pollen viability in commercial and superior genotypes of the guava crop used in this study, given that pollen-donor genotypes are reported to influence characteristics related to weight and fruit quality in this species. In addition, these genotypes showed good potential for cross-pollination, and can therefore be used as pollinators in orchards and crosses within breeding programs.

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Conservação e germinação in vitro de pólen de milho (Zea mays subsp. mays)

Cited by 12 publications

References 10 publications

Verification and characterization of chromosome duplication in haploid maize

Verification and characterization of chromosome duplication in haploid maize

Penyimpanan Serbuk Sari Jagung dan Potensinya untuk Produksi Benih Hibrida

Genetic parameters of pollen viability in guava (Psidium guajava L.)

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