Microsatellite-dense genetic map: towards genome coverage in a tropical maize (Zea mays L.) population

Abstract

-(Microsatellite-dense genetic map: towards genome coverage in a tropical maize (Zea mays L.) population). Dense molecular genetic maps are used for an effi cient quantitative trait loci (QTL) mapping and in the marker-assisted selection programs. A dense genetic map was generated with 139 microsatellite markers using 256 F 2 plants generated by the crossing of two tropical maize inbred lines (L-02-03D and L-20-01F). This map presented 1,858.61 cM in length, where 10 linkage groups were found spanned, with an average interval of 13.47 cM between adjacent markers. Seventy seven percent of the maize genetic mapping bins were covered, which means an increase of 14% coverage in relation to the previous tropical maize maps. The results provide a more detailed and informative genetic map in a tropical maize population representing the fi rst step to make possible the studies of genetic architecture to identify and map QTL and estimate their effects on the variation of quantitative traits, thus allowing the manipulation and use in tropical maize breeding programs.

Genetic analysis of microsatellite markers for salt stress in two contrasting maize parental lines and their RIL population

“…The development and mapping of DNA markers are essential for using QTL mapping of salt tolerance and marker-assisted selection of this trait in maize genotypes. Maize is one of the fi rst major crop species to have had maps of different molecular markers (Davis et al 1999, Sharopova et al 2002, Sibov et al 2003, Lima et al 2009, Xu et al 2013, Zhou et al 2011. Recently, research has focused on genetic dissection in maize using QTL mapping of recombinant inbred lines (RILs) derived from a cross between two maize inbred lines with contrasting salinity tolerance.…”