Antifungal Activity of Aerial Parts of Cenchrus pennisetiformis Against Fusarium oxysporum f. sp. lycopersici

Abstract

Antifungal potential of aerial parts of an allelopathic grass Cenchrus pennisetiformis (Hochst. & Steud.) Wipff. was evaluated against Fusarium oxysporum f. sp. lycopersici Snyder & Hansen, a fungal pathogen causing wilt disease in tomato (Solanum lycopersicum L.). Different concentrations (1% to 6%) of methanolic leaf, stem and inflorescence extract of the grass significantly reduced fungal biomass by 40-88%, 13-89%, and 26-76%, respectively. Methanolic shoot (leaf + stem) extract was fractionated using four organic solvents viz. n-hexane, chloroform, ethyl acetate and n-butanol. All the sub-fractions of methanolic shoot extract showed remarkable antifungal potential to variable extents. Different concentrations (1.56-200 mg mL-1) of ethyl acetate sub-fraction exhibited the best antifungal activity resulting in 49-100% suppression in the fungal biomass. GC-MS analysis of ethyl acetate sub-fraction showed the presence of 10 compounds. Phenol, 2,4-bis{1,1-dimethlethyl}- was the major compound (30.99%) followed by hexadecanoic acid, ethyl-ester (21.72%), benzofuran 2,3-dihydro (10.65%), 1-propanol-2-2-hydroxypropxy (10.60%) and 1-eicosene (8.32%).

Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici

“…Afterwards, the residual extract was sequentially partitioned with chloroform) (400 mL) and ethyl acetate) (400 mL). All the fractions in organic solvents were evaporated on a rotary evaporator to gain 30 g of n-hexane) fraction, 10 g of (chloroform) fraction and 2 g of (ethyl acetate fraction (Khurshid et al, 2018).…”

Management of Charcoal Rot of Mungbean by Two Trichoderma Species and Dry Biomass of Coronopus didymus

“…However, numerous fungicides available in the market are not only toxic but also pollute the environment (Anggriani et al, 2015) and create resistance in fungal pathogens (Kim et al, 2007). A likely alternative to resolve such problems is the use of natural resources, including microorganisms and products from higher plants (De Corato et al, 2014;Ali et al, 2017;Khurshid et al, 2018). Protecting plants against fungal pathogens using such alternatives may also reduce development of resistance in the pathogens (Trigui et al, 2013).…”

Identification of antifungal compounds from slender amaranth

“…1-eicosene reported in the present study was also reported in another study describing antifungal activity of Cenchrus pennisetiformis against Fusarium oxysporum f. sp. lycopersici (Khurshid et al, 2018).…”