Teste rápido de imersão foliar de Euphorbia heterophylla para confirmação de resistência a herbicidas inibidores da Protox e da ALS

Trezzi, Michelangelo Müzell; Xavier, Elouize; Vidal, Ribas Antônio; Portes, Emerson da Silva; Vottri, Marciano; Lamego, Fabiane Pinto

doi:10.1590/s0100-83582011000400021

Cited by 6 publications

(6 citation statements)

References 14 publications

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“…The first known case of resistance to ALS-inhibiting herbicides in this species was reported in Brazil (1993) and some years later in Paraguay (1995) 6 . Since then, other E. heterophylla cases with ALS-inhibiting herbicide resistance (including imazamox) have been found in large areas of Brazil (2004), also selecting for resistance to herbicides with other modes of action (MOA) 11–14 .…”

Section: Introductionmentioning

confidence: 99%

Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype

Rojano-Delgado

Portugal

Palma-Bautista

et al. 2019

Sci Rep

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Euphorbia heterophylla is a weed species that invades extensive crop areas in subtropical regions of Brazil. This species was previously controlled by imazamox, but the continuous use of this herbicide has selected for resistant biotypes. Two biotypes of E. heterophylla from southern Brazil, one resistant (R) and one susceptible (S) to imazamox, were compared. The resistance of the R biotype was confirmed by dose-response assays since it required 1250.2 g ai ha−1 to reduce the fresh weight by 50% versus 7.4 g ai ha−1 for the S biotype. The acetolactate synthase (ALS) enzyme activity was studied using ALS-inhibiting herbicides from five different chemical families. The R biotype required the highest concentrations to reduce this enzyme activity by 50%. A Ser653Asn mutation was found in the ALS gene of the R biotype. The experiments carried out showed that imazamox absorption and metabolism were not involved in resistance. However, greater 14C-imazamox root exudation was found in the R biotype (~70% of the total absorbed imazamox). Target site mutation in the ALS gene is the principal mechanism that explains the imazamox resistance of the R biotype, but root exudation seems to also contribute to the resistance of this biotype.

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Section: Introductionmentioning

confidence: 99%

Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype

Rojano-Delgado

Portugal

Palma-Bautista

et al. 2019

Sci Rep

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“…In recent years, with the detection of new E. heterophylla biotypes suspected of multiple resistance to ALS and PPO inhibitors (Trezzi et al, 2011;Xavier et al, 2013), there is a need to prove this resistance and to investigate its responsible mechanism, in order better manage these biotypes.…”

Section: Introductionmentioning

confidence: 99%

Activity of Antioxidant Enzymes in Euphorbia heterophylla Biotypes and their Relation to Cross Resistance to ALS and Protox Inhibitors

et al. 2018

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The characteristics of multiple resistance in Euphorbia heterophylla biotypes to herbicides that are inhibitors of ALS (Acetolactate synthase) and PPO (Protoporphyrinogen oxidase) and their responsible mechanisms are still not completely elucidated. The objectives of this study were to identify cross-resistance to herbicides from different chemical groups of ALS inhibitors (imidazolinones, sulfonylureas, pyrimidyl benzoates and sulfonanilides) and also PPO inhibitors (diphenylethers, phthalamides, oxadiazoles, triazolinones and pyrimidinediones) in E. heterophylla biotypes with multiple resistance to these herbicides; to analyze whether the antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD) constitute mechanisms that are responsible for the resistance to PPO inhibitors. Initially, the response to doses of herbicides from these different chemical groups was determined, using doses below and above the one recommended for the species. The control of E. heterophylla was determined, estimating the required doses for a 50 and 80% control reduction and calculating the resistance factors. The constitutive and induced activities of the SOD and POD enzymes were also determined. The results confirmed cross-resistance for all chemical groups of ALS and PPO inhibitors in the Bom Sucesso do Sul and Vitorino biotypes. The constitutive and induced activities of the SOD and POD enzymes were superior in plants from the E. heterophylla biotypes Vitorino and Bom Sucesso do Sul, contributing to their resistance to PPO inhibiting herbicides.

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“…No Brasil, a competição da cultura da soja com o leiteiro (Euphorbia heterophylla L.) tem preocupado os agricultores, devido à dificuldade de manejo (Vargas et al, 2013) e os casos de resistência já registrados envolvendo esta espécie (Trezzi et al, 2005;Gelmini et al, 2001) de norte a sul do pais.…”

Section: Introductionunclassified

“…O aumento nos casos de resistência em plantas daninhas decorre, especialmente, da pressão de seleção devido à aplicação constante e abusiva de herbicidas com o mesmo mecanismo de ação (Trezzi et al, 2011;Costa e Rizzardi, 2014). Neste sentido, cabe destacar que a ampla utilização do método químico é consequência da estabilização do sistema de plantio direto, agregação de novas áreas produtivas, a crescente insuficiência de mão-deobra no campo, a elevada eficiência no controle e o alto rendimento das culturas (Christoffoleti et al, 2008;Oliveira Jr. et al, 2011).…”

Section: Introductionunclassified

Avaliação de biótipos de leiteiro com suspeita de resistência a herbicidas inibidores da ALS e Protox

et al. 2014

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Resumo -Objetivou-se com o presente trabalho avaliar a confirmação da ocorrência de resistência de biótipos de leiteiro, oriundos de Rondônia e do Rio Grandes do Sul a herbicidas com diferentes mecanismos de ação, bem como determinar a dose dos herbicidas necessária para redução de 50% na massa seca das plantas (GR50) e para o controle de 50% das plantas de cada unidade experimental (C50). Para tanto foi instalado um experimento em casa de vegetação, em blocos casualizados, com quatro repetições. Os herbicidas utilizados foram: imazethapyr, chlorimuron-ethyl, cloransulam-methyl, saflufenacil, fomesafen e carfentrazone-ethyl. As doses utilizadas foram: 0; 0,5; 2; 8; 16; 32 e 64 vezes a dose comercial. As variáveis avaliadas foram controle do leiteiro aos 07, 14, 21 e 28 dias após a aplicação dos tratamentos (DAT) e também, aos 28 DAT determinou-se a massa seca das plantas. O biótipo do Rio Grande do Sul mostrou sensibilidade aos herbicidas testados, já o de Rondônia apresentou resistência cruzada aos inibidores de ALS (imazethapyr, chlorimuron e cloransulam) e suscetível aos inibidores de PROTOX (carfentrazone, fomesafen e saflufenacil). Palavras-chaves: Euphorbia heterophylla L., Glycine max, plantas daninhasAbstract -The objective of the present study was to evaluate the confirmation of occurrence of wild poinsettia resistance, coming from Rondônia and Rio Grande do Sul, to herbicides with different mechanisms of action, and to determine the dose of herbicides required for 50% reduction in the dry mass of plants (GR50) and for the control of 50% of these plants in each experimental unit (C50). Therefore, an experiment was conducted over greenhouse conditions, in randomized block design with four replications. The herbicides used were: imazethapyr, chlorimuron-ethyl, cloransulam-methyl, saflufenacil, fomesafen, and carfentrazone-ethyl. The doses used were: 0; 0.5; 2; 8; 16; 32 and 64 times the commercial dose. The variables evaluated were control of wild poinsettia at 7, 14, 21, and 28 days after treatments (DAT), and also at 28 DAT was determined the dry mass of plants. The Rio Grande do Sul biotype showed sensitivity to the herbicides tested, 1 Recebido para publicação em 28/07/2014 e aceito em 28/06/2015.

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Teste rápido de imersão foliar de Euphorbia heterophylla para confirmação de resistência a herbicidas inibidores da Protox e da ALS

Cited by 6 publications

References 14 publications

Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype

Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype

Activity of Antioxidant Enzymes in Euphorbia heterophylla Biotypes and their Relation to Cross Resistance to ALS and Protox Inhibitors

Avaliação de biótipos de leiteiro com suspeita de resistência a herbicidas inibidores da ALS e Protox

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