Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype

Rojano-Delgado, Antonia M.; Portugal, J.; Palma-Bautista, Candelario; Cruz, Ricardo Alcántara‐de la; Torra, Joel; Alcántara, Estéban

doi:10.1038/s41598-019-51682-z

Cited by 22 publications

(33 citation statements)

References 62 publications

(70 reference statements)

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“…These results demonstrate the presence of a TSR mechanism for flazasulfuron. Similar studies were conducted in Rapistrum rugosum for different ALS herbicides, in Euphorbia heterophylla and soft wheat for imazamox, and in Conyza canadensis and Epilobium ciliatum for flazasulfuron [38,39,[61][62][63].…”

Section: Discussionsupporting

confidence: 63%

Evolving Multiple Resistance to EPSPS, GS, ALS, PSI, PPO, and Synthetic Auxin Herbicides in Dominican Republic Parthenium hysterophorus Populations. A Physiological and Biochemical Study

et al. 2020

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Two Parthenium hysterophorus populations resistant (R) and susceptible (S) harvested in banana crop from the Dominican Republic were studied. All S plants died when the herbicides were applied at field dose, except with paraquat. For the R population, the order of plant survival was as follows: glyphosate and paraquat > flazasulfuron > glufosinate > fomesafen > 2,4-D. The resistance factors obtained in the dose–response assays showed a high resistance to glyphosate, flazasulfuron, and fomesafen, medium resistance to glufosinate and 2,4-D, and a natural tolerance to paraquat (resistance factor (RF) = 1.0). The I50 values obtained in the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), acetolactate synthase (ALS), and glutamine synthetase (GS) activity studies with glyphosate, flazasulfuron, and glufosinate, respectively, were greater in R than in S. The effect of fomesafen was measured by the Proto IX levels, obtaining five times more Proto IX in the S than in the R population. The resistance to 2,4-D in the R was determined by the lower accumulation of ethylene compared to the S population. The studies with 14C-paraquat conclude that the lower absorption and translocation in both the R and S populations would explain the natural tolerance of P. hysterophorus. This is the first case of multiple resistance to herbicides with different mechanisms of action confirmed in P. hysterophorus.

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Section: Discussionsupporting

confidence: 63%

Evolving Multiple Resistance to EPSPS, GS, ALS, PSI, PPO, and Synthetic Auxin Herbicides in Dominican Republic Parthenium hysterophorus Populations. A Physiological and Biochemical Study

et al. 2020

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“…This high selection pressure led to the emergence of ALS-resistant populations in the late 1990s. Between 1993 and 2004, resistance to ALS was reported for Bidens pilosa, Bidens subalternans , Euphorbia heterophylla and Amaranthus hybridus in countries such as Brazil, Paraguay and Argentina 1 , 2 , and these biotypes are now widespread. ALS- and ACCase inhibitor-resistant weeds were controlled by the addition of protoporphyrinogen oxidase (PPO) inhibitors or glyphosate in glyphosate-resistant (GR) crops 3 .…”

Section: Introductionmentioning

confidence: 99%

Multiple mutations in the EPSPS and ALS genes of Amaranthus hybridus underlie resistance to glyphosate and ALS inhibitors

García

Palma-Bautista

Vázquez-García

et al. 2020

Sci Rep

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Amaranthus hybridus is one of the main weed species in Córdoba, Argentina. Until recently, this weed was effectively controlled with recurrent use of glyphosate. However, a population exhibiting multiple resistance (MR2) to glyphosate and imazamox appeared in a glyphosate resistant (GR) soybean field, with levels of resistance up to 93 and 38-fold higher to glyphosate and imazamox, respectively compared to the susceptible (S) population. In addition to imidazolinones, MR2 plants showed high resistance levels to sulfonylamino-carbonyl (thio) benzoates and moderate resistance to sulfonylureas and triazolopyrimidines. Multiple amino acid substitutions were found in both target genes, acetolactate synthase (ALS) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), responsible for conferring high herbicides resistance levels in this A. hybridus population. In the case of EPSPS, the triple amino acid substitution TAP-IVS was found. In addition, MR2 plants also showed increased EPSPS gene expression compared to susceptible plants. A Ser653Asn substitution was found in the ALS sequence of MR2, explaining the pattern of cross-resistance to the ALS-inhibitor herbicide families found at the ALS enzyme activity level. No other mutations were found in other conserved domains of the ALS gene. This is the first report worldwide of the target site resistance mechanisms to glyphosate and ALS inhibitors in multiple herbicide resistance Amaranthus hybridus.

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“…Some Brazilian weed research groups have partnerships with weed scientists from the United States and Spain and other countries, who collaborate actively in studies that characterize the mechanisms of the most important cases of herbicide resistance of the country [12][13][14][15][16]93]. However, these efforts are still insufficient and the previous information allows us to infer that in most cases, the management of herbicide resistance in Brazil has been faced in an inverse way, i.e., first, efforts are made to test and propose different alternatives to solve the "problem" and, in some cases, then try to characterize the "cause."…”

Section: Scientific and Technical Aspectsmentioning

confidence: 99%

Herbicide Resistance in Brazil: Status, Impacts, and Future Challenges

Cruz¹,

Oliveira²,

Carvalho³

et al. 2020

Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production

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Brazil is a large producer and exporter of crops in global terms. Weeds may be responsible for ~14% of crop losses, depending on the crop system. Herbicides occupy 58% of the Brazilian pesticide market; however, the continuous use of these products and the high selection pressure have led to the emergence of weeds resistant to herbicides. Today, there are 51 weed species reported as being resistant to herbicides in Brazil, of which 17 involves cross and multiple-resistance. Acetolactate synthase (ALS), acetyl coenzyme A carboxylase (ACCase) and 5-enolpiruvylshikimate-3-phosphate synthase (EPSPs) inhibitors are the herbicidal groups with the most resistance cases. Soybean, corn, rice, wheat and cotton present 30, 12, 10, 9 and 8 cases, respectively, occurring mainly in herbicide-resistant crop fields from the Southern and Central West regions of the country. To better understand the dimensions of herbicide resistance, in this chapter, we will explore the size of agricultural activity in Brazil, the pesticide market and the use of herbicides in the main crops. In addition, the agronomic, scientific-technical and economic aspects that have contributed, directly or indirectly, to the selection of resistant weeds will be discussed in order to have an overview of the economic impact of herbicide resistance management.

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Target site as the main mechanism of resistance to imazamox in a Euphorbia heterophylla biotype

Cited by 22 publications

References 62 publications

Evolving Multiple Resistance to EPSPS, GS, ALS, PSI, PPO, and Synthetic Auxin Herbicides in Dominican Republic Parthenium hysterophorus Populations. A Physiological and Biochemical Study

Evolving Multiple Resistance to EPSPS, GS, ALS, PSI, PPO, and Synthetic Auxin Herbicides in Dominican Republic Parthenium hysterophorus Populations. A Physiological and Biochemical Study

Multiple mutations in the EPSPS and ALS genes of Amaranthus hybridus underlie resistance to glyphosate and ALS inhibitors

Herbicide Resistance in Brazil: Status, Impacts, and Future Challenges

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