Atividade de glutationa S-transferase na degradação do herbicida glyphosate em plantas de milho (Zea mays)

Catâneo, A.C.; Déstro, Guilherme Fernando Gomes; Ferreira, L.C.; Chamma, Karina Luiz; Sousa, Daniel Freire de

doi:10.1590/s0100-83582003000200017

Cited by 20 publications

(6 citation statements)

References 22 publications

(16 reference statements)

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“…Seeds subjected to the different concentrations of NaCl, despite having obtained greater activity of GST when compared to seeds subjected to the different osmotic potentials, showed the same tendency ( Figure 2F). The GSTs are considered important enzymes which have the capacity of metabolizing various xenobiotics, thus providing for detoxification of plants, as seen in various studies in wheat, pea, corn, and soybean crops (Uotila et al, 1995, Cataneo et al, 2003, Moldes et al, 2008. The GSTs promote the conjugation of GSH (reduced glutathione) with endogenous cytotoxic products and agents of oxidative damages, like hydroxyl radicals, membrane lipid peroxides, and products of oxidative degradation of DNA, aiming at their detoxification.…”

Section: Resultsmentioning

confidence: 99%

Activity of antioxidant enzymes and proline accumulation in Erythrina velutina Willd. seeds subjected to abiotic stresses during germination

Ribeiro

Matias

Pelacani³

et al. 2014

J. Seed Sci.

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-The aim of this study was to evaluate the effect of different abiotic stresses on the activity of antioxidant enzymes and on accumulation of proline in Erythrina velutina Willd. seeds during germination. Mulungu seeds were scarified and placed to germinate at constant temperatures of 15, 25, and 35 °C, moistened with distilled water, and exposed to 12 h of light. Other seeds were exposed to solutions of NaCl (EC of 0, 4, and 8 dS.m -1 ) and polyethylene glycol (osmotic potentials of 0.0, -0.2, and -0.6 MPa) and maintained in a germination chamber set at 25 °C and 12 h photoperiod for seven days. At the end of each period of imbibition, the embryonic axis and cotyledons of the seedlings were collected separately and used to quantify proline content and the activity of antioxidant enzymes. These were detected in both the cotyledons and embryonic axis of the mulungu seeds. Antioxidant activity varied depending upon the type and degree of stress applied. It was concluded that under the aspect of the detoxification process, the mechanism found in mulungu seeds is more efficient when subjected to different temperatures followed by salt stress and water stress.Index terms: antioxidative metabolism, water stress, salt stress, heat stress, mulungu.Atividade de enzimas antioxidantes e acúmulo de prolina em sementes de Erythrina velutina Willd. submetidas a estresses abióticos durante a germinação RESUMO -Objetivou-se com este trabalho avaliar o efeito de diferentes estresses abióticos na atividade das enzimas antioxidantes e no acúmulo da prolina em sementes de Erythrina velutina Willd. durante a germinação. Sementes de mulungu foram escarificadas e colocadas para germinarem nas temperaturas constantes de 15, 25 e 35 °C, umedecidas com água destilada e com 12 h de luz. Outras sementes foram expostas a soluções teste de NaCl (condutividade elétrica 0, 4 e 8 dS.m -1 ) e soluções de polietilenoglicol (potencias osmóticos 0,0; -0,2 e -0,6 MPa) e mantidas em câmaras de germinação ajustadas a 25 °C, fotoperíodo de 12 h durante sete dias. Os cotilédones e eixo embrionário de plântulas foram coletados e utilizados para quantificar o teor de prolina e a atividade de enzimas antioxidantes. Estas foram detectadas nos cotilédones e eixo embrionário de sementes de mulungu. A atividade antioxidante foi variável dependendo do tipo e nível de estresse aplicado. Os resultados encontrados permitem concluir que sob o aspecto do processo de desintoxicação, o mecanismo encontrado nas sementes de mulungu é mais eficiente quando submetidas a diferentes temperaturas, seguido pelo estresse salino e estresse hídrico.Termos para indexação: metabolismo antioxidativo, estresse hídrico, estresse salino, estresse térmico, mulungu.1

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Section: Resultsmentioning

confidence: 99%

Activity of antioxidant enzymes and proline accumulation in Erythrina velutina Willd. seeds subjected to abiotic stresses during germination

Ribeiro

Matias

Pelacani³

et al. 2014

J. Seed Sci.

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“…Por outro lado, sabe-se que o mesotrione pode persistir no solo até 144 dias após a aplicação (Dyson et al, 2002;Chaabane et al, 2008), e a exposição permanente das células das raízes a tais concentrações residuais pode ser responsável pela permanência das mudanças dos perfis eletroforéticos dos tecidos radiculares mesmo transcorridos 7 DAA. Essa possibilidade amplia o potencial da análise do perfil eletroforético para verificação de níveis de fitointoxicação de tecidos específicos, mesmo quando outros indicadores fisiológicos indicam que a planta como um todo já se apresenta desintoxicada.…”

Section: Resultsunclassified

Análise do perfil eletroforético de proteínas citoplasmáticas para verificação do processo de desintoxicação do herbicida mesotrione em plantas de Zea mays

et al. 2014

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RESUMO -As plantas tolerantes a herbicidas apresentam rotas bioquímicas eficientes na desintoxicação dessas moléculas no interior da célula, e muitas enzimas citoplasmáticas participam desse processo. No presente trabalho, o perfil eletroforético de proteínas citoplasmáticas foi avaliado em folhas, caules e raízes de plantas de milho, durante o processo de desintoxicação, após tratamento com o herbicida mesotrione. Aos 15 dias após o plantio, foram aplicados 192 gramas por hectare (g ha -1) do mesotrione, em pós-emergência; três e sete dias após a aplicação (DAA), foram coletados os tecidos para a realização de fracionamento celular e isolamento das proteínas solúveis do citoplasma. A atividade fotossintética foi analisada como marcador fisiológico do nível de fitointoxicação em diferentes estádios (1, 2, 3, 5 e 7 DAA). Enquanto a fotossíntese foi inibida nos primeiros 3 DAA, não se observou alteração significativa a partir do quinto dia. Medidas biométricas foram realizadas aos 7 DAA, não apresentando diferenças significativas. A análise dos perfis eletroforéticos das proteínas citoplasmáticas indicou maior expressão proteica em regiões de baixa massa molecular (~ de 21 a 65 kDa) nos tecidos de folhas e caules aos 3 DAA do mesotrione. Contudo, aos sete dias observou-se recuperação de perfis semelhantes aos tecidos de plantas não tratadas com o herbicida. Nas raízes, houve redução na biossíntese de proteínas sob tratamento com herbicida, tanto aos 3 quanto aos 7 DAA. Os resultados sugerem que as alterações do perfil eletroforético das proteínas citoplasmáticas das plantas de milho refletem bem o estádio de desintoxicação de seus tecidos e que, mesmo após o processo haver se estabelecido na parte aérea, as raízes continuaram a apresentar alterações, que indicam um processo mais prolongado de desintoxicação do mesotrione sobre o sistema radicular.Palavras-chave: fitotoxicidade, milho, proteína, controle de plantas daninhas, metabolização de herbicida. ABSTRACT -Herbicide-tolerant plants have efficient biochemical pathways for detoxification of these molecules within the cell, with many cytoplasmic enzymes being involved in this process. In this study, the electrophoretic profiles of cytoplasmic proteins were evaluated in the leaves, stems, and roots of corn plants during the detoxification process after treatment with the herbicide mesotrione. At 15 days after planting, 192 grams per hectare (g ha -1) of mesotrione were administered postemergence. Three or seven days after application (DAA), tissues were collected to perform cell fractionation and isolation of soluble proteins from the cytoplasm. Photosynthetic activity was analyzed as a physiological marker of the level of intoxication at different stages (1, 2, 3, 5, and 7 DAA). While photosynthesis was inhibited in the first three days after spraying, there was no significant change on the fifth day. Biometric measurements were performed at seven DAA, and no significant differences were observed. Analysis of electrophoretic profiles of cytoplasmic proteins indi...

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“…These two cases are SNP M232 that is associated with GYDR and is located 85k bp close to the gene; and Zm00001d027539 that belongs to the glutathione S-transferase (GST) gene family (GST, EC 2.5.1.18). This second gene family plays an important role in plant response to environmental conditions ( McGonigle et al, 2002 ) plus some abiotic stresses such as herbicide ( Cataneo et al, 2003 ), heat ( Cetinkaya et al, 2014 ), and drought ( Xu et al, 2015 ; Min et al, 2016 ). In Arabidopsis, plants with mutated GST gene were more tolerant to drought stress than wild-type plants ( Chen et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Introgression of Maize Diversity for Drought Tolerance: Subtropical Maize Landraces as Source of New Positive Variants

et al. 2021

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Current climate change models predict an increased frequency and intensity of drought for much of the developing world within the next 30 years. These events will negatively affect maize yields, potentially leading to economic and social instability in many smallholder farming communities. Knowledge about the genetic resources available for traits related to drought tolerance has great importance in developing breeding program strategies. The aim of this research was to study a maize landrace introgression panel to identify chromosomal regions associated with a drought tolerance index. For that, we performed Genome-Wide Association Study (GWAS) on 1326 landrace progenies developed by the CIMMYT Genetic Resources Program, originating from 20 landraces populations collected in arid regions. Phenotypic data were obtained from early testcross trials conducted in three sites and two contrasting irrigation environments, full irrigation (well-watered) and reduced irrigation (drought). The populations were genotyped using the DArTSeq® platform, and a final set of 5,695 SNPs markers was used. The genotypic values were estimated using spatial adjustment in a two-stage analysis. First, we performed the individual analysis for each site/irrigation treatment combination. The best linear unbiased estimates (BLUEs) were used to calculate the Harmonic Mean of Relative Performance (HMRP) as a drought tolerance index for each testcross. The second stage was a joint analysis, which was performed using the HMRP to obtain the best linear unbiased predictions (BLUPs) of the index for each genotype. Then, GWAS was performed to determine the marker-index associations and the marker-Grain Yield (GY) associations for the two irrigation treatments. We detected two significant markers associated with the drought-tolerance index, four associated with GY in drought condition, and other four associated with GY in irrigated conditions each. Although each of these markers explained less than 0.1% of the phenotypic variation for the index and GY, we found two genes likely related to the plant response to drought stress. For these markers, alleles from landraces provide a slightly higher yield under drought conditions. Our results indicate that the positive diversity delivered by landraces are still present on the backcrosses and this is a potential breeding strategy for improving maize for drought tolerance and for trait introgression bringing new superior allelic diversity from landraces to breeding populations.

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Atividade de glutationa S-transferase na degradação do herbicida glyphosate em plantas de milho (Zea mays)

Cited by 20 publications

References 22 publications

Activity of antioxidant enzymes and proline accumulation in Erythrina velutina Willd. seeds subjected to abiotic stresses during germination

Activity of antioxidant enzymes and proline accumulation in Erythrina velutina Willd. seeds subjected to abiotic stresses during germination

Análise do perfil eletroforético de proteínas citoplasmáticas para verificação do processo de desintoxicação do herbicida mesotrione em plantas de Zea mays

Introgression of Maize Diversity for Drought Tolerance: Subtropical Maize Landraces as Source of New Positive Variants

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