Adição de antioxidantes ao sêmen de carneiros e seus efeitos após a descongelação

Abstract

Aimed to evaluate the effect of adding antioxidants as ascorbic acid, melatonin and Trolox C to diluted semen of ram with oxidative stress to potenciate fertilization after cryopreservation. Ten samples collected were diluted in Tris-egg yolk to a final concentration of 200x10 6 sperm/mL and kept in a water bath at 32°C. Antioxidants were added as follows: 100µM melatonin (MEL) +.05% ascorbic acid (AA); 100µM of MEL + 90µL of Trolox C (TRO); 90µL of TRO + 0.05% AA; and 100µM of MEL0.05% AA + 90µL of TRO. Semen was cooled in a cold chamber at 5°C for two hours and packaged, sealed in 0.5mL straws, packaged under liquid nitrogen vapor (N 2 L), 8cm of water depth for 15 minutes, and then immersed in N 2 L. Samples were assayed for motility, integrity of the plasma membrane and acrosomal membrane, mitochondrial activity, binding assay and oxidative stress spermatozoa. The variables were analyzed by ANOVA and means compared by Tukey test (P<0.05). Percentage of total and progressive motility was higher for sperm treated with MEL+AA+TRO (67% and 49.89%), MEL+AA (64.37% and 45.61%) and MEL+TRO (61.65% and 41.15%) compared with the other treatments (P<0.05). The integrity of the plasma membrane and acrosome was higher for all semen treated with antioxidant associations compared with control (P<0.05). Mitochondrial activity was higher in sperm treated with MEL+AA+TRO compared all treatments (P<0.05). The number of sperm binding to perivitelline membrane was higher for semen treated with antioxidant associations compared with control; also sperm treated with MEL+AA+TRO demonstrated higher effect of all (P<0.05). No difference was observed between the treatments by oxidative stress sperm (P>0.05). The addition of melatonin, ascorbic acid and Trolox C in diluted semen of ram improves sperm quality after thawing.

The use of açaí as a potential antioxidant for bovine semen cryopreservation

“…Sources of lipoproteins or high molecule weight compounds are added to diluents to prevent thermal shock, and the addition of glucose and fructose as energy sources. Moreover, there are also cryoprotectants and antioxidants in its composition (Oliveira et al, 2013;Souza et al, 2017).…”

“…For this reason, it becomes necessary to promote an additional protection additional during semen cryopreservation. This additional protection can be accomplished by adding substances with antioxidant activity to diluents (Souza et al, 2017).…”

Bioprospecção do extrato de bromelina acrescida de ACP® na qualidade do sêmen caprino

“…Nesse contexto, o desenvolvimento de diluidores seminais que mantêm a viabilidade do sêmen, é de suma importância na reprodução de pequenos ruminantes, uma vez que contribui significativamente na redução de perdas durante o processo de criopreservação por crioinjúrias. E, atualmente, estudos vêm sendo desenvolvidos objetivando a adição de substâncias aos meios diluentes, a fim de proporcionar maior proteção às células espermáticas durante o processo de criopreservação de sêmen (Souza et al, 2017).…”