Isolamento e caracterização de espécies de Pythium de ambientes aquáticos no Estado do Rio Grande do Sul e avaliação da patogenicidade em modelo experimental

Zambrano, Cristina Gomes; Fonseca, Anelise O.S.; Valente, Júlia de Souza Silveira; Braga, Caroline Quintana; Sallis, Eliza Simone Viégas; Azevedo, María Isabel de; Weiblen, Carla; Santúrio, Jânio Morais; Botton, Sônia de Ávila; Pereira, Daniela Isabel Brayer

doi:10.1590/s0100-736x2017000500006

Cited by 10 publications

(9 citation statements)

References 31 publications

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“…In nature, P. insidiosum is observed in two forms: mycelium and zoospore (an infective unit) [2]. Several groups of investigators have successfully isolated P. insidiosum from swampy areas in Australia, Thailand, the United States, and Brazil [3–6]. While most pathogenic Pythium species infects plants, P. insidiosum infects humans and animals, and causes the life-threatening disease, called pythosis [7].…”

Section: Objectivementioning

confidence: 99%

Data on whole genome sequencing of the oomycete Pythium insidiosum strain CBS 101555 from a horse with pythiosis in Brazil

et al. 2018

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ObjectivesThe oomycete Pythium insidiosum infects humans and animals worldwide, and causes the life-threatening condition, called pythosis. Most patients lose infected organs or die from the disease. Comparative genomic analyses of different P. insidiosum strains could provide new insights into its pathobiology, and can lead to discovery of an effective treatment method. Several draft genomes of P. insidiosum are publicly available: three from Asia (Thailand), and one each from North (the United States) and Central (Costa Rica) Americas. We report another draft genome of P. insidiosum isolated from South America (Brazil), to serve as a resource for comprehensive genomic studies.Data descriptionIn this study, we report genome sequence of the P. insidiosum strain CBS 101555, isolated from a horse with pythiosis in Brazil. One paired-end (180-bp insert) library of processed genomic DNA was prepared for Illumina HiSeq 2500-based sequencing. Assembly of raw reads provided genome size of 48.9 Mb, comprising 60,602 contigs. A total of 23,254 genes were predicted and classified into 18,305 homologous gene clusters. Compared with the reference genome (the P. insidiosum strain Pi-S), 1,475,337 sequence variants (SNPs and INDELs) were identified in the organism. The genome sequence data has been deposited in DDBJ under the accession numbers BCFP01000001–BCFP01060602.

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Section: Objectivementioning

confidence: 99%

Data on whole genome sequencing of the oomycete Pythium insidiosum strain CBS 101555 from a horse with pythiosis in Brazil

et al. 2018

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“…P. insidiosum can be isolated from swampy areas in several countries across the world (i.e., Thailand, Australia, the United States, and Brazil) [ 34 , 35 , 36 , 37 , 38 , 39 ]. Recently, Jara et al successfully isolated P. insidiosum throughout the study area in the Chincoteague National Wildlife Refuge in Virginia, the United States [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Several investigators can isolate P. insidiosum from agricultural or non-residential areas in northern Thailand, Australia, the United States, and Brazil [ 34 , 35 , 36 , 37 , 38 , 39 ]. To obtain a complete picture of how the pathogen circulates in the environment, we aim to survey the presence of P. insidiosum in a crowded city like Bangkok, as well as rural areas of central and southern Thailand.…”

Section: Introductionmentioning

confidence: 99%

Identification and Biotyping of Pythium insidiosum Isolated from Urban and Rural Areas of Thailand by Multiplex PCR, DNA Barcode, and Proteomic Analyses

Htun

Laikul

Pathomsakulwong

et al. 2021

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Pythium insidiosum causes pythiosis, a fatal infectious disease of humans and animals worldwide. Prompt diagnosis and treatment are essential to improve the clinical outcome of pythiosis. Diagnosis of P. insidiosum relies on immunological, molecular, and proteomic assays. The main treatment of pythiosis aims to surgically remove all affected tissue to prevent recurrent infection. Due to the marked increase in case reports, pythiosis has become a public health concern. Thailand is an endemic area of human pythiosis. To obtain a complete picture of how the pathogen circulates in the environment, we surveyed the presence of P. insidiosum in urban (Bangkok) and rural areas of Thailand. We employed the hair-baiting technique to screen for P. insidiosum in 500 water samples. Twenty-seven culture-positive samples were identified as P. insidiosum by multiplex PCR, multi-DNA barcode (rDNA, cox1, cox2), and mass spectrometric analyses. These environmental strains of P. insidiosum fell into Clade-II and -III genotypes and exhibited a close phylogenetic/proteomic relationship with Thai clinical strains. Biodiversity of the environmental strains also existed in a local habitat. In conclusion, P. insidiosum is widespread in Thailand. A better understanding of the ecological niche of P. insidiosum could lead to the effective prevention and control of this pathogen.

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“…Nine scientific articles described the successful isolations and molecular detections of P. insidiosum in water or soil samples from the environment in four countries (i.e., U.S.A., Brazil, Thailand, and Australia; as indicated by the stars in Figure 3) [4,[68][69][70][71][72][73][74][75]. P. insidiosum was first isolated from swampy areas in Queensland, Australia [4].…”

Section: Ecological Distribution Of P Insidiosummentioning

confidence: 99%

Global Distribution and Clinical Features of Pythiosis in Humans and Animals

Yolanda

Krajaejun

2022

JoF

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Pythiosis is a difficult-to-treat infectious disease caused by Pythium insidiosum. The condition is unfamiliar among healthcare workers. Manifestation of pythiosis is similar to other fungal infections, leading to misdiagnosis and delayed treatment. The geographical extent of pythiosis at a global scale is unclear. This study aimed to analyze the clinical information recorded in the scientific literature to comprehensively project epidemiological characteristics, clinical features, and future trends of pythiosis. From 1980 to 2021, 4203 cases of pythiosis in humans (n = 771; 18.3%) and animals (primarily horse, dog, and cow; n = 3432; 81.7%), with an average of 103 cases/year, were recruited. Pythiosis case reports significantly increased in the last decade. Pythiosis spanned 23 tropical, subtropical, and temperate countries worldwide. Some patients acquired pythiosis from a trip to an endemic country. Strikingly, 94.3% of human cases were in India and Thailand, while 79.2% of affected animals were in the U.S.A. and Brazil. Clinical features of pythiosis varied. Vascular and ocular pythiosis were only observed in humans, whereas cutaneous/subcutaneous and gastrointestinal infections were predominant in animals. Mortality depended on host species and clinical forms: for example, none in patients with ocular pythiosis, 0.7% in cows with a cutaneous lesion, 26.8% in humans with vascular disease, 86.4% in dogs with gastrointestinal pathology, and 100% in several animals with disseminated infection. In summary, this study reports up-to-date epidemiological and clinical features of pythiosis in humans and animals. It increases awareness of this life-threatening disease, as the illness or outbreak can exist in any country, not limited to the endemic areas.

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Isolamento e caracterização de espécies de Pythium de ambientes aquáticos no Estado do Rio Grande do Sul e avaliação da patogenicidade em modelo experimental

Cited by 10 publications

References 31 publications

Data on whole genome sequencing of the oomycete Pythium insidiosum strain CBS 101555 from a horse with pythiosis in Brazil

Data on whole genome sequencing of the oomycete Pythium insidiosum strain CBS 101555 from a horse with pythiosis in Brazil

Identification and Biotyping of Pythium insidiosum Isolated from Urban and Rural Areas of Thailand by Multiplex PCR, DNA Barcode, and Proteomic Analyses

Global Distribution and Clinical Features of Pythiosis in Humans and Animals

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