In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

Moura, Jusciene Bagagi; Vargas, Agueda Castagna de; Gouveia, Gisele Veneroni; Gouveia, João José de Simoni; Ramos-Júnior, Juracy C.; Botton, Sônia de Ávila; Pereira, Eugênia C.; Costa, Mateus Matiuzzi da

doi:10.1590/s0100-736x2017000400011

Cited by 10 publications

(4 citation statements)

References 65 publications

(81 reference statements)

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“…It is present in different species of lichens as a product of the secondary metabolism. A series of in vitro studies demonstrated not only anti-inflammatory, antioxidant, antitumor, and antiviral properties, but also a potent antibacterial activity against mycobacteria and several Gram-positive and Gram-negative bacteria [15][16][17][18]. Along with these data, the interest in this substance has risen since it could be used as a potential antimicrobial compound.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa

Júnior

Silva

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The present study aimed to characterize the susceptibility profile of Pseudomonas aeruginosa and Acinetobacter spp. clinical isolates to polymyxin B in a public hospital in Recife-PE, Brazil, between the years of 2018 and 2019, as well as to search for the presence of the mcr-1 gene and evaluate the interaction between polymyxin B and usnic acid against these isolates. The strains were identified using the BD Phoenix™ automated system and the agar-spot test was used to determine the susceptibility profile to polymyxin B. The minimum inhibitory concentrations (MICs) of usnic acid and polymyxin B were determined through the broth microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). Subsequently, Polymerase Chain Reaction (PCR) was performed to detect the mcr-1 gene in the isolates. The interaction between usnic acid and polymyxin B was evaluated by the Checkerboard assay. Among 34 isolates of P. aeruginosa, 26.5% (9/34) were positive for the polymyxin B agar-spot test, and 11.8% (4/34) presented an intermediate susceptibility (MIC = 4 μg/mL), while 14.7% (5/34) presented antimicrobial resistance with MIC values ranging from 8 to 32 μg/mL. Among 38 isolates of Acinetobacter spp., 13.2% (5/38) were positive for the polymyxin B agar-spot test and all of them were resistant to polymyxin B with a MIC value > 32 μg/mL. The mcr-1 gene was not detected in the clinical isolates. Regarding usnic acid, it presented a moderate antibacterial activity against two P. aeruginosa isolates (MIC = 250 μg/mL) and no activity was detected against the others. A synergistic effect between usnic acid and polymyxin B was observed against three clinical isolates of P. aeruginosa which were resistant to polymyxin B (FICI ≤ 0.5). Therefore, it was possible to observe that usnic acid is a promising candidate to be used in combination with polymyxin B against infections caused by resistant P. aeruginosa.

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Section: Introductionmentioning

confidence: 99%

Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa

Júnior

Silva

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…Estudos sobre a atividade antimicrobiana de substâncias liquênicas normalmente relatam a maior sensibilidade de bactérias Gram-positivas a extratos de diferentes espécies de fungos liquenizados 13,14,15,16,17 . Concomitante a isso, alguns autores reportaram a ineficácia dessas substâncias contra cepas de bactérias Gram-negativas 18,19 .…”

Section: Discussionunclassified

Atividade antimicrobiana de fungos liquenizados ocorrentes no campus Belém da Universidade Federal do Pará, estado do Pará, Brasil

Vieira

Pinheiro

et al. 2019

Revista Pan-Amazônica de Saúde

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Avaliar a atividade antimicrobiana de extratos obtidos a partir de fungos liquenizados ocorrentes no campus Belém da Universidade Federal do Pará, Brasil. MATERIAIS E MÉTODOS: Amostras liquênicas de Leptogium sp. e Parmotrema sp. foram submetidas à extração orgânica por esgotamento a frio, seguindo a série eluotrópica clorofórmio e acetona. Os extratos obtidos foram testados frente a cepas de bactérias Gram-positivas, Gram-negativas e álcool-ácido resistentes, fungos filamentosos e leveduras, empregando-se o método de disco difusão em ágar. Discos de papel foram impregnados com 20 µL de cada extrato na concentração de 4 mg/mL e depositados sobre o meio previamente inoculado com os microrganismos teste. Os resultados foram avaliados pelo diâmetro dos halos de inibição em torno dos discos. Em seguida, os extratos foram submetidos à cromatografia ascendente em camada delgada e a ensaios bioautográficos para detecção dos princípios ativos. RESULTADOS: Houve maior potencial antimicrobiano dos extratos de Parmotrema sp. com halos de inibição que variaram de 8 a 12 mm para bactérias e de 8 a 21 mm para fungos. As bactérias Escherichia coli, Klebsiella pneumoniae e Mycobacterium phlei apresentaram maior sensibilidade, pois foram inibidas pelas duas espécies liquênicas. Em relação aos fungos, os mais sensíveis foram Trichophyton tonsurans, Microsporum gypseum e Epidermophyton floccosum. CONCLUSÃO: Este trabalho ampliou o conhecimento sobre fungos liquenizados da Região Amazônica e possibilitou a obtenção de substâncias antimicrobianas viáveis para a inibição do crescimento de bactérias e fungos de importância médica.

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“…Atranorin (isolated from Physcia aipolia), fumarprotocetraric acid (Cladonia furcata), gyrophoric acid (Umbilicaria polyphylla), lecanoric acid (Ochrolechia androgyna), physodic acid (Hypogymnia physodes), protocetraric acid [Flavoparmelia caperata (as Parmelia caperata)], stictic acid [Xanthoparmelia conspersa (as Parmelia conspersa)] and usnic acid (Flavoparmelia caperata) exhibited potential antimicrobial effects against six types of bacteria and ten types of fungi, including human, animal, plant pathogens, mycotoxin producers and food-spoilage organisms (Branislav and Marijana, 2008). Usnic acid was found to be a good antimicrobial agent when compared to streptomycin (Moura et al 2017). The depsidone molecules of physodic acid and stictic acid were identified as the weakest.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Mycorrhiza and Lichens as Two Models of Fungal Symbiosis

Elkhateeb¹,

Somasekhar²,

Thomas³

et al. 2021

J microb biotech food sci

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Fungi have evolved many symbioses including different eukaryotes and prokaryotes. Mutualism is one of the symbioses and here both symbionts benefit from the interaction. The most common mutualistic relationships involving fungi are mycorrhiza and lichens. A mycorrhiza is a symbiotic relationship between a roots of a plant and a fungus while lichen associates between a fungus and an algae. Many studies have performed to investigate these symbiotic relationships in depth, however, still have some debates on them, though many taxonomists rely on genetic analyses besides with traditional morphological data. In our study, it highlights the nature, importance, nutritional and pharmaceutical uses, and applications of these mysterious dual between fungi and plant and/or algae.

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In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

Cited by 10 publications

References 65 publications

Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa

Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa

Atividade antimicrobiana de fungos liquenizados ocorrentes no campus Belém da Universidade Federal do Pará, estado do Pará, Brasil

Mycorrhiza and Lichens as Two Models of Fungal Symbiosis

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