Prevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma

Abstract

RESUMO.-[Prevalência e suscetibilidade in vitro deIn order to assess the prevalence of Methicillin-resistant Staphylococcus pseudintermedius from skin and nostrils of dogs with pyoderma, to determine its in vitro susceptibility, and to correlate these data with the presence of the mecA gene, 43 dogs were selected. Samples were collected from secretion of their skin lesions and right nostril, cultured, and analyzed for phenotypic and genotypic characteristics of the bacteria studied. In 62 samples (91%) the microorganism was classified as S. pseudintermedius. The rate of resistance against antibiotics ranged from 7% (amikacin; 4/62) to 77% (sulfamethoxazole + trimethoprim; 48/62). Resistance against oxacillin was found in 34% of the samples (21/62). Twenty-five samples (37%) were strains that carried the mecA gene. A significant correlation (P<0.01) was found between presence of the mecA gene and oxacillin resistance. Seventeen dogs were mecA gene carriers, and 8 (47%) of them had the gene in the skin lesions and nostril. A significant correlation (P<0.01) was also observed between the presence of mecA gene in the skin lesions and nostrils. Oxacillin resistance in vitro can be safely used to indicate the presence of mecA gene in MRSP samples. The nostrils can be a reservoir of MRSP in dogs. observada entre a presença do gene mecA nas lesões de pele e nas narinas. Sendo assim, resistência à oxacilina in vitro pode ser aferida com segurança para indicar a presença do gene mecA em amostras de MRSP, e as narinas podem constituir em um reservatório dos microorganismos em cães.

Antibiotic Resistance Profile and Biofilm Production of Staphylococcus pseudintermedius Isolated from Dogs in Thailand

“…Our study showed that MRSP was detected at 28.30%, similar to Kadlec et al, who recorded 28% MRSP isolated from dogs admitted at a small animal hospital between July 2006 and April 2013 [ 7 ]. Moreover, the Department of Dermatology at the Veterinary Hospital of Universidade Federal de Minas Gerais (Belo Horizonte, Brazil) indicated 29.55%, similar to a study at the Department of Veterinary Medicine and Animal Production, University of Naples (Naples, Italy) indicating 30.17% [ 23 , 24 ].…”

Virulence Genes Detection and Antimicrobial Susceptibility of Staphylococcus pseudintermedius Isolates from Canine Skin Infection in Chennai, India

“…O gene mecA foi encontrado em todos os 22 isolados (100%) fenotipicamente resistentes à meticilina e não era carreado por isolados sensíveis. Tal observação é corroborada por dados semelhantes reportados em outros estudos (BEMIS et al, 2006;BOTONI et al, 2016;WORTHING et al, 2018), o que aponta para uma expressiva correlação fenogenotípica nessa espécie, sugerindo que em cepas MRSP não existem variantes gênicas que impliquem na não-detecção do gene mecA. Tal hipótese é suportada pela não-detecção da variante ao utilizar os iniciadores desenvolvidos por Melo e colaboradores (2014).…”

Determination of the frequency, species distribution and antimicrobial resistance of staphylococci isolated from dogs and their owners in Trinidad

“…Oxacillin-resistant isolates of the SIG were detected in 4.5% of humans and 12.5% of dogs, indicative of methicillin-resistant S. pseudintermedius (MRSP). A wide range of MRSP prevalence rates have been reported in dogs, from as low as 2.6% in Norway [73], to as high as 34% in Brazil [74] and 40.5% in Canada [75]. The variations in the prevalence values may be due to the sites sampled as well as the methodology used to isolate the staphylococci and determine methicillin resistance.…”