Identification and characterization of Aspergillus fumigatus isolates from broilers

Spanamberg, Andréia; Ferreiro, Laerte; Machado, Gustavo; Fraga, Cibele Floriano; Araújo, Ricardo

doi:10.1590/s0100-736x2016000700005

Cited by 10 publications

(8 citation statements)

References 24 publications

(26 reference statements)

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“…O A. fumigatus já foi isolado de frangos de corte e de galinhas de diversas origens com histórico de problemas respiratórios no México com uma frequência de 9,58% (OCA et al, 2017), ocorrendo ◁◀ ◁◀ principalmente naquelas aves oriundas do mercado local com idade variando de 3 dias a 4 semanas. Em aves saudáveis em abatedouro no Estado do Rio Grande do Sul, Spanamberg et al (2016) observaram que 9,5% das amostras foram positivas para o isolamento de fungos Aspergillus fumigatus.…”

Section: Resultsunclassified

Aerossaculite fúngica em frangos de corte

Lima Neto,

Silva,

Olinda

et al. 2023

RBM

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Este trabalho tem o objetivo de relatar um caso de aspergilose em frangos de corte com 49 dias de idade. O caso ocorreu em uma granja comercial de frangos de corte de múltiplas idades localizadas na região metropolitana de Fortaleza. Problemas respiratórios, pouco responsivos a antibióticos, foram observados em frangos de corte durante o período de criação. Diante da elevada morbidade e mortalidade, aves foram tomadas para necropsia, 10 aves com morte recente e 5 aves apresentando sintomas respiratórios foram sacrificadas. Na necropsia observou-se corrimento e área focal nodular amarelada na cavidade nasal, opacidade e espessamento de sacos aéreos e presença de material caseoso amarelado. O exame histopatológico revelou uma infiltração multifocal de histiócitos e heterófilos na submucosa, além de focos de necrose do epitélio. Nos sacos aéreos observou-se focos de infiltração histiocitária, alguns associados com área central de necrose contendo imagens negativas de hifas (compatíveis com Aspergillus sp.). Diante da associação do quadro respiratório das aves aos achados de necropsia e histopatológico, diagnosticou-se o quadro de aerossaculite granulomatosa associada a hifas fúngicas, sugestivo de aspergilose.

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Section: Resultsunclassified

Aerossaculite fúngica em frangos de corte

Lima Neto,

Silva,

Olinda

et al. 2023

RBM

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“…The Qiagen DNeasy  plant mini DNA extraction kit (Qiagen, Hilden, Germany) protocol was used to extract DNA from the Aspergillus conidia according to manufacturer instructions. Molecular identification was performed using specific primers for identification of Aspergillus section Fumigati and A. fumigatus (Spanamberg et al 2016). Multiplex PCR amplification was performed a total volume of 25μL containing 1μL of DNA extract, 12.5μL Qiagen Taq PCR master mix (Qiagen, Hilden, Germany) and 0.5μL of each primer (for a 0.2μM final concentration of each primer).…”

Section: Aspergillus Isolates and Molecular Identification A Total Omentioning

confidence: 99%

Antifungal susceptibility profile of Aspergillus fumigatus isolates from avian lungs

et al. 2020

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ISSN 0100-736X (Print) ISSN 1678-5150 (Online) RESUMO.-[Perfil de suscetibilidade antifúngica de isolados de Aspergillus fumigatus provenientes de pulmões de aves.] Os testes de suscetibilidade são essenciais para informar o correto manejo das infecções por Aspergillus. Neste estudo apresentamos o perfil antifúngico de isolados de A. fumigatus provenientes de pulmões de aves com e sem aspergilose. Cinqüenta e três isolados foram testados quanto à susceptibilidade antifúngica ao voriconazol (VRC), itraconazol (ITZ), anfotericina B (AMB) e caspofungina (CSP) pelo método de referência de microdiluição do caldo M38-A2. Cinco isolados foram resistentes a mais de um antifúngico (CSP + AMB, VRC + ITZ e AMB + ITZ). Quinze (28%) isolados suscetíveis -com exposição aumentada (I) ao ITZ foram sensíveis ao VRC. A resistência ao AMB (>2μg/mL) foi observada em apenas quatro isolados. Onze (21%) A. fumigatus apresentaram resistência a ITZ (13%) e VRC (8%). O isolamento de fungos de amostras respiratórias tem sido considerado de utilidade limitada no diagnóstico ante mortem de aspergilose em aves. No entanto, os resultados sugerem que a detecção e o perfil de suscetibilidade a antifúngicos podem ser úteis para o monitoramento da terapia de espécies aviárias, assim como a emergência da resistência antifúngica e quais condições podem estar associadas ao evento. ABSTRACT.-Spanamberg A., Ravazzolo A.P., Denardi L.B., Hartz S.A., Santurio J.M., Driemeier D. & Ferreiro L. 2020. Antifungal susceptibility profile of Aspergillus fumigatus isolates from avian lungs. Pesquisa Veterinária Brasileira 40(2):102-106. Susceptibility testing is essential to inform the correct management of Aspergillus infections.In this study we present antifungal susceptibility profile of A. fumigatus isolates recovered from lungs of birds with and without aspergillosis. Fifty three isolates were tested for their antifungal susceptibility to voriconazole (VRC), itraconazole (ITZ), amphotericin (AMB) and caspofungin (CSP) using the M38-A2 broth microdilution reference method. Five isolates were resistant to more than one antifungal drug (CSP + AMB, VRC + ITZ and AMB + ITZ). Fifteen (28%) isolates with susceptible increased exposure (I) to ITZ were sensible to VRC. Resistance to AMB (>2μg/mL) was observed in only four isolates. Eleven (21%) A. fumigatus present resistance to ITZ (13%) and VRC (8%). Fungal isolation from respiratory samples has been regarded as being of limited usefulness in the ante mortem diagnosis of aspergillosis in birds. However, the results suggest that the detection and antifungal susceptibility profile may be helpful for monitoring of therapy for avian species and where antifungal resistance might be emerging and what conditions are associated to the event.

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“…DNA is extracted directly from the clinical samples for PCR testing of human blood, plasma, and serum. In contrast, in birds, most PCR tests are on samples from cultured isolates, which introduces additional steps and increases the amount of time needed for analyses. Therefore, future PCR testing in birds and mammals should adopt similar protocols to those used in human samples (ie, extracting fungal DNA directly from biological fluids) …”

Section: Molecularmentioning

confidence: 99%

“…In contrast to the extensive reliability, specificity, and sensitivity tests performed on PCR for aspergillosis diagnoses in humans, the method is in its infancy in veterinary medicine. Nevertheless, PCR has been tested on cultured isolates from psittacines, falcons, ostriches, penguins, pigeons, chickens, and white storks; however, these tests are still mostly in the research stages. PCR analysis and identification of micro‐isolated fungal hyphae from avian lung biopsies has been tested .…”

Section: Molecularmentioning

confidence: 99%

The current status of avian aspergillosis diagnoses: Veterinary practice to novel research avenues

Savelieff¹,

Pappalardo

Azmanis

2018

Veterinary Clinical Pathol

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Aspergillus fungal infections continue to be a significant cause of morbidity and mortality in birds that can, in part, be attributed to the lack of a diagnostic "gold standard" for Aspergillus infection, and which delays the diagnosis, treatment, and outcome of avian patients. At present, none of the available methods in veterinary care can detect aspergillosis early enough and with the accuracy, precision, and specificity required of an ideal diagnostic tool. Therefore, researching methods of Aspergillus detection is still an active area of inquiry, and novel techniques continue to emerge. This review will provide a brief overview of current clinical methods, with an emphasis on avian care, in addition to a series of techniques in development that could offer distinct advantages over existing methods.

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Identification and characterization of Aspergillus fumigatus isolates from broilers

Cited by 10 publications

References 24 publications

Aerossaculite fúngica em frangos de corte

Aerossaculite fúngica em frangos de corte

Antifungal susceptibility profile of Aspergillus fumigatus isolates from avian lungs

The current status of avian aspergillosis diagnoses: Veterinary practice to novel research avenues

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