Influence of scrotal bipartition on spermatogenesis yield and sertoli cell efficiency in sheep

Abstract

RESUMO.-[Influência da bipartição escrotal sobre o rendimento da espermatogênese e eficiência das cé-lulas de Sertoli em ovinos.] Com o objetivo de avaliar o efeito da bipartição escrotal sobre a espermatogênese em ovinos, foram utilizados os testículos de 12 ovinos sem raça definida oriundos de criadouros do município de Patos-PB, Brasil, distribuídos em dois grupos, GI de seis animais com bipartição escrotal e o GII de seis animais sem bipartição escrotal. Realizou-se a aferição da biometria testicular, em seguida, os testículos foram coletados, fixados em Bouin e fragmentos foram processados para obtenção de lâmi-nas histológicas. Foi estimado o rendimento da espermatogênese e eficiência das células de Sertoli contando-se as células da linhagem espermatogênica no estádio I do Ciclo do Epitélio Seminífero, bem como as células de Sertoli. Os resultados foram submetidos à análise de variância pelo programa ASSISTAT v.7.6 e os valores médios foram comparados pelo teste Student-Newman-Keuls (SNK) a 5% de significância. Os parâmetros de biometria testicular não apresentaram diferença estatística (p>0,05) entre os grupos. Os rendimentos meiótico, espermatogênico e a eficiên-cia das células de Sertoli mostraram-se superiores em animais bipartidos (p<0,05), enquanto o rendimento mitótico não diferiu (p>0,05) entre GI e GII. With the objective to assess the effect of scrotal bipartition on spermatogenesis in sheep, the testes were used from 12 crossbred rams of sheep farms in the municipality of Patos, Paraíba, Brazil, distributed into two groups: GI with six rams with scrotal bipartition, and GII with six rams without scrotal bipartition. The testicular biometry was measured and the testes were collected, fixed in Bouin and fragments were processed to obtain histological slides. The spermatogenesis yield and the Sertoli cell efficiency was estimated by counting the cells of the spermatogenetic line at stage one of the seminiferous epithelium cycle and the Sertoli cells. The results were submitted to analysis of variance with the AS-SISTAT v.7.6 program and the mean values were compared by the Student-Newman-Keuls test (SNK) at 5% significance. The testicular biometric parameters did not show statistical difference (p>0.05) between the groups. The meiotic, spermatogenetic and Sertoli cell efficiency were higher in bipartitioned rams (p<0.05), while the mitotic yield did not differ (p>0.05) between GI and GII. The results indicated that there is superiority in the spermatogenetic parameters of bi-partitioned rams, suggesting that these sheep present, as reported in goats, indication of better reproductive indices.

Diagnosis and Customs Revealed by Peasants and Shepherds during the Transhumant Grazing of Pastoreña Goats in the Mixteca of Oaxaca, Mexico

“…Later in the mating periods, one buck is selected for every 40 females, meeting the following characteristics: (a) Age from 2 to 3 years, (b) Males with good body conformation, (c) white or creamy coverage, (d) Large testes with the presence of a longitudinal bipartition in the middle of the scrotum. The herders believe that this last selected physical characteristic is associated with higher sperm production and quality [58,59]. They also believe that the offspring-female descendants of the selected bucks, during adulthood, may have more twin kids, well-conformed udders, and better milk production [22].…”

Pengaruh bentuk scrotal bipartition terhadap kadar follicle stimulating hormone

“…Domba dengan bentuk skrotum yang memiliki partisi (with bipartition), lebih memiliki indikasi indeks reproduksi yang lebih baik dibandingkan dengan ternak yang memiliki bentuk skrotum without bipartition. Sel meiotik, spermatogenetik, dan sel sertoli pada domba dengan bentuk skrotum with bipartition memiliki efisiensi lebih tinggi dibandingkan domba bentuk skrotum without bipartition (Rodrigues et al, 2016).…”

“…Lingkar skrotum pada kambing Kejobong ini memiliki angka yang berbeda dengan hasil yang diperoleh oleh Hendri et al (2017) yaitu lingkar skrotum pada kambing Peranakan Ettawa (PE) pada umur 1 -1,5 adalah 24,1 ± 1,5 dan pada umur 2 -4 tahun adalah 26,4 ± 0,9 cm. Menurut Rodrigues et al (2016) lingkar skrotum pada domba with bipartition adalah 25,33 ± 1,21 cm dan without bipartition adalah 25,16 ± 1,94 cm. Syamyono et al (2015) menyatakan bahwa lingkar skrotum pada kambing Kejobong muda yaitu 19,1 -25,0 cm dan kambing Kejobong dewasa 21,1 -27,0 cm.…”

“…Hasil penelitian menunjukkan bahwa lebar testis pada kambing Kejobong (Tabel 1) pada bentuk skrotum A dan B memiliki ukuran testis yang sama. Angka ini berbeda dengan hasil penelitian yang diperoleh oleh Rodrigues et al (2016) yang menyatakan bahwa lebar testis kanan pada skrotum domba with bipartition adalah 4,92 ± 0,19 cm dan yang kiri adalah 4,92 ± 0,26 cm. Lebar testis kanan pada skrotum without bipartition adalah 4,83 ± 0,17 cm dan yang kiri adalah 4,83 ± 0,17 cm.…”

“…Uji-t menunjukkan bahwa tidak ada pengaruh bentuk skrotum A maupun bentuk skrotum B (P>5%) terhadap kadar FSH (Lampiran 5). Hal ini sesuai dengan pendapat Rodrigues et al (2016)…”