Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil

Cargnelutti, Juliana Felipetto; Masuda, Eduardo Kenji; Neuls, Mariana G.; Weiblen, Rudi; Flores, Eduardo Furtado

doi:10.1590/s0100-736x2015000600012

Cited by 6 publications

(7 citation statements)

References 10 publications

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“…In Brazil, despite the wide distribution of these infections and informal reports by veterinarians, very few reports describe viral respiratory disease in dogs. 13 , 14 , 15 , 16 , 17 , 18 Additionally, there is little information regarding these infections in local environments with high densities and constant animal movement such as dog shelters. The identification of the more common respiratory viruses in dogs in various epidemiological conditions is essential for developing efficient control and prevention measures.…”

Section: Introductionmentioning

confidence: 99%

Detection of respiratory viruses in shelter dogs maintained under varying environmental conditions

Monteiro

Cargnelutti

Martins

et al. 2016

Brazilian Journal of Microbiology

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Three dog shelters in Rio Grande do Sul were investigated for associations between the occurrence of respiratory viruses and shelter environmental conditions. Nasal secretions randomly collected during the cold season were tested via PCR, and this data collection was followed by nucleotide sequencing of the amplicons. In shelter #1 (poor sanitary and nutritional conditions, high animal density and constant contact between dogs), 78% (58/74) of the nasal samples were positive, 35% (26/74) of which were in single infections and 44% (32/74) of which were in coinfections. Shelters #2 and #3 had satisfactory sanitary and nutritional conditions, outdoors exercise areas (#2) and animal clustering by groups (#3). In shelter #2, 9% (3/35) of the samples were positive for Canine parainfluenza virus (CPIV), and 6% (2/35) were positive for Canid herpesvirus 1 (CaHV-1). In shelter #3, 9% (7/77) of the samples were positive for Canine adenovirus type 2 (CAdV-2), and 1% (1/77) were positive for Canine distemper virus (CDV). The amplicon sequences (CPIV and CDV nucleoprotein gene; CAdV-2 E3 gene; CaHV-1 glycoprotein B gene) showed 94–100% nucleotide identity with GenBank sequences. Our results demonstrate that CPIV, CAdV-2 and CDV are common in dog shelters and that their frequencies appear to be related with environmental and nutritional conditions. These results indicate the need for control/prevention measures, including vaccination and environmental management, to minimize these infections and improve dog health.

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Section: Introductionmentioning

confidence: 99%

Detection of respiratory viruses in shelter dogs maintained under varying environmental conditions

Monteiro

Cargnelutti

Martins

et al. 2016

Brazilian Journal of Microbiology

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“…So, infected puppies pose a threat for other animals in the colony. Cargnelutti et al (2015) also described an outbreak of systemic fatal illness causing neonatal mortalities using histopathology, VNT and PCR [22]. In study of Larsen et al…”

Section: Discussionmentioning

confidence: 99%

The first molecular detection of Canine herpesvirus-1 in reproductive specimens of adult dogs in Iran

Rezaei

Jajarmi

Alizadeh

et al. 2019

Preprint

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Background Canine herpesvirus-1 (CHV-1) is recognized to be enzootic in the dog population with a widespread distribution. This pathogen leads to a lethal generalized illness in newborn puppies and is associated with reproductive disorders. CHV-1 should be considered as an important pathogen of neonatal death and infertility; so, it appears to pose a threat for breeding kennels. Although serologic data point to the circulation of CHV-1 among dogs of Iran, not definitive diagnosis has been conducted based on the molecular assay. So, this research was done to detect the prevalence of CHV-1 in dogs of Kerman. In this study, the presence of CHV-1 in vaginal specimens and biopsies of the uterus of dogs referring to the Veterinary Hospital of Shahid Bahonar University of Kerman was determined. Samples were collected and evaluated using real-time PCR.Results Viral DNA was detected in 21 samples from a total of 140 (15%) collected samples which were related to 14 uterine samples (20%) and 7 (10%) vaginal specimens. The association of this virus with age, breed, housing, pregnancy and reproductive disorders was not significant.Conclusions This study is the first molecular detection of CHV-1 in reproductive samples of dogs in Iran. Considering the significant prevalence of this virus, it is necessary to carry out management measures in controlling and preventing this disease. Tracing CHV-1 requires further research on this virus in dogs of this region.

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“…The gC gene is made up of 1380 bp and its nucleotide sequence is available in official databases (Genbank AF361074.1). Thus, the establishment of a conventional PCR protocol to detect gC can be obtained using some bioinformatic tools such as the In silico design of primers, since its detection through this molecular technique -apparently-has not had the same diffusion as the information regarding the nucleotide sequences of several CaHV-1 genes [10,[12][13][14].…”

Section: Glycoprotein Cmentioning

confidence: 99%

Use of Primer Design to Detect the Glycoprotein C Gene of Canine Herpes Virus by Polymerase Chain Reaction

Vargas¹,

Jara²,

Navarro³

2022

Act Sci VetSci

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The fundamental difference between a virus and microorganisms lies in the way they generate progeny and some viruses destroy the cell membrane, a phenomenon called cytolysis. The herpes virus has this cytolytic capacity and in veterinary medicine, several important herpes viruses have been described, among which is the Canine Herpes Virus type 1 (CaHV-1) and this study contributed both to the genomic characterization of a national isolate of the CaHV-1 virus -called RP5-as well as to small animal veterinary medicine with a specific, sensitive, and fast diagnostic method result. For this, detection of the CaHV-1 glycoprotein C gene was performed by Polymerase Chain Reaction using a pair of primers designed in silico. Using a temperature gradient thermocycler, the alignment temperature (55ºC) was established by the clear and unequivocal observation of a DNA fragment of approximately 200 bp. These fragments were sequenced and a percentage of nucleotide identity (NIP > 94%) was established with respect to the official GenBank data and indicates that the amplified fragment corresponds to the gC gene fragment of CaHV-1. Thus, this methodology constitutes another technique of choice for the detection of CaHV-1 in Small Animal Medicine.

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Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil

Abstract: ABSTRACT.-Cargnelutti J.F., Masuda E.K., Neuls M.G., Weiblen R. & Flores E.F. 2015. Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil. Pesquisa

Cited by 6 publications

References 10 publications

Detection of respiratory viruses in shelter dogs maintained under varying environmental conditions

Detection of respiratory viruses in shelter dogs maintained under varying environmental conditions

The first molecular detection of Canine herpesvirus-1 in reproductive specimens of adult dogs in Iran

Use of Primer Design to Detect the Glycoprotein C Gene of Canine Herpes Virus by Polymerase Chain Reaction

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