Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus)

Abstract

RESUMO.-[Produção lacrimal, pressão intraocular, microbiota, citologia e histologia conjuntival de coelhos Nova Zelândia.] O objetivo deste estudo foi estabelecer valores de referência para testes diagnósticos oftálmicos em coelhos da raça Nova Zelândia (Oryctolagus cuniculus). 22 coelhos, machos, adultos foram utilizados. Foi mensurada a produção lacrimal através do teste lacrimal de Shirmer 1 (TLS1) e da Tira endodôntica de papel absorvente (EA-PPTT) de duas marcas comerciais distintas. The purpose of this study was to establish reference values for selected ophthalmic diagnostic tests in New Zealand rabbits (Oryctolagus cuniculus). A total of 22 adult male rabbits were used. The ophthalmic tests included evaluation of tear production with Schirmer tear test 1(STT1) and Endodontic absorbent paper point tear test (EAPPTT) using two different commercial brand materials. Applanation tonometry, Culture of the conjunctival bacterial flora, , conjunctival cytology and conjunctival histology were also performed. Mean (±SD) for STT1, EAPPTTa, EAPPTTb and IOP was 7.27±2.51mm/min, 12.43±1.69mm/min, 15.24±2.07mm/min, 12.89±2.80mm Hg, respectively. Staphylococcus epidermidis, Staphylococcus sp. and Bacillus sp. were predominant. The cytological evaluation revealed the presence columnar epithelial cells, superficial squamous keratinized cells, lymphocytes, heterophils, red blood cells, mucus and bacteria. The histological analysis revealed a stratified epithelium, characterized by the presence of columnar epithelial cells with a large number of goblet cells. The reported data can be used for therapeutic or experimental purposes.INDEX TERMS: Tear production, intraocular pressure, conjunctival microbiota, New Zealand rabbits, Oryctolagus cuniculus, lagomorphs, ophthalmology, Schirmer tear test, tonometry, endodontic paper point. Tear production, intraocular pressure and conjunctival microbiota, cytology and histology of New Zealand rabbits (Oryctolagus cuniculus) histologia revelou epitélio estratificado caracterizado pela presença de células epiteliais colunares com grande núme-ro de células caliciformes. Os achados deste estudo poderão ser utilizados com fins terapêuticos ou experimentais.

Bacterial culture and antibiotic sensitivity from the ocular conjunctiva of horses

“…The grampositive bacteria displayed a predominance of 86.6% among the strains isolated, in accordance with findings for a series of domestic and wild animals (PRADO et al, 2005;SANTOS et al, 2009;SPINELLI et al, 2010;BONELLI et al, 2014;ORIÁ et al, 2014).…”

“…In different animal species, including horses (ANDREW et al, 2003;JOHNS et al, 2011), mules (TAMARZADEH & ARAGHI-SOOREH, 2014), donkeys (FOTI et al, 2013), sheep (BONELLI et al, 2014), dogs (PRADO et al, 2005) and rabbits (ORIÁ et al, 2014). Bacterial population often isolated from healthy eyes is composed of gram-positive bacteria, although strains of methicillin-resistant to Staphylococcus (MRS) and gran negative strains, have also been identified (SANTOS et al, 2009;JOHNS et al, 2011;WILLCOX, 2013;MOUNEY et al, 2013).…”

Morphological study of the upper, lower and third eyelids in the African black ostrich (Struthio camelus camelus L., 1758) (Aves: Struthioniformes) during the embryonic and postnatal period

“…The ocular immune system protects the eye from different diseases, which cause conjunctivitis e.g. bacterial, viral and toxic infections, allergy, and dry-eye syndrome in both humans and animals (Aşti et al 2000a,b;Bayraktaroglu & Aşti 2009;Siebelmann et al 2013;Oria et al 2014). Conjunctivitis is very common in domestic, ornamental and wild birds, and may be a symptom of many other medical problems, particularly respiratory infections.…”

Microbiota of the conjunctival sac of 120 healthy cats

“…42,43 The second most isolated bacterium was Micrococcus luteus, a nonpathogenic resident of the skin surface, inanimate surfaces, and soil. 44,45 In dogs, 8,17,27 rabbits, 46,47 horses, 11,12,31 sheep, 41 bats, 3 and elephants, 48 Micrococcus luteus has been commonly isolated previously. To the author's knowledge, this is the first report of Micrococcus luteus isolated from the ocular surface of cats.…”