Stereological study of the elastic fiber and smooth muscle cell system in the bovine and buffalo penis

Abstract

Pesq. Vet. Bras. 33(Supl.1):107-112, dezembro 2013 107 RESUMO.-[Estereologia do sistema elástico e das célu-las musculares lisas no pênis fibroelástico zebuino e bubalino.] O pênis dos zebuinos e bubalinos são classificados como do tipo fibroelástico, sendo grande parte de sua rigidez conferida pelas fibras colágenas e elásticas presentes na matriz extracelular. Embora alguns estudos tenham abordado estes componentes em bovinos europeus, os dados na literatura sobre zebuínos e bubalinos são escassos. Nós avaliamos a espessura da túnica albugínea, a distribuição das células musculares lisas e a densidade volumétrica (Vv) das fibras do sistema elásticona túnica albugínea, no corpo cavernoso e esponjoso do pênis de animais jovens adultos inteiros.Foram utilizados 10 fragmentos de pênis de búfalos da raça Mediterrâneo e 10 fragmentos de pênis de bovinos da raça Red Sindi. Os fragmentos de pênis foram processados de acordo com a técnica histológica de rotina para inclusão em parafina e corados pela Fucsina Resorcina de Weigert, Picro Sirius Red, Tricrômio de Gomori e anticorpo anti α actina. As fibras elásticas foram determinadas em 25 campos aleatórios de cada fragmento, utilizando o sistema teste modelo M42. A Vv das fibras do sistema elástico no pênis do búfalo e bovino foram(média e desvio padrão respectivamente) de 4,07% ±0,88 e 3,36% ±1,21 na túnica albugínea; 17,32% ±2,21 e 13,14% ±1,27 no corpo cavernoso; 26,58% ±4,31 e 31,36% ±3,67 no corpo esponjoso, respectivamente. Houve diferença na Vv das fibras elásticas entre as duas espécies, no corpo cavernoso e no corpo esponjoso. Na espessura da túnica albugínea houve diferença significativa entre o búfalo Mediterrâneo (1,4mm ±0,11) e o bovino Red Sindi (1,76mm ±0,23). Nas duas espécies, as fibras musculares lisas foram encontradas apenas associadas ao endotélio de revestimento dos seios cavernosos. Com base nestes resultados, podemos concluir que existem diferenças na proporção entre os componentes da matriz extracelular nas espécies estudadas, fato que deve ser considerado nos procedimentos clínicos e cirúrgicos veteriná-rios e continua a ser investigado. Samples of ten penises of Mediterranean buffaloes and ten penises of Red Sindhi cattle were used. The thickness of the tunica albuginea (TA), distribution of smooth muscle cells (SMC) and volume density (Vv) of elastic system fibers in TA, corpus cavernosum (CC) and corpus spongiosum (CS) were evaluated. The Vv of elastic system fibers in buffalo and bovine penis was respectively 4.07% ±0.88% and 3.36% ±1.21% in TA; 17.32% ±2.21% and 13.14% ±1.27% (CC), 26.58% ±4.31% and 31.36% ±3.67% (CS). The CC of buffalo presented higher Vv of elastic fibers than bovine, while in the CS the Vv of elastic fibers in buffaloes was smaller than in cattle. The TA thickness showed a significant difference among the species studied. The arrangement of SMC in the bovine penises and in the water buffalo suggests that this pattern is common to animals that have fibroelastic penises.

Histochemical Studies on Penis of the Boar (Sus Scrofa Domesticus)

“…The collagen fibers were arranged circularly in superficial layer while were longitudinally oriented in the deeper layer of the tunica albuginea. These observations are in partial agreement with the findings of Ziehmer et al (2013) and Ribeiro et al (2013) who cited that smooth muscle fibers and large amount of elastic connective tissue occurred in the penis of Babirusa pigs and buffalo respectively.…”

“…The tunica albugenia consisted of numerous myelinated nerve fibers in bundles and arteries. These observations were also made by Babinski et al (2004) in wild boar, Kalita et al, (2015) in Zovawk, Ashdown and Gilanpour (1974) in bull, Ziehmer et al (2013) in Babirusa and Ribeiro et al (2013) in buffalo. The extent of the septum was very short as it disappeared immediately resulted in the formation of a single cavernous body at the body and tip of the penis.…”

“…Babirusa (Ziehmer et al, 2013) and buffalo (Ribeiro et al, 2013). The trabeculae form a median septum, which is present only near the route of the penis of ruminants and boars (Trautmann and Fiebiger, 1957).…”

Histological and morphometric evaluation of the urethra and penis in male New Zealand White rabbits

“…The penis of rabbits is of musculocavernous type (Farag, Elsayed, & Shoeib, 2017), as the spaces filled with blood are relatively large and the septa separating them are delicate and muscular (Dyce, Sack, & Wensing, 2009; Hafez & Hafez, 2013; Ribeiro, Abidu‐Fig ueiredo, Costa, Pereira‐Sampaio, & Chagas, 2013). Furthermore, smooth muscles form the most abundant component of the penile erectile tissue.…”

Anatomy of the cavernous muscles of the kangaroo penis highlights marsupial–placental dichotomy

“…In mammals with a fibroelastic penis (e.g. domestic cattle and pigs), the thick fibroelastic tissues exert pressure on the engorged vascular spaces and limit change in girth, resulting in rapid lengthening of the penis and straightening of the sigmoid flexure (Maia et al 2006;Ribeiro et al 2013). By contrast, in animals with a musculocavernous penis (e.g.…”