Oxidative metabolism and muscle biochemical profile of polo horses supplemented with an ADE vitamin complex

Abstract

Pesq. Vet. Bras. 33(Supl.1):58-62, dezembro 2013 58 RESUMO.-[Metabolismo oxidativo e perfil bioquími-co muscular de cavalos de pólo suplementados com complexo vitamínico ADE.] O jogo de pólo se caracteriza por mudanças abruptas e frequentes na intensidade do exercício dos cavalos. Para satisfazer esta demanda inconstante de energia, os animais utilizam as vias aeróbia e anaeróbia em proporções e intensidade variáveis. Neste contexto deve haver equilíbrio entre a formação das espé-cies reativas de oxigênio (EROs) e a ação das substâncias antioxidantes a fim de evitar o estresse oxidativo e suas consequências. Avaliou-se o efeito da suplementação com vitaminas ADE no metabolismo oxidativo destes animais. Para tanto, 18 equinos mestiços foram distribuídos aleatoriamente em dois grupos: tratado e controle (GT) e controle (GC).Os animais do GT receberam complexo vitamínico ADE (1 mL/50 kg de peso vivo) pela via intramuscular Horses used for the game of polo experience abrupt and frequent changes in exercise intensity. To meet this variable energy demand, the horses use both aerobic and anaerobic pathways in varying proportions and intensities. In this context, there must be a balance between the formation of reactive oxygen species (ROS) and the action of antioxidants to prevent oxidative stress and its consequences. The effect of supplementation with an ADE vitamin complex on oxidative metabolism was evaluated in 18 crossbred horses randomly divided between a treated group (TG) and a control group (CG). The TG animals received the ADE vitamin complex (1mL/50 kg of body weight) by deep intramuscular injection at 30 and 15 days before the game. The CG horses received 10ml of saline by the same administration route and schedule. During the polo match, the animals played for a total of 7.5 min. Blood samples were collected on the same days as the treatments were administered, and immediately before and at 15, 90 and 180 minutes after the game. The concentrations of creatine phosphokinase (CK), lactate dehydrogenase (LDH), lactate, glucose, aspartate aminotransferase (AST), glutathione (GSH), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in the blood samples. After the game, the TG demonstrated higher levels of AST, lactate and glucose than the CG, suggesting more efficient energy use by the treated animals. The higher GSH and lower lactate levels in the TG before the game suggest the presence of a greater antioxidant supply in the treated animals. The maintenance of the MDA levels indicates that neither of the groups exhibited oxidative stress.

Health of Polo Horses

“…It was found that supplementation with the ADE vitamin complex in a dose of 1 mL/50 kg of live weight (270,000 IU of vitamin A, 80,000 IU of vitamin D, and 80 IU of vitamin E/mL) via intramuscular injection can make muscle metabolism more efficient during exercise, suggesting a possible therapeutic approach [ 59 ]. After the game, the polo horses in the treated group had higher AST, lactate, and glucose levels than the horses in the control group.…”

“…This indicates that the treated animals used their energy more efficiently. Before the game, the animals in the treated group also had higher GSH levels and lower lactate levels, indicating a better anti-oxidant supply [ 59 ].…”