Aspergillus fumigatus from normal and condemned carcasses with airsacculitis in commercial poultry

Abstract

Pesq. Vet. Bras. 33(9):1071-1075, setembro 2013 1071 RESUMO.-[Pesquisa de Aspergillus fumigatus em carcaças de frango de corte normais e condenadas por aerossaculite.] Nos abatedouros, a inspeção das carcaças é fundamental para a detecção e monitoramento da prevalência de certas doenças. Os objetivos do trabalho foram avaliar a ocorrência de aspergilose causada por Aspergillus fumigatus em aves comerciais através do diagnóstico micológico e histopatológico e verificar a possibilidade de associação causal entre os critérios de diagnóstico de aspergilose e condenação por aerossaculite em frangos de corte através de um estudo de caso-controle. O estudo foi realizado com 380 amostras. Foram coletados pulmões de frangos condenados (95) e não condenados (285) por aerossaculite, diretamente na linha de abate de um frigorífico. Quarenta e seis (12%) amostras de pulmão foram positivas na cultura micológica. Do total de amostras, 177 (46,6%) apresentaram alterações histopatológicas, sendo os mais frequentes pneumonia fibrinoheterofílica necróti-ca, pneumonia heterofílica e hiperplasia linfóide. Do total de 380 pulmões analisados, 65,2% (30) Carcass inspection is important for the detection of certain diseases and for monitoring their prevalence in slaughterhouses. The objective of this study was to assess the occurrence of aspergillosis caused by Aspergillus fumigatus in commercial poultry, through mycological and histopathological diagnosis, and to verify the causal association between the aspergillosis diagnosis criteria and condemnation due to airsacculitis in broilers through a case-control study. The study was carried out with 380 samples. Lungs were collected from broilers that were condemned (95) or not condemned (285) due to airsacculitis directly from the slaughter line. Forty-six (12%) lung samples were positive for A. fumigatus in mycological culture. Among all samples, 177 (46.6%) presented histopathological alterations, with necrotic, fibrinous, heterophilic pneumonia; heterophilic pneumonia and lymphoid hyperplasia being the most frequent. Out of the 380 lungs analyzed, 65.2% (30) showed histopathological alterations and isolation of fungi. The statistical analysis (McNemar's chi-square test) indicated a significant association between the presence of histopathological lesions and the isolation of A. fumigatus. Mycological cultivation and histopathological diagnosis increase the probability of detecting pulmonary alterations in birds condemned by the Final Inspection System, which suggests that such diagnostic criteria can improve the assessment and condemnation of birds affected by airsacculitis.

Histological Investigation of Pneumonia in Domestic Ducks (Anas platyrhynchos domesticus) in Basrah Province, Iraq

“…The current histochemical investigations showed formation of thick fibrous connective tissue surrounded the bronchus revealed to peribronchial fibrosis as well to interstitial infiltration of mucoid like substances in the pulmonary parenchyma, these findings may resulted from the inflammatory defence mechanism against to invaders in order to ameliorate it harmful effects on the pulmonary tissues, this idea may agree with the investigations of Spanamberg et al [13] on the pathological features of pneumonia in poultry which reported a histopathological changes mostly necrotic, fibrinous, heterophilic pneumonia and pulmonary lymphoid tissue hyperplasia associated with the complicated cases of poultry pneumonia.…”

Antifungal susceptibility profile of Aspergillus fumigatus isolates from avian lungs

“…Azole resistance might be developed through the use of azole compounds in the environment and specifically in the case of birds; conditions that favor the development of fungi in confinement buildings expose commercial poultry to a higher risk of inhaling conidia of A. fumigatus during the farming period. Under these conditions, birds could inhale resistant conidia and subsequently develop azole-resistant disease or have fungal colonization in respiratory tract (Arné et al 2011, Spanamberg et al 2013. The use of fungistatic agents (sprayed, fogged, or nebulized to treat surfaces or indoor) like thiabendazole, nystatin, or copper sulfate contributes to decreased fungal contamination of beddings.…”