Características clínicas e anatomo-histopatologicas da infecção experimental mista por Orthoreovirus aviario e Mycoplasma synoviae em frangos de corte

Abstract

Pesq. Vet. Bras. 32(8):687-691, agosto 2012 687 RESUMO.-A artrite infecciosa em frangos de corte representa um problema sanitário e econômico de grande impacto, provocando perdas de produtividade e nos processos de produção e industrialização. Os principais agentes etiológicos associados aos casos de artrites e tenossinovites infecciosas em aves são Mycoplasma synoviae (MS) e Orthoreovirus aviario (ARV). Esse trabalho propôs investigar as alterações anatomohistopatológicas causadas pela infecção experimental concomitante por Mycoplasma synoviae e Orthoreovirus aviario em frangos de corte e conϐirmar a presença dos agentes através das técnicas de PCR e imunoϐluorescência indireta (RIFI Infectious arthritis in broiler represents an economic and health problem resulting in severe losses due to retarded growth and down grading at slaughterhouse. The most common agents associated with cases of infectious arthritis in poultry are Mycoplasma synoviae and Avian orthoreovirus. This study proposed to investigate the histopathological changes caused by mixed infection with Mycoplasma synoviae and Avian orthoreovirus in broilers and conϐirm the presence of the agents through PCR and immunoϐluorescence assay (IFA). We used 16 broiler chickens, housed in bed, with supply of food and water ad libitum. Ten-day-old broilers were infected with Mycoplasma synoviae and Avian orthoreovirus. Clinically, they showed lethargy and swelling of the hock joint. After 30 days, we proceeded to their euthanasia and necropsy. Histological lesions were observed due to the mixed infection with Mycoplasma synoviae and Avian orthoreovirus in different tissues. The histopathology of the joints was characterized by inϐiltration of heterophil leucocytes in the synovial membrane and the digital ϐlexor tendon. The inϐlammatory process was also found in trachea, lungs, air sac, liver, spleen, pericardium and proventriculus. The use of IFA was necessary to verify the presence of both agents in the hock joints, identifying the antigens of Mycoplasma synoviae and Avian orthoreovirus. The presence of M. synoviae was detected by PCR in trachea, lung, air sacs, synovial membrane and synovial ϐluid. Avian orthoreovirus was detected with PCR in liver, spleen, synovial membrane and digital ϐlexor tendon. In conclusion, this investigation suggests that a synergistic relationship exists between Mycoplasma synoviae and Avian orthoreovirus.

Molecular detection of Mycoplasma synoviae and avian reovirus infection in arthritis and tenosynovitis lesions of broiler and breeder chickens in Santa Catarina State, Brazil

“…Histopathological investigations confirmed the presence of infectious arthritis (Figure 3). Additionally, observations, particularly, the diffuse lymphohistiocytic infiltrate with heterophil accumulation in the synovial capsule and digital flexor tendon of samples, that are PCR-positive for M. synoviae and ARV, support the diagnosis of infectious arthritis caused by both avian pathogens (Bradbury & Garuti 1978; Ni & Kemp 1995; Reck et al 2012).…”

“…Co-infection with M. synoviae and ARV is often observed in turkey and chicken flocks, and may be associated with severe cases of arthritis or tenosynovitis and systemic syndromes, including chronic respiratory disease and decreased growth (Landman & Feberwee 2012; Reck et al 2012). We observed mixed infection from arthritic lesions in both broilers (7.7%) and breeders (12.2%) (Figure 1).…”

“…Cell-mediated immune responses play crucial roles in the control of both M. synoviae and ARV infections and are involved in immunopathologic responses associated with the formation of arthritic lesions (Reck et al 2012; Senties-Cue, Shivaprasad & Chin 2005). Mixed infection by M. synoviae and ARV in chickens results in exacerbation of clinicopathological effects (Moreira et al 2017; Reck et al 2012), suggesting a possible synergistic interaction of these two pathogens in immunosuppression (Ni & Kemp 1995; Senties-Cue et al 2005). In the present study, cases of ARV infection occurred mainly as part of mixed infection with M. synoviae in both broilers and breeders.…”

“…Avian reoviruses (ARVs), a type of ribonucleic acid (RNA) virus, are associated with severe arthritis or tenosynovitis, chronic respiratory diseases, leg weakness, immunosuppression and malabsorption syndrome (Landman & Feberwee 2012; Zhong et al 2016). Mixed infection caused by both M. synoviae and ARV in chickens results in exacerbation of clinicopathological effects (Dobson & Glisson 1992; Moreira et al 2017; Reck et al 2012).…”

Artrite Infecciosa Em Frangos De Corte

“…Um exemplo de patologia que causa as perdas acima citadas é a artrite infecciosa que na avicultura industrial é um problema sanitário e econômico que provoca grandes perdas nos processo de produção e industrialização, sendo uma das causas de condenação patológica mais frequente em abatedouros frigoríficos (RECK et al, 2012). Na mesma temática, SILVA & VIEIRA (2010) descreveram que os prejuízos econômicos ultrapassam milhões de reais e são incompatíveis com a competitividade da avicultura.…”