Serum protein concentrations, including acute phase proteins, in calves experimentally infected with Salmonella Dublin

Abstract

The aim of this study was to evaluate serum protein concentrations in calves experimentally inoculated with Salmonella Dublin. Twelve healthy 10 to 15-day-old Holstein calves were randomly allotted into two groups, control and infected with 10(8) CFU of Salmonella Dublin orally. The calves were subjected to physical evaluation and blood samples were collected shortly before administration of the bacteria and also 24, 48, 72, 96, 120 and 168 hours post-infection. The concentration of serum proteins was determined through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Thirty serum proteins ranging from molecular weight of 24,000 Da to molecular weight of 236,000 Da were detected. Serum concentrations of ceruloplasmin (125,000 Da), haptoglobin (45,000 Da), acid glycoprotein (40,000 Da) and a 34,000 Da protein were significantly increased in the experimentally infected calves, when compared with their concentrations in the control animals. Therefore, this study showed that S. Dublin infection could lead to the increase of certain serum proteins in calves.

Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea

“…In other studies evaluating blood serum proteinogram through SDS-PAGE, of calves affected by different infectious causes of diarrhea, Ávila (2009) and Silva et al (2011) reported an increase in ceruloplasmin concentrations in Holstein calves experimentally infected with Salmonella Typhimurium and Salmonella Dublin, respectively. Hajimohammadi et al (2013) also found a significant increase in ceruloplasmin levels in calves that presented diarrhea, when compared to healthy animals.…”

“…Specifically referring to rotavirus infection, Westerman et al (2005) working with non-human primates reported that an increment in concentration of serum IgG elevated immunoglobulin titers reaching the intestinal lumen and avoided or reduced rotavirus shedding.…”

Blood serum acute phase proteins and iron dynamics during acute phase response of Salmonella enterica serotype Dublin experimentally infected buffalo calves

“…Haptoglobin is a major APP in ruminants (Eckersall et al, 2001;Eckersall and Bell, 2010;Ceciliani et al, 2012) and is also considered a good indicator of inflammation in buffaloes (Khan et al, 1997). In bovine and buffaloes, increases in serum haptoglobin have been positively correlated with the severity of clinical signs of salmonellosis (Deignan et al, 2000;Silva et al, 2011;Clemente et al, 2016). Therefore, considering our findings and other published studies, all indicates that this protein can be a useful non-specific biomarker of Salmonella spp.…”

“…In bovines, studies showed the serum protein profile (Silva et al, 2011) and iron concentrations (Silva et al, 2010) of infected Salmonella Dublin newborn calves and observed hypoferremia and increase of serum ceruloplasmin, haptoglobin, α1-acid glycoprotein and fibrinogen during the infection. In contrast, in buffaloes, there are few studies on APP dynamics (El-Deeb and Iacob, 2012;Tajik et al, 2012;Kumar et al, 2014), especially with simultaneous assessment of ferremic response (Horadagoda et al, 2002;Clemente et al, 2016).…”

Erythrogram, leukogram, and acute phase protein reference intervals for healthy newborn Murrah buffalo calves (Bubalus bubalis) within the first month of life

“…For example, modifications of blood serum components in diarrheic newborn bovines infected with Salmonella dublin (Silva et al 2010(Silva et al , 2011 and newborn buffaloes infected with Salmonella thyphimurium (Clemente et al 2016) have been studied. Important alterations have been linked to inflammation and infection, such as increases in acute phase proteins (APP) fibrinogen, ceruloplasmin and haptoglobin, and leukocytosis.…”