Detection and dynamics of porcine circovirus 2 shedding in semen using conventional and real-time PCR

Gerber, Priscilla F.; Pinto, Flávia Ferreira; Heinemann, Marcos Bryan; Lobato, Zélia Inês Portela

doi:10.1590/s0100-736x2010001100004

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…O significado epidemiológico de disseminação do organismo a partir do trato urogenital e a avaliação da eficácia de antibióticos na supressão do organismo de sêmen processado para inseminação artificial são também dignos de investigação (HUMPHREY et al, 1982). Os dados da literatura (DIAS, 2005;SAUNDERS et al, 2007) mostram que essa bactéria é um patógeno sexualmente transmissível e reforçam a necessidade de mais estudos sobre a possível ocorrência de transmissão outras espécies, a PCR foi usada em pesquisas recentes na detecção de vírus em javali (GERBER et al, 2010), Toxoplasma gondii no carneiro (MORAES et al, 2010) e bactérias em ovinos (SAUNDERS et al, 2007).…”

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Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR)

Dias

Nunes

Cavalcante

et al. 2014

Rev. Acad. Ciênc. Anim.

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Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR) [I] Detection of Histophilus somni (Haemophilus somnus) in bovine semen using polymerase chain reaction (PCR) [a] ResumoO presente estudo avaliou o uso da PCR para a detecção do Histophilus somni no sêmen bovino. Amostras de sêmen foram contaminadas experimentalmente com H. somni diluída em escalas de 10 7 a 10 1 bactérias/ mL, submetidas à extração de DNA pelo método de fenol/clorofórmio e amplificadas pela PCR. Os produtos da amplificação do DNA foram analisados por eletroforese em gel de acrilamida 8%. Por meio de oligonucleotídeos iniciadores obteve-se a amplificação de um fragmento de 400 pares de bases a partir do DNA da bactéria. Conseguiu-se amplificação positiva até na diluição de 10 1 bactérias/mL. A PCR mostrou-se eficiente na detecção de H. somni. O resultado disponibiliza conhecimento relevante para o diagnóstico de H. somni,

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“…Gerber et al (2010) utilizaram a PCR para a detecção de vírus, Mukhufhi et al (2003) para detecção de protozoários e Saunders et al (2007) utilizaram a técnica de reação em cadeia da polimerase múltipla (mPCR) para obter a padronização da detecção simultânea de Brucella ovis, Actinobacillus seminis e H. somni em sêmen fresco de ovinos.…”

Section: Introductionunclassified

Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR)

Dias

Nunes

Cavalcante

et al. 2014

Rev. Acad. Ciênc. Anim.

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“…The present study evaluated the occurrence of natural infection of PPV4 virus in boar semen and provided evidence of intermittent elimination as happens with other porcine DNA-viruses, such as porcine circovirus and Torque Teno Sus Virus Species k2 (TTSuVk2) (MADSON;OPRIESSNIG, 2011;ROSE et al, 2012;GERBER et al, 2010).…”

Section: A1mentioning

confidence: 99%

Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first report

Castro

Bersano

Feijó

et al. 2018

SCA

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Porcine parvovirus 4 (PPV4) has been reported in several countries and the high rate of concurrent PCV2 infection with PPV4 may trigger the “porcine circovirus disease” (PCVD). This has awakened the interest in how PPV4 virus behaves. The aim of this study was to show that, like other swine viruses, the elimination of PPV4 in semen is intermittent. The study was conducted in the Unidade de Pesquisa e Desenvolvimento de Itapeva da Agência Paulista de Tecnologia dos Agronegócios Regional (Apta Regional). Over a period of six months, four boars were monitored and the semen was collected every 10 days, totalizing 74 semen samples. Through PCR testing, PPV4 was detected in 32/74 (43.2%) semen samples. Three sequences of 284 nt, showed identity varying from 98.2% to 100%, confirming the detection of the virus in all boars. Semen volume and sperm motility did not indicate significant difference between PPV4 positive or negative semen samples (p > 0.05). There was no significant difference between the monthly distributions of positive and negative samples. This is the first study showing intermittent shedding of PPV4 in naturally infected boars. Absence of typical clinical signs and/or influence in semen quantity was also observed.

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“…Mature boars infected with PCV2 (naturally or experimentally) are often free of clinical signs but they can shed the virus in semen for a long period (Gerber et al 2010). The PCV2 viral antigen has been detected in the reproductive organs (testes) and tissues (germinal epithelial cells, epididymis) of naturally and experimentally infected boars.…”

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confidence: 99%

The effect of porcine circovirus type 2 (PCV2) vaccination of male piglets on sperm quality at the age of puberty

et al. 2019

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The objective of the study was to investigate the effect of porcine circovirus type 2 (PCV2) vaccination in piglets on sperm quality of young boars. A total of 136 sows were divided in four groups of 34 animals each (17 vaccinated with Circovac®, Merial and 17 unvaccinated in each group). A total of 1 200 piglets were selected, half of which were vaccinated against PCV2 on the 21st day (Porcilis® PCV, MSD) and the other half was left unvaccinated. Four groups of 300 pigs each were formed as follows: PS group (vaccinated sows + piglets), S group (vaccinated sows + unvaccinated piglets), P group (unvaccinated sows + vaccinated piglets), C group (unvaccinated sows + piglets). Furthermore, 80 boars (20 piglets per group) were selected and slaughtered at the age of 5.5 months and weight of 95 ± 5.5 kg and their epididymal sperm was collected and evaluated for motility and kinetics, concentration and morphology. Additionally, 10 pigs from each group were used for blood sampling and serological testing for PCV2 IgM and IgG antibodies at the age of 21, 70, 110 and 150 days. The IgG and IgM patterns suggested that the piglets were coming into contact with PCV2 early in life. The S group demonstrated significantly lower curvilinear velocity (VCL, μm/s), amplitude of lateral head displacement (ALH, μm) and significantly higher head abnormalities (%) compared to other groups (P < 0.05). In conclusion, vaccinated young boars showed some improved epididymal sperm kinetic indices and head morphology.

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Detection and dynamics of porcine circovirus 2 shedding in semen using conventional and real-time PCR

Cited by 3 publications

References 10 publications

Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR)

Detecção de Histophilus somni (Haemophilus somnus) no sêmen bovino mediante reação em cadeia pela polimerase (PCR)

Intermittent elimination of Porcine Parvovirus 4 (PPV4) in naturally infected swine semen: first report

The effect of porcine circovirus type 2 (PCV2) vaccination of male piglets on sperm quality at the age of puberty

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