Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5

Holz, Carine Lidiane; Cibulski, Samuel Paulo; Teixeira, Thaís Fumaco; Batista, Helena Beatriz de Carvalho Ruthner; Dezen, Diogenes; Campos, Fabrício Souza; Varela, Ana Paula Muterle; Roehe, Paulo Michel

doi:10.1590/s0100-736x2010000700001

Cited by 4 publications

(2 citation statements)

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“…In addition, particularly in tropical countries, the number of buffalo breeding farms is increasing, in most instances close to, or in conjunction with, bovine cattle farming. The extensive antibody cross-reactivity induced by infections with any of the herpesviruses examined here has already been demonstrated, both here and in previous studies [ 9 , 33 , 35 ]. Moreover, cross-reactive antibodies may interfere with the trade and exports of live animals, where some countries require the animals to be fully seronegative to BoAHV-1.…”

Section: Discussionsupporting

confidence: 81%

An ELISA to Detect Antibodies to Bovine Alphaherpesviruses 1 and 5 and Bubaline Alphaherpesvirus 1 in Cattle Sera

Scheffer

Petzhold

Varela

et al. 2023

Veterinary Sciences

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Bovine alphaherpesvirus 1 (subtypes 1.1, 1.2a, and 1.2b), type 5 (subtypes 5a, 5b, and 5c), and bubaline herpesvirus 1 (BuHV-1) induce highly, though not fully cross-reactive serological responses. Most types and subtypes of these viruses circulate particularly in countries of the southern hemisphere, notably Brazil and Argentina. Therefore, the detection of infected animals is important in defining prevention and control strategies, particularly when flocks are destined for international trade. Identification of infected herds is most often achieved by assays that detect antibodies, such as enzyme immunoassays (ELISAs). However, to date, no ELISA has been evaluated in its capacity to detect antibodies to these alphaherpesviruses. Here, an ELISA was developed to detect antibodies to all currently recognized BoAHV-1, BoAHV-5, and BuAHV-1 types/subtypes, and its sensitivity and specificity were determined. Six hundred bovine sera were screened in serum neutralization tests (SN) against the seven viruses. ELISAs prepared with each of the viruses were compared to SN. Subsequently, a combined assay with multiple antigens LISA was prepared by mixing five viral antigens, chosen for their highest sensitivity in the preparative assays. In comparison to SN, the mAgELISA sensitivity was 96.5% with 96.1% specificity (κ = 0.93; PPV = 95.0%; NPV = 97.3%). The findings reveal that the mAgELISA developed here is highly suitable for the detection of antibodies, comparable in sensitivity and specificity to that of SN when performed with all known types and subtypes of bovine and bubaline alphaherpesviruses.

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Section: Discussionsupporting

confidence: 81%

An ELISA to Detect Antibodies to Bovine Alphaherpesviruses 1 and 5 and Bubaline Alphaherpesvirus 1 in Cattle Sera

Scheffer

Petzhold

Varela

et al. 2023

Veterinary Sciences

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“…A infecção também pode ser diagnosticada através de testes sorológicos do BoHV-1 por ELISA e soro-neutralização (SN) (BASHIR et al, 2011;FLORES, 2007;PARREÑO et al, 2010;ROCHA et aL., 2001). O teste de soroneutralização (SN) é o método padrão para a mensuração de anticorpos neutralizantes para herpesvírus bovino (HOLZ et al, 2010).…”

Section: Introductionunclassified

Registro de Herpesvírus Bovino (BoHV-1 e BoHV-5) em Rebanho Leiteiro de Propriedades Agro Familiares da Cidade de Alegrete – RS

Duarte

Santana

2018

Ens Cien C Biol Agra Saúde

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As doenças reprodutivas se constituem um dos principais motivos de prejuízo na bovinocultura, representando uma parcela significativa da diminuição dos índices de prenhes e animais nascidos. Entre estas doenças, a infecção por Herpesvírus Bovino (BoHV) tem grande destaque, principalmente, por sua grande disseminação no rebanho brasileiro. O BoHV-1 é o agente etiológico relacionado com a rinotraqueíte infecciosa bovina (IBR), aborto e reabsorção embrionária, além de infecções em nível reprodutivos e neonatais. Já o BoHV-5 é o agente responsável pela encefalite herpética bovina. Entre os diversos métodos de diagnóstico, a soroneutralização é utilizada rotineiramente para evidenciar a presença de anticorpos neutralizantes para o vírus. O presente trabalho teve por finalidade diagnosticar a prevalência sorológica por Soroneutralização (SN), referente ao herpesvírus bovino tipo 1 e tipo 5 no rebanho leiteiro, não vacinado, de propriedades agro familiares de assentados da reforma agrária localizados na cidade de Alegrete - RS. Nas avaliações realizadas para o presente estudo foram registradas proporções de 40,90% e 39,39% de animais soropositivos para os agentes BoHV-1 e BoHV-5, respectivamente. Os resultados obtidos sugerem que é necessário maior empenho no controle da enfermidade, uma vez que possui fácil disseminação. Além disso, os dados evidenciam o cenário da bovinocultura brasileira, em que há presença significativa de agentes potenciais, que resultam em diminuição das taxas reprodutivas, somada a uma cultura de não prevenção. Palavras-chave: Virologia. Herpesvírus Bovino 1. Herpesvírus Bovino 5. AbstractThe reproductive diseases are one of the main reasons for the losses in bovine culture, being a significative part of the reduction in the numbers of pregnant and newborn animals. Among those diseases, the Bovine Herpesvirus infection (BoHV) is on the spotlight, mainly because of the great dissemination in the Brazilian herd. BoHV-1 is the etiological agent related to the infectious bovine rhinotracheitis (IBR), abortion and embryony reabsorption, besides infections at reproductive and neonatal levels. Whereas, BoHV-5 is the agent responsible for the bovine herpetic encephalitis. Among the various methods of diagnosis, serum neutralization is routinely used to evidence the presence of neutralizing antibodies to the virus. The present work had the goal to diagnose the serological prevalence by Serum Neutralization (SN), referring to bovine herpesvirus type 1 and type 5 in the non-vaccinated dairy herd, from agrarian reform settlers of the agrarian reform located in the city of Alegrete – RS. In the evaluations carried out for the present study, ratios of 40.90% and 39.39% of seropositive animals were recorded for BoHV-1 and BoHV-5 agents, respectively. The results obtained suggest that a greater effort is required in the disease control disease, since it has an easy dissemination. In addition, the data show that the scenario of Brazilian cattle breeding, in which there is a significant presence of potential agents that result in a decrease in reproductive rates, together with a culture of non-preveiton.Keyword: virology. Herpesvirus 1; Bovine; Herpesvirus 5 Bovine.

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Neutralizing antibodies to bovine and bubaline alphaherpesviruses in water buffaloes (Bubalus bubalis)

et al. 2023

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Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5

Cited by 4 publications

References 24 publications

An ELISA to Detect Antibodies to Bovine Alphaherpesviruses 1 and 5 and Bubaline Alphaherpesvirus 1 in Cattle Sera

An ELISA to Detect Antibodies to Bovine Alphaherpesviruses 1 and 5 and Bubaline Alphaherpesvirus 1 in Cattle Sera

Registro de Herpesvírus Bovino (BoHV-1 e BoHV-5) em Rebanho Leiteiro de Propriedades Agro Familiares da Cidade de Alegrete – RS

Neutralizing antibodies to bovine and bubaline alphaherpesviruses in water buffaloes (Bubalus bubalis)

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