Development of IgY antibodies in chickens and IgG in rabbits immunized against proteins of Pythium insidiosum isolated from horses in the state of Rio de Janeiro

Abstract

Pesq. Vet. Bras. 30(1):87-93, janeiro 2010 RESUMO.-[Desenvolvimento de Anticorpos IgY em galinhas e IgG em coelhos imunizados contra proteí-nas de Pythium insidiosum isolado de equinos no Estado do Rio de Janeiro, Brasil.] Pitiose é causada por Pythium insidiosum e a doença foi descrita em equinos no Norte e Noroeste do Estado do Rio de Janeiro, Brasil. A doença foi descrita em bovinos, ovelhas, humanos e cavalos em diferentes estados e regiões do país. Este trabalho descreve o desenvolvimento de anticorpos policlonais, IgY e IgG, em galinha e coelho, respectivamente, contra proteínas extraídas de kunkers e hifas de P. insidiosum de cavalos doentes. As proteínas foram reconhecidas por anticorpos de galinha, coelho e cavalos contra as bandas majoritárias de 27 e 34 KDa em imunodifusão e Western blot tituladas por ELISA. Os anticorpos IgY desenvolvidos pela primeira vez contra cepas brasileiras de P. insidiosum podem representar um valioso instrumento na detecção de antígenos de patógenos e contribuem para novos estudos baseados na imunoterapia e no entendimento sobre esta doença em áreas endêmicas no Rio de Janeiro e em todo o país. INTRODUCTIONPythiosis is a granulomatous disease that affects animals and humans, caused by Pythium insidiosum, Oomycetes belonging to family Pythiaceae, with greater incidence in tropical and subtropical regions and the only described species of Pythium capable of causing disease in mammals (De Cock et al. 1987). Development of IgY antibodies in chickens and

Avian IgY antibodies: characteristics and applications in immunodiagnostic

“…of Schistosoma japonicum (CAI et al, 2012), development of IgY antibodies against proteins of Pythium insidiosum (RANGEL et al, 2010), use in antigen capture-ELISA (JULIARENA et al 2007;VEERASAMI et al, 2008), among others.…”

Growth inhibition of Staphylococcus aureus and escherichia coli strains by neutralizing IgY antibodies from ostrich egg yolk

“…coli (EPEC), enteroinvasive E. coli (EIEC), enterohemorragic E. coli (EHEC), enteroagregative E. coli (EAEC) and extraintestinal patogenic E. coli (EXPEC) are among the causes of diarrhea in human and animal leading to economic loss for farmers, including birds and mammals (18). Antibodies developed against several microorganisms in chickens, also known as IgY, has been proved to be an alternative for immunological diagnostic tool, as well as suggested as immunotherapic purposes (4, 12, 13, 15, 17, 21, 31). The aim of this work was to demonstrate for the first time the inhibitory activity of polyclonal antibodies developed in ostriches against recombinant proteins recRAP and recSEC from S. aureus and recBFPA and recEspB from E.coli , towards clinical and standard strains of S. aureus and E. coli in vitro.…”

The potential application of avian egg antibodies with emphasis on immunotherapeutic and immunodiagnostic purpose

“…Specific chicken antibodies have been successfully raised against a wide variety of antigens including proteins, peptides, lipid hormones and carbohydrate components from viruses, bacteria, fungi, plants and animals (Schade et al, 1994). Several studies have also shown promising results in the development of techniques for immunodiagnostic using IgY, such as immunoassays tests to detect circulating antigen of Schistosoma japonicum (Cai et al, 2012), development of IgY antibodies against proteins of Pythium insidiosum (Rangel, 2010), use in antigen capture-ELISA (Veerasami et al, 2008).…”