Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E

Brum, Mário Celso Sperotto; Caron, Luizinho; Chowdhury, Shafiqul I.; Weiblen, Rudi; Flores, Eduardo Furtado

doi:10.1590/s0100-736x2010000100009

Cited by 8 publications

(14 citation statements)

References 29 publications

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“…VN titers in these animals at day 30pi ranged from 16 to 1024 (GMT 5.8). As the experiment was discontinued, we were unable to retest the negative animals at a larger interval to search for late gE seroconversion, as described by Brum et al (2010b). Thus, the developed ELISA gE r was able to specifically detect gE antibodies and to differentiate serologically animals vaccinated with a gE--negative strain from those infected with gE-positive BoHV-1.…”

Section: Resultsmentioning

confidence: 99%

“…Chowdhury, unpublished, E.F. Flores, unpublished). Furthermore, gE antibodies present in sera of cattle experimentally challenged with BoHV-5 (Anziliero et al 2011) or immunized with an inactivated BoHV-5 vaccine (Brum et al 2010b) cross-reacted with BoHV-1 antigens in a commercial ELISA kit for BoHV-1 gE antibodies with sensitivity and specificity undistinguishable from that of BoHV-1 antibodies (Brum et al 2010b, Anziliero et al 2011. Hence, the recognition of the recombinant gE fragment by BoHV-1 and BoHV-5 antisera would enable its use in ELISA tests -as a companion test for BoHV-1 and BoHV-5 differential gE-deleted vaccines -in cattle populations where both viruses co-circulate.…”

Section: Discussionmentioning

confidence: 99%

“…The non-essential envelope glycoprotein E (gE) has been largely used as the antigenic marker of BoHV-1 differential vaccines (Kaashoek et al 1995, Van Oirschot 1999, Van Drunen Littel-van den Hurk 2006. In addition to provide an antigenic marker, gE deletion from BoHV-1 and BoHV-5 genomes contributes for virus attenuation (Kaashoek et al 1995, 1998, Chowdhury et al 2000, Brum et al 2010b.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, our group recently described the construction of a BoHV-5 strain lacking gE and thymidine kinase (tk) genes (Brum et al 2010b). In order to provide a serological companion test for these vaccine strains, we herein describe the expression of a truncated form of BoHV-1 gE and its evaluation as a coating antigen in an indirect ELISA for gE antibodies.…”

Section: Introductionmentioning

confidence: 99%

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Prokaryotic expression of a truncated form of bovine herpesvirus 1 glycoprotein E (gE) and its use in an ELISA for gE antibodies

et al. 2013

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Pesq. Vet. Bras. 33(1): 41-46, janeiro 2013 41 RESUMO.-[Expressão procariota de uma forma truncada da glicoproteína E (gE) do herpesvírus bovino tipo 1 e uso em ELISA para anticorpos contra a gE.] Este trabalho relata a expressão de uma forma truncada da glicoproteína E (gE) do herpesvírus bovino tipo 1 (BoHV-1) para uso em imunodiagnóstico. Um fragmento de 651 pares de bases (pb) correspondente ao terço amino-terminal (217 aminoácidos) da gE do BoHV-1 -que compartilha uma alta identidade com a gE do BoHV-5 -foi clonada como proteína de fusão com cauda 6x de histidina em um vetor de expres- This article describes the expression of a truncated form of bovine herpesvirus 1 (BoHV-1) glycoprotein E (gE) for use as immunodiagnostic reagent. A 651 nucleotide fragment corresponding to the amino-terminal third (217 amino acids) of BoHV-1 gE -that shares a high identity with the homologous BoHV-5 counterpart -was cloned as a 6×His-tag fusion protein in an Escherichia coli expression vector. A soluble protein of approximately 25 kDa purified from lysates of transformed E. coli was recognized in Western blot (WB) by anti-6xHis-tag and anti-BoHV-1 gE monoclonal antibodies. In addition, the recombinant protein was specifically recognized in WB by antibodies present in the sera of cattle seropositive to BoHV-1 and BoHV-5. An indirect ELISA using the expressed protein as coating antigen performed comparably to a commercial anti-gE ELISA and was able to differentiate serologically calves vaccinated with a gE-deleted BoHV-5 strain from calves infected with BoHV-1. Thus, the truncated gE may be useful for serological tests designed to differentiate BoHV-1/BoHV-5 infected animals from those vaccinated with gE-negative marker vaccines. bovine herpesvirus, vaccine, DIVA, recombinant protein. são em Escherichia coli. Uma proteína solúvel de aproximadamente 25 kDa purificada de lisados de E.coli foi reconhecida em Western blot (WB) por anticorpos monoclonais anti-6xHis-tag e anti-gE. Além disso, a proteína recombinante purificada foi reconhecida em WB por anticorpos presentes no soro de animais soropositivos ao BoHV-1 e BoHV-5. Um ELISA indireto utilizando a proteína recombinante como antígeno apresentou performance comparável a um ELISA gE comercial e foi capaz e diferenciar sorologicamente animais vacinados com uma cepa gE-negativa de BoHV-5 de animais infectados com o BoHV-1. Portanto, a gE truncada pode ser útil em testes sorológicos diferenciais para uso conjunto com vacinas com marcador antigênico gE para o BoHV-1 e BoHV-5.TERMOS DE INDEXAÇÃO: BoHV-5, herpesvírus bovino, vacina diferencial, proteína recombinante.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prokaryotic expression of a truncated form of bovine herpesvirus 1 glycoprotein E (gE) and its use in an ELISA for gE antibodies

et al. 2013

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“…Nevertheless, considering that the vaccine strain is not always detected (29,30), all animals showed signs and typical symptoms of respiratory deficiencies, and some animals had not been vaccinated at the time of sampling, so it is possible that those cases were experiencing the disease. On the other hand, three animals tested negative for all pathogens.…”

Section: Discussionmentioning

confidence: 99%

Detection and economic impact related to bovine respiratory disease, shrink, and traveling distance in feedlot cattle in Northwest Mexico

Rodríguez-Castillo¹,

López-Valencia²,

Monge-Navarro³

et al. 2017

Turk J Vet Anim Sci

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Bovine respiratory disease (BRD) is the main reason for economic loss in feedlots. A cross-sectional study was conducted in order to detect the pathogens involved in BRD. A total of 88 animals with respiratory signs were sampled for diagnosis using PCR. The detected pathogens were bovine respiratory syncytial virus (80.6%), Mannheimia haemolytica (79.5%), Pasteurella multocida (68.1%), parainfluenza 3 virus (23.8%), bovine herpes virus-1 (20.4%), and bovine viral diarrhea virus (11.3%). The average number of treatments applied per animal was 1.3 with an estimated treatment cost of 16 USD per treatment/animal. Animals receiving more than one treatment averaged costs exceeding 38 USD per animal. In total, 77% of all animals received the first treatment during the first 3 weeks after arrival. We found a relationship between shrink percentage and the distance traveled by the livestock from its origin to the feedlot (P = 0.049). BRD represents a costly condition for feedlot operations. It is imperative that cattleman and veterinary practitioners recognize the necessity to apply additional preventive medicine strategies to reduce the impact of BRD. Preconditioning and a more systematic use of methaphylaxis may improve our ability to accurately identify the most effective strategies to reduce the impact of BRD.

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Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle

Siedler

Roloff

Sá

et al. 2017

Protein Expression and Purification

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Bovine herpesvirus (BoHV) glycoprotein E (gE) is a non-essential envelope glycoprotein and the deletion of gE has been used to develop BoHV-1 and BoHV-5 differential vaccine strains. The DIVA (Differentiation of Infected from Vaccinated Animals) strategy, using marker vaccines based on gE-negative BoHV strains, allows the identification of vaccinated or infected animals in immunoassays designed to detect anti-gE antibodies. In this study a codon optimized synthetic sequence of gE containing highly conserved regions from BoHV-1 and BoHV-5 was expressed in Pichia pastoris. Following expression, the recombinant gE (rgE) was secreted and purified from the culture medium. The rgE was identified by Western blotting (WB) using sera from cattle naturally infected with BoHV-1 and/or BoHV-5, or sera from bovines experimentally infected with wild-type BoHV-5. Sera collected from cattle vaccinated with a BoHV-5 gI/gE/US9¯ marker vaccine failed to recognise rgE. Expression of rgE, based on a sequence containing highly conserved regions from BoHV-1 and BoHV-5, in P. pastoris enabled the production of large quantities of rgE suitable for use in immunoassays for the differentiation vaccinated or infected cattle.

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Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E

Cited by 8 publications

References 29 publications

Prokaryotic expression of a truncated form of bovine herpesvirus 1 glycoprotein E (gE) and its use in an ELISA for gE antibodies

Prokaryotic expression of a truncated form of bovine herpesvirus 1 glycoprotein E (gE) and its use in an ELISA for gE antibodies

Detection and economic impact related to bovine respiratory disease, shrink, and traveling distance in feedlot cattle in Northwest Mexico

Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle

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