Epidemiological survey on Mycoplasma gallisepticum and M. synoviae by multiplex PCR in commercial poultry

Buim, M. R.; Mettifogo, Elena; Timenetsky, Jorge; Kleven, S. H.; Ferreira, Antônio José Piantino

doi:10.1590/s0100-736x2009000700009

Cited by 52 publications

(54 citation statements)

References 10 publications

(8 reference statements)

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“…A recent study reports that the prevalence of MS and MG on farms in general is 72.7%. The results also indicate the dissemination of mycoplasmas among the farms surveyed, with an increase in the incidence of MS and a reduction in the incidence of MG among commercial poultry farms in Brazil (Buim et al 2009). Report that birds free of MS that are introduced onto a farm with a history of infection may become infected as well due to the perpetuation of the microorganism in the surrounding environment (Fiorentin et al 2002).…”

Section: Discussionmentioning

confidence: 67%

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Occurrence of Mycoplasma synoviae on commercial poultry farms of Pernambuco, Brazil

et al. 2014

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Pesq. Vet. Bras. 34(10):953-956, outubro 2014 953 RESUMO.-[Ocorrência de Mycoplasma synoviae em granjas comerciais da avicultura de Pernambuco.] O estado de Pernambuco é o maior produtor de ovos da região Norte e Nordeste e ocupa a segunda posição na produção de frangos de corte. Os micoplasmas são importantes patógenos aviários que causam doenças respiratórias e sinovite que resultam em grandes perdas econômicas.Objetivou-se pesquisar a ocorrência de Mycoplasma gallisepticum (MG) e Mycoplasma synoviae (MS) em frangos de corte e poedeiras comerciais no Estado de Pernambuco, Brasil. Foram colhidos fragmentos de traquéia de 55 frangos de corte sadios, 35 com sinais respiratórios e de 30 poedeiras comerciais também com sinais respiratórios, provenientes de 24 granjas, cada amostra foi composta por um "pool" de cinco aves. Para detecção de Mycoplasma gallisepticum (MG) e Mycoplasma synoviae (MS) foram utilizados o exame bacteriológico, PCR e Nested-PCR. Todas as amostras apresentaram resultados negativos no exame bacteriológico. Na PCR, sete amostras foram positivas para MS e uma para MG em amostras de aves com sinais respiratórios, sendo a amostra positiva para MG confirmada como cepa vacinal MG-F. A ocorrência de MS em aves com sinais clínicos respiratórios pode indicar ausência de barreiras sanitárias adequadas em granjas de frangos de corte e de poedeira comercial, favorecendo a sua propagação. The state of Pernambuco is the largest producer of eggs in the North and Northeast of Brazil and second one in the broiler production. Mycoplasmas are important avian pathogens, which cause respiratory and joint diseases that result in large economic losses. The aim of the present study was to investigate the occurrence of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in broilers and commercial laying hens in the state of Pernambuco, Brazil. Tracheal fragments were analyzed from 55 healthy broilers, 35 broilers with respiratory signs and 30 commercial laying hens with respiratory signs, from 24 commercial poultry farms, each sample was composed of a pool of five birds. The bacteriological exam, PCR and nested PCR were used for the detection of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS). All samples were negative in bacteriological isolation. In the PCR analyses, seven samples from birds with respiratory signs were positive for MS and one was positive for MG, the latter of which was confirmed as the MG-F vaccine strain. The occurrence of MS in chickens with respiratory signs may indicate inadequate sanitary management on poultry farms, favoring the propagation of mycoplasmosis. Occurrence of

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Section: Discussionmentioning

confidence: 67%

“…In Brazil, the frequency of infection by MS is greater than that by MG (Reis et al 1973, Mettifogo et al 2002, Buim 2005, Buim et al 2009). Moreover, the prevalence of MS in chickens has increased in the past twenty years (Balen & Fiorentin 1990).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Mycoplasma synoviae on commercial poultry farms of Pernambuco, Brazil

et al. 2014

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“…Previous studies showed high prevalence of MS and IBV in Brazil. Buim et al (2009) found that 55% (16/29) of layer flocks were positive to MS and none to MG by PCR, while Sandri et al (2008) found 43.5% (20/46) positive to IBV by RT-PCR.…”

Section: Resultsmentioning

confidence: 97%

Eggshell apex abnormalities in a free-range hen farm with mycoplasma synoviae and infectious bronchitis virus in Rio de Janeiro state, Brazil

Santos¹,

Brandão²,

Silva³

et al. 2014

Rev. Bras. Cienc. Avic.

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Eggs, mycoplasm, egg defects, egg abnormality, economic loss.Submitted: June/2013 Approved: March/2014 ABSTRACT A farm with 3,000 free-range hens between 24 and 65 weeks of age was investigated. These hens were separated in small flocks of 400 to 700 birds, presenting 10 to 23% egg production reduction. Twenty serum samples were collected during the period of drop in egg production and three weeks later for the investigation of Mycoplasma synoviae (MS), M. gallisepticum (MG) and Infectious Bronchitis Virus (IBV) antibodies using ELISA. At the time of the second collection, egg production had resumed to normal levels; however, with 10.23% of the eggs showed eggshell abnormalities limited to the apex. Eggshell strength was significantly different between normal and those with eggshell apex abnormalities, but not other egg-quality parameters. ELISA tests showed that MS and IBV titers increased during the evaluated period. MS infection was confirmed by culture and by PCR of tracheal swabs. All samples were negative for MG by ELISA and PCR. Further studies with larger samples to ensure the occurrence of this disease in industrial layer flocks in Brazil are under way.

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“…Embora psitacídeos desenvolvam frequentemente sinusite e conjuntivite, não são hospedeiros comuns e a patogenicidade de Mycoplasma nessas aves não está bem elucidada (Phalen et al 2006, Buim et al 2009). Consequentemente é importante sua investigação, já que a ave se torna portadora e imunossuprimida (Bradbury et al 1993), favorecendo ainda, o acometimento por outras enfermidades.…”

Section: Introducãounclassified

Pesquisa de Mycoplasma em aves da família Psittacidae mantidas em diferentes cativeiros no Brasil Central

et al. 2017

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RESUMO: O presente estudo teve como objetivo investigar a presença de Mycoplasma gallisepticum e M. synoviae em diferentes espécies de psitacídeos cativos no Brasil Central. Um total de 300 amostras foram coletadas e corresponderam a 41 espécies de psitacídeos da fauna brasileira, provenientes do CETAS, criadouro comercial e criadouro conservacionista. Quatorze espécies apresentaram amostras positivas para M. gallisepticum destacando a maracanã-verdadeira (Primolius maracana) (01/02, 50%), a arara-canindé (Ara ararauna) (15/48, 33,3%) e a jandaia-verdadeira (Aratinga jandaia) (03/10, 30%). Amostras do CETAS obtiveram total de 21,62% (16/74) de amostras positivas, do criadouro comercial 15,7% (19/121) e do criadouro conservacionista 6,66% (7/105). Apenas três espécies foram positivas para M. synoviae sendo essas, a maracanã-pequena (Primolius maracana) (1/10 - 10%), arara-macao (Ara macao) (1/12, 8,3%) e arara-canindé (Ara ararauna) (2/48, 4,1%). O CETAS obteve 2,7% (2/74) de amostras positivas totais, enquanto o criadouro conservacionista obteve total de 1,9% (2/105) de amostras. Não ocorreram amostras positivas para M. synoviae no criadouro comercial. Os resultados mostraram um considerável número de amostras positivas para M. gallisepticum em espécies da família Psittacidae, indicando que estes animais podem ser uma fonte de infecção silenciosa para outras aves, uma vez que não apresentaram sintomatologia clínica.

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Epidemiological survey on Mycoplasma gallisepticum and M. synoviae by multiplex PCR in commercial poultry

Cited by 52 publications

References 10 publications

Occurrence of Mycoplasma synoviae on commercial poultry farms of Pernambuco, Brazil

Occurrence of Mycoplasma synoviae on commercial poultry farms of Pernambuco, Brazil

Eggshell apex abnormalities in a free-range hen farm with mycoplasma synoviae and infectious bronchitis virus in Rio de Janeiro state, Brazil

Pesquisa de Mycoplasma em aves da família Psittacidae mantidas em diferentes cativeiros no Brasil Central

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