Neurotoxicose em bovinos associada ao consumo de bagaço de malte contaminado por Aspergillus clavatus

Bezerra, Pedro Soares; Raymundo, Djeison Lutier; Spanamberg, Andréia; Corrêa, Ana Grasielle Dionísio; Júnior, Jorge J. Bangel; Ferreiro, Laerte; Driemeier, David

doi:10.1590/s0100-736x2009000300006

Cited by 6 publications

(22 citation statements)

References 14 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Os achados clínicos e patológicos desses ovinos foram semelhantes aos descritos em bovinos e ovinos na doença neurológica espontânea associada com ingestão de alimentos contaminados por A. clavatus e reproduzida experimentalmente (Kellerman et al 1976, 1984, Gilmour et al 1989, Shlosberg et al 1991, Van der Lugt et al 2002, Loretti et al 2003, Colodel et al 2004, El-Hage & Lancaster 2004, McKenzie et al 2004, Bezerra Jr et al 2008. Os experimentos com os isolados de A. clavatus do bagaço de malte das propriedades evidenciam envolvimento do fungo nos surtos de bovinos.…”

Section: Discussionunclassified

“…Por outro lado, a semelhança nos tempos de início dos sinais clínicos e evolução clínica sugerem que a toxidez do subproduto da Propriedade 1 foi comparável à da cultura pura do fungo. Essa aparente superior toxicidade do subproduto da Propriedade 1 talvez possa estar associada com a maior letalidade observada naquele surto (Bezerra Jr et al 2008).…”

Section: Discussionunclassified

“…A distribuição da degeneração e necrose neuronal cromatolítica observada nesses casos foi semelhante à descrita previamente (Kellerman et al 1976, 1984, Gilmour et al 1989, Shlosberg et al 1991, Van der Lugt et al 2002, Loretti et al 2003, El-Hage & Lancaster 2004, McKenzie et al 2004, Colodel et al 2004, Bezerra Jr. et al 2008. Os neurônios envolvidos eram neurônios motores superiores e inferiores, sensórios e de gânglios autônomos, denotando uma doença neuronal degenerativa multissistêmica.…”

Section: Discussionunclassified

“…O bagaço de malte contaminado foi obtido em duas propriedades (Propriedades 1 e 2) onde ocorreram surtos confirmados de neurotoxicose causada por Aspergillus clavatus (Bezerra Jr et al 2008). Este subproduto foi administrado para três ovinos por via oral com seringa descartável de 60ml com a extremidade cortada.…”

Section: Methodsunclassified

“…Isolados de A. clavatus, obtidos do bagaço de malte das propriedades onde ocorreram os surtos da doença em bovinos (Loretti et al 2003, Bezerra Jr et al 2008, foram utilizados para reprodução experimental com cultura fúngica. Estes isolados, armazenados no Laboratório de Micologia da Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, em água destilada estéril, seguindo a técnica de Castellani (Diogo et al 2005), foram repicados em Agar Sabouraud e incubados por 10 dias, a temperatura de 25-30°C.…”

Section: Methodsunclassified

See 4 more Smart Citations

Intoxicação experimental por Aspergillus clavatus em ovinos

et al. 2009

Self Cite

View full text Add to dashboard Cite

205Pesq. Vet. Bras. 29(3):205-210, março 2009 RESUMO.-Descreve-se a reprodução experimental de doença neurológica em ovinos através da administração de bagaço de malte (resíduo cervejaria) contaminado com Aspergillus clavatus. Esse resíduo de cervejaria, cujas amostras revelaram cultura pura de A. clavatus, estava sendo utilizado em duas propriedades, onde ocorreram surtos da doença em bovinos. Os sinais clínicos iniciaramse cerca de 2 a 6 dias após a administração do subproduto ou da cultura e a evolução clínica foi de 1,5 a 12 dias. Os sinais clínicos, que foram predominantemente locomotores e respiratórios, incluíram tremores musculares, hiperestesia, taquipnéia progressiva, rigidez de membros pélvicos (mais evidente à locomoção), fraqueza dos posteriores e decúbito. Um ovino também apresentou apoio ocasional sobre os boletos dos membros pélvicos. As anormalidades locomotoras e tremores eram intensificados pelo exercício. Entretanto, em 6 dos 7 ovinos, o apetite e a dipsia eram mantidas até próximo à morte ou eutaná-sia. O principal achado histológico consistia de degeneração e necrose neuronal cromatolítica em núcleos nervosos específicos do tronco encefálico, cornos ventrais da medula espinhal e gânglios espinhais, trigeminal, estrelado e celíaco. Três ovinos também apresentaram degeneração e necrose leves em músculos dos membros pélvicos e torácicos. Samples of this by-product, in which pure cultures of A. clavatus grew, originated from two farms where outbreaks of A. clavatus poisoning in cattle had occurred. The onset of symptomatology was 2 to 6 days after dosage with the contaminated beer by-product or pure A. clavatus culture. The clinical course lasted from one and a half to 12 days. Clinical signs were predominantly of locomotor and respiratory nature and included muscle tremors, hyperesthesia, and progressive tachypnea, rigidity of the pelvic limbs, posterior weakness, and recumbency. One sheep also showed occasional knuckling of fetlocks of the hind limbs. Gait abnormalities and tremors were more pronounced after exercise. In 6 of 7 sheep, appetite and dypsia were maintained until close to death or euthanasia. The main histological findings consisted of chromatolytic neuronal degeneration and necrosis in selected nuclei of the brain stem, the ventral horn of the spinal cord and the spinal, trigeminal, stellate and celiac ganglions. Three sheep also presented slight degenerative and necrotic changes in muscles of the pelvic and thoracic limbs.

show abstract

Section: Discussionunclassified

Section: Methodsunclassified

See 3 more Smart Citations

Intoxicação experimental por Aspergillus clavatus em ovinos

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

Neurological diseases in cattle caused by plants and mycotoxins in Santa Catarina state, Brazil

et al. 2019

View full text Add to dashboard Cite

This study described the epidemiological and clinical-pathological aspects of 25 outbreaks of neurological diseases in cattle caused by plants and mycotoxins in Santa Catarina state. Six of them were due to Sida carpinifolia poisoning, five to Solanum fastigiatum, five to Phalaris angusta, three to Claviceps paspali, three to Claviceps purpurea, and three outbreaks were of unknown etiology. The clinical signs observed in the affected cattle were mild to severe and characterized by generalized muscle tremors, incoordination, hypermetria, wide-based stance, intentional head tremors, dull staring eyes, and frequent ear twitching, with convulsions in some cases. At necropsy, lesions were observed only for P. angusta poisoning, characterized by gray-greenish discoloration in thalamus and midbrain. Microscopically, rarefaction and/or disappearance of Purkinje neurons with substitution by Bergmann cells were observed for S. carpinifolia and S. fastigiatum poisoning. For P. angusta poisoning, thin granular brown-yellowish pigment was observed in the cytoplasm of some neurons. Gross and microscopic findings were not observed in three outbreaks of tremorgenic disease of unknown etiology. Experiments conducted with leaves, flowers and seeds of Ipomoea indivisa and Ipomoea triloba, as well as with maize and soybean residues contaminated with Ipomoea spp. did not reproduced clinical signs.

show abstract

Tremorgenic syndrome caused by Ipomoea pes caprae in cattle

et al. 2020

View full text Add to dashboard Cite

Poisonous plants are a significant cause of death among adult cattle in Brazil. Plants that affect the central nervous system are widely spread throughout the Brazilian territory and comprise over 30 toxic species, including the genus Ipomoea, commonly associated with a lysosomal storage disease and a tremorgenic syndrome in livestock. We describe natural and experimental Ipomoea pes caprae poisoning in cattle from a herd in the Northside of Rio de Janeiro, Brazil. Affected cattle presented episodes of severe ataxia, abnormal posture followed by falling, muscular tremor, contraction, and spasticity, more prominent in the limbs, intensified by movement and forthcoming, and recumbence. Grossly, a substantial amount of leaves and petioles were found in the rumen. Histopathological examination showed degenerative neuronal changes, mostly in cerebellar Purkinje cells, which were confirmed with Bielschowsky silver. The characteristic clinical changes and mild histological lesion strongly suggested a tremorgenic syndrome. Lectin- immunohistochemistry evaluation reinforced this hypothesis; all lectins tested failed to react with affect neurons and Purkinje cells, which ruled out an underlying lysosomal storage disease. One calf given I. pes caprae leaves experimentally developed clinical signs similar to natural cases. On the 28th day of the experiment, the plant administration was suspended, and the calf recovered within four days. I. pes caprae’s spontaneous tremorgenic syndrome in cattle is conditioned to exclusive feeding for several months. We were able to experimentally reproduce toxic clinical signs 12 days following the ingestion.

show abstract

Neurotoxicose em bovinos associada ao consumo de bagaço de malte contaminado por Aspergillus clavatus

Cited by 6 publications

References 14 publications

Intoxicação experimental por Aspergillus clavatus em ovinos

Intoxicação experimental por Aspergillus clavatus em ovinos

Neurological diseases in cattle caused by plants and mycotoxins in Santa Catarina state, Brazil

Tremorgenic syndrome caused by Ipomoea pes caprae in cattle

Contact Info

Product

Resources

About