Genes associated with pathogenicity of avian Escherichia coli (APEC) isolated from respiratory cases of poultry

Rocha, Ângela Sanches; Rocha, Silvio L.S.; Lima-Rosa, C. A. V.; Souza, Guilherme Fonseca de; Moraes, H. L. S.; Salle, F. O.; Moraes, Lucas Brunelli de; Salle, Carlos Tadeu Pippi

doi:10.1590/s0100-736x2008000300009

Cited by 27 publications

(29 citation statements)

References 27 publications

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“…Em estudo feito por roCha et al (2008), aves com sintomatologia respiratória e lesões compatíveis com colibacilose apresentaram um maior percentual do gene de adesão Fel A (24/61) em relação ao gene Pap C (15/61), este úl-timo envolvido na colonização de órgãos sistêmicos (PourbaKhsh et al, 1997). A ausência do gene Fel A de E. coli esteve associada ao decréscimo de aerossaculite, porém, com probabilidade pequena e não significativa, o que pode ser explicado pela falta de ação concomitante dos outros fatores de virulência de E. coli, de vez que a ação da referida bactéria depende do impacto cumulativo de vários fatores de virulência roCha et al, 2008;sKyberG et al, 2003). Microbiology, v.145, p.297-309, 1995. DOZOIS, C.M.…”

Section: Discussionunclassified

“…Sua especificidade para receptores das células do hospedeiro capacita as bactérias à superação dos mecanismos fisiológicos de defesa, permitindo a colonização e multiplicação com ou sem invasão sistêmica subsequente. Os genes de adesão comumente identificados em estirpes de E. coli isoladas de aves são a fímbria P (pap C) e fímbria F11 (Fel A) (la raGione; WooDWarD, 2002;roCha et al, 2008). PourbaKhsh et al (1997) Os "pools" de traqueias de cada lote foram acondicionados em placas de Petri esterilizadas.…”

Section: Introductionunclassified

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PCR na detecção de gene Fel A de Escherichia Coli em frangos de corte condenados por aerossaculite pela Inspeção Sanitária Federal

et al. 2013

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A colibacilose é considerada uma das principais doenças da indústria avícola moderna, devido aos grandes prejuízos econômicos causados. A Escherichia coli contribui não só para a doença em si, levando à perda de peso das aves, bem como para o aumento da taxa condenação de carcaças durante o abate e processamento. A detecção de fatores de virulência de cepas de E. coli do patotipo APEC colabora para a caracterização de sua patogenicidade e as técnicas de PCR têm sido muito úteis na pesquisa de genes que os codificam. Este estudo objetivou diagnosticar E. coli pela detecção o gene Fel A por PCR e relacionar a positividade para este agente com o baixo peso em frangos de corte provenientes de lotes condenados por aerossaculite. Foram estudados 40 lotes de frangos de corte abatidos em um matadouro avícola sob Inspeção Sanitária Federal, localizado no Estado do Rio Grande do Sul. Foram colhidos aleatoriamente 3 frangos e obtidos "pools" de três traqueias em cada um deles para PCR. O DNA foi extraído pelo método de fenol-clorofórmio e amplificado com pares de "primers" específicos para gene Fel A de E. coli. Dos 40 lotes analisados pela PCR, 35% (14/40) foram positivos para o gene Fel A. A PCR foi eficaz para a detecção do gene Fel A em lotes de frangos de corte e houve relação entre a presença do gene Fel A, a queda de peso e aumento na taxa de aerossaculite.

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Section: Discussionunclassified

Section: Introductionunclassified

PCR na detecção de gene Fel A de Escherichia Coli em frangos de corte condenados por aerossaculite pela Inspeção Sanitária Federal

et al. 2013

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“…A variation in the prevalence of ColV plasmids of 99.1% and 66.8% were reported among APEC isolates in the UK and USA, respectively (McPeake et al, 2005;Rodriguez-Siek et al, 2005a). While a lower frequency was documented in Brazil (22%) and Spain (23%) (Blanco et al, 1997;Rocha et al, 2008). …”

Section: Colicinsmentioning

confidence: 99%

“…Serum resistance VGs enable the bacteria to survive outside the gastrointestinal tract and overcome the host defence mechanisms (Johnson et al, 2002b;Lynne et al, 2007;Miajlovic and Smith, 2014;Rocha et al, 2008;Tivendale et al, 2004). These VGs are frequently associated with septicaemia as they assist the bacteria in avoiding being killed by the essential defence mechanisms present in the blood, including complement, antimicrobial peptides and other serum components (Boerlin and Reid-Smith, 2008;Jacobson et al, 1992;Jeffrey et al, 2002;Nolan et al, 2003).…”

Section: Serum Resistancementioning

confidence: 99%

“…These VGs are frequently associated with septicaemia as they assist the bacteria in avoiding being killed by the essential defence mechanisms present in the blood, including complement, antimicrobial peptides and other serum components (Boerlin and Reid-Smith, 2008;Jacobson et al, 1992;Jeffrey et al, 2002;Nolan et al, 2003). Several investigations have reported serum resistance VGs are widespread among APEC in comparison with AFEC and have suggested that these VGs contribute to APEC pathogenicity (Delicato et al, 2003;Dozois et al, 1992;Dziva and Stevens, 2008;Lynne et al, 2007;Mellata et al, 2003;Mellata et al, 2012;Nolan et al, 2002;Ozawa et al, 2008;Rocha et al, 2008;Rodriguez-Siek et al, 2005a;Skyberg et al, 2008;Tivendale et al, 2004;Wooley et al, 1992;Yaguchi et al, 2007;Yang et al, 2004;Zhao et al, 2005). Several VGs have been linked with serum resistance mechanisms and the association of each of the serum resistance VGs with APEC pathogenicity mechanisms have also been independently studied (Nakazato et al, 2009;Rocha et al, 2008).…”

Section: Serum Resistancementioning

confidence: 99%

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Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Virulence genotyping of Escherichia coli isolates from avian cellulitis in relation to phylogeny

2009

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Genes associated with pathogenicity of avian Escherichia coli (APEC) isolated from respiratory cases of poultry

Cited by 27 publications

References 27 publications

PCR na detecção de gene Fel A de Escherichia Coli em frangos de corte condenados por aerossaculite pela Inspeção Sanitária Federal

PCR na detecção de gene Fel A de Escherichia Coli em frangos de corte condenados por aerossaculite pela Inspeção Sanitária Federal

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Virulence genotyping of Escherichia coli isolates from avian cellulitis in relation to phylogeny

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