Análise filogenética de papilomavírus bovino associado com lesões cutâneas em rebanhos do Estado do Paraná

Abstract

analysis analysis analysis analysis analysis of bovine papillomavirus associated with skin warts in cattle herds from the of bovine papillomavirus associated with skin warts in cattle herds from the of bovine papillomavirus associated with skin warts in cattle herds from the of bovine papillomavirus associated with skin warts in cattle herds from the of bovine papillomavirus associated with skin warts in cattle herds from the state of Bovine papillomavirus (BPV) infection causes hyperplastic lesions in the cutaneous epithelium of cattle. Six types of BPV were classified in two sub-groups, being correlated to the anatomical regions of the infection and morphologic characteristics of the lesions. The present study was carried out to identify the types of BPV present in skin warts of cattle from the state of Paraná, Brazil. The generic primers FAP59 and FAP64 were used for amplification of a 478 bp fragment of BPV L1 gene in nine cutaneous papilloma samples obtained from six animals in four herds. In all papillomas examined, a product with the expected molecular size was amplified. Phylogenetic analysis of the PCR products identified BPV-2 in three samples, BPV-1 in one, and BPV-6 in five papillomas. BPV-6 was detected in cutaneous papillomas of the teat and in other body parts as well. In one animal, from which more than one sample was collected, a concomitant infection by BPV-1 and BPV-2 was identified. The five positive BPV-6 samples showed a nucleotide identity of 100% with the sequence of the reference strain available in GenBank. However, differences among BPV-2 and BPV-1 Brazilian samples and the respective reference sequences deposited in GenBank were observed. Molecular comparison of the two BPV-2 strains identified showed the involvement of two viral variants. This study revealed the diversity of BPV types circulating in the state of Paraná.INDEX TERMS: Bovine papillomavirus, cutaneous papillomatosis, phylogeny, polymerase chain reaction.Pesq. Vet. Bras. 27(7): 314-318, julho 2007 Análise filogenética do papilomavírus bovino associado a lesões cutâneas em rebanhos do Estado do Paraná 315

Detection of Bovine Papillomavirus Types, Co-Infection and a Putative New BPV11 Subtype in Cattle

“…In India and Germany, different BPV types were identified in the same lesion (Pangty et al, 2010;Schmitt et al, 2010). In Brazil, the presence of BPV types 1, 2, 4 and 6 in the same lesion was also reported (Yaguiu et al, 2006;Claus et al, 2007Claus et al, , 2009bLindsey et al, 2009).…”

Ultrastructural Study of Bovine Papillomavirus During Outbreaks in Brazil

“…Maeda et al reported to have found only in the teats of heifers. It has already been reported the appearance of lesions in the abdomen, shoulder, chest, flank, base of the horns, eyes, ears, tongue, dewlap, neck, jaw, hip, thigh, buttocks, vulva, rectum, knees and posterior back of cattle and buffaloes (Tweddle & White, 1977;Cappellaro et al, 1978;Lancaster & Olson, 1982;Nooruddin et al;Julinek & Tochezy, 2005;Turk et al;Claus et al, 2007;Özsoy et al;Zhu et al;Lunardi et al, Ford et al).…”

Papillomaviruses in ruminants: An update

“…The typical pedunculate form presents verrucous aspect, known as cauliflower form. Papillomatous lesions are found in head, neck, dorse, abdomen, udder, teat and mucosa of digestive and genital tracts (Batista et al., ; Borzacchiello & Roperto, ; Claus, Vivian, Lunardi, Alfieri, & Alfieri, ; Lunardi et al., ) (Table ). Virtually all BPVs have been detected in cutaneous lesions, even the PVs which are found in malignant lesions, as the Delta‐PVs represented by BPVs 1, 2, 13 and 14 (Table ).…”