Diagnóstico sorológico da brucelose bovina em animais adultos vacinados com dose reduzida da cepa 19 de Brucella abortus

Jardim, Gustavo Coelho; Pires, Pedro; Mathias, Luís Antônio; Ribeiro, Olímpio Crisóstomo; Kuchembuck, Márcio Rubens Graf

doi:10.1590/s0100-736x2006000300009

Cited by 12 publications

(13 citation statements)

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“…Based on microbiological culture, phenotypic characteristics and PCR analysis, these five isolates were identified as B. abortus strain 19. Positive serodiagnosis, SPA, and modified 2-ME in five bovine bulls from Brazil that shedding B. abortus strain 19 in the semen probably was caused by vaccination of male calves or through exposure to a strain 19-related abortion by an adult cow [23,24]. It is important to note that in Brazil, this vaccine is approved exclusively for heifers (3e8 months of age) [24].…”

Section: Resultsmentioning

confidence: 99%

“…Positive serodiagnosis, SPA, and modified 2-ME in five bovine bulls from Brazil that shedding B. abortus strain 19 in the semen probably was caused by vaccination of male calves or through exposure to a strain 19-related abortion by an adult cow [23,24]. It is important to note that in Brazil, this vaccine is approved exclusively for heifers (3e8 months of age) [24]. In addition, the mechanism of B. abortus infection of bovines by B19 vaccine strain shedding in environment by females remains not entirely clear [25].…”

Section: Resultsmentioning

confidence: 99%

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Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls

et al. 2017

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Brucellosis remains as a major infectious disease of domestic animals and is considered a re-emerging zoonosis in several countries. B. abortus infections in bulls are related to reproductive tract infections, although infected animals show transient serological titers or nonreactor status. Thus, diagnosis of bovine brucellosis based exclusively on serological tests probably underestimates B. abortus infections in bulls. In this scenario, three hundred thirty-five serum samples from reproductively mature bovine bulls were subjected simultaneously to standard serodiagnosis using the rose Bengal test (RBT), 2-mercaptoethanol (2-ME), complement fixation (CFT), and fluorescence polarization assay (FPA). Furthermore, conventional semen plasma agglutination (SPA) and modified 2-ME, FC and, FPA were carried out in all bulls replaing serum by seminal plasma. Semen from all bulls was also analyzed for sperm viability, microbiological culture in Farrell media, and polymerase chain reaction (PCR). Only eight (2.38%) semen samples were considered improper for reproduction services (necrospermia and azoospermia), although none of these animals was positive in any of the diagnosis methods used. Five bulls (1.49%) were simultaneously positive in conventional RBT, 2-ME, SPA, modified 2-ME, microbiological culture in Farrell media, and in PCR for B. abortus strain 19. Two (1.67%) bulls were positive in PCR for B. abortus field strains and negative in all other tests, although semen was considered viable to reproduction service. The identification of B. abortus B19 strain in serum and semen of bulls occurred probably due to improper vaccination of males or infection by B19 strain shedding by vaccinated females that could to contaminated environment of farms. In addition, detection of B. abortus field strains only using PCR in bulls without sperm viability abnormalities indicate the need for including molecular methods to improve diagnosis of the disease in bovine bulls.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls

et al. 2017

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“…Baixa especificidade dos testes convencionais em bezerras bovinas vacinadas tem sido relatada (MATHIAS et al, 2001;JARDIM et al, 2006). Como esperado, o AAT foi o teste com menor especificidade aos 30 dias de vacinação, uma vez que nenhum animal apresentou resultado negativo.…”

Section: Discussionunclassified

RESPOSTA SOROLÓGICA DE BEZERRAS TABAPUÃS VACINADAS COM CEPA B19 de Brucella abortus

Blankenheim

Silva

Santos

et al. 2015

Ars Vet

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RESUMOFoi avaliada a resposta sorológica induzida pela vacinação com a dose padrão de 60-120x10 9 organismos da cepa B19 de Brucella abortus em bezerras Tabapuãs, entre 3 e 8 meses de idade, pelos testes sorológicos preconizados pelo Programa Nacional de Controle e Erradicação da Brucelose e Tuberculose (PNCEBT) para diagnóstico da brucelose bovina. Foram examinadas amostras de soro sanguíneo de 72 animais em quatro provas sorológicas: antígeno acidificado tamponado (AAT), teste de soroaglutinação lenta juntamente com o teste de 2-mercaptoetanol (2-ME), reação de fixação de complemento (RFC) e teste de polarização fluorescente (TPF), o qual foi interpretado de duas formas: ponto de corte variável e ponto de corte fixo. As amostras de soro sanguíneo foram colhidas imediatamente antes da vacinação, e após 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 e 360 dias. Foi calculada a especificidade dos testes e os resultados foram comparados pelo indicador kappa. O TPF apresentou especificidade mais elevada do que os outros testes nas amostras colhidas entre 30 e 90 dias após a vacinação. Aos 270 dias de vacinação, observou-se especificidade de 93,74% no AAT, no 2-ME e na RFC. No TPF com ponto de corte variável a especificidade foi 87,5% e com ponto de corte fixo foi 100,0%. O TPF apresentou maior capacidade de discriminação dos títulos de anticorpos vacinais logo após a vacinação, quando comparado com os outros testes preconizados pelo PNCEBT, mostrando-se um teste útil para o diagnóstico da brucelose bovina. PALAVRAS-CHAVE:Brucelose, Polarização Fluorescente, Sorologia, Vacinação. SUMMARYThe study aimed to analyze the antibody response induced by vaccination with the standard dose of 60-120x10 9 organisms of Brucella abortus strain 19 in Tabapuã heifers, aged between 3 and 8 months, by serological tests recommended by the National Program for Brucellosis and Tuberculosis Control and Eradication (PNCEBT) for the diagnosis of bovine brucellosis. Serum samples of 72 animals were examined by four serological tests: rose Bengal test (RBT), standard agglutination test with the 2-mercaptoetanol (2-ME) test, complement fixation test (CFT) and fluorescence polarization assay (FPA), which was interpreted in two ways: variable cutoff and fixed cutoff. Heifers were bled immediately prior to vaccination, and after 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 days. Specificity of the tests was calculated, and the results were compared using the kappa statistic. FPA had a higher specificity than the other tests on the samples collected from 30 to 90 days after vaccination, but over time this difference decreased. At 270 days of vaccination, the specificity of RBT, 2-ME and RFC was 93.74%. The specificity of the FPA with variable cutoff was 87.5%, and with fixed cutoff was 100.0%. It was concluded that FPA had a higher discrimination ability for vaccination antibody titers shortly after vaccination, when compared with other tests recommended by the PNCEBT, and is a useful tool for the diagnosis of bovine brucellos...

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“…Entretanto, essa técnica possui sensibilidade limitada, alto custo e execução demorada. Diante disso, os métodos indiretos baseados em testes sorológicos são amplamente utilizados em programas de controle e erradicação da doença (Jardim et al, 2006).…”

Section: Diagnósticounclassified

“…As medidas de prevenção e controle para brucelose em bovinos e bubalinos se apóiam em dois pilares: vacinação e diagnóstico, que permitem reduzir ou evitar a exposição dos animais ao agente e aumentar a resistência do rebanho às brucelas (Jardim et al, 2006). Com isso, o Plano Nacional de Controle e Erradicação da Brucelose e Tuberculose (PNCEBT) determina a vacinação obrigatória de fêmeas entre três e oito meses e de animais adultos que não foram vacinados quando jovens, além de abate sanitário dos sororreagentes ao AAT e confirmados no 2-ME ou FC, controle do trânsito de animais, feito somente com certificado de teste negativo, interdição de propriedades com focos de brucelose e certificação de propriedades livres e monitoradas (Brasil, 2006 (Brasil, 2004).…”

Section: Diagnósticounclassified

Fatores associados à prevalência sorológica de Brucella ovis e Neospora caninum no rebanho ovino de Sergipe

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Diagnóstico sorológico da brucelose bovina em animais adultos vacinados com dose reduzida da cepa 19 de Brucella abortus

Cited by 12 publications

References 9 publications

Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls

Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls

RESPOSTA SOROLÓGICA DE BEZERRAS TABAPUÃS VACINADAS COM CEPA B19 de Brucella abortus

Fatores associados à prevalência sorológica de Brucella ovis e Neospora caninum no rebanho ovino de Sergipe

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