Um protocolo de "nested-PCR" para detecção do vírus da anemia das galinhas

Simionatto, Simone; Lima-Rosa, Carlos André da Veiga; Rubin, Lauricio Librelotto; Canal, Cláudio Wageck

doi:10.1590/s0100-736x2005000200007

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Dna Amplification and Sequencing1

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2011

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Cited by 2 publications

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References 26 publications

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“…Ao comparar a qualidade do produto extraído e amplificado por nested-PCR em gel de sacarose, SIMIONATTO et al (2005) verificaram que o produto obtido por CTAB apresentava qualidade de nitidez (sem a formação de bandas inespecíficas) superior à do obtido pelo método com dodecil sulfato de sódio, e qualidade semelhante à do produto extraído pelo método de fenol-clorofórmio, porém com menos intensidade comparando-se com o método de extração com tiocinato de guanidina.…”

Section: Resultsunclassified

COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLAS

et al. 2011

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Section: Resultsunclassified

COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLAS

et al. 2011

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“…The DNA extraction methods, primers and cycling conditions for the Nested-PCR assay were performed as described previously [25]. The 539 bp amplification products were purified with GFX PCR DNA and Gel Band Purification Kit (Amersham Biosciences, Sweden).…”

Section: Dna Amplification and Sequencingmentioning

confidence: 99%

Characterization and Phylogenetic Analysis of Brazilian Chicken Anaemia Virus

et al. 2006

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Chicken anaemia virus (CAV) was detected by a Nested-PCR assay in field samples from different regions of Brazil. The 539 bp amplified fragments of vp1 gene from 44 field samples were sequenced and 10 new nucleotide sequences of CAV were observed. These sequences were phylogenetically analysed by Mega2 using neighbour joining distance methods with 1000 bootstrap replications. Phylogenetic analysis did not show correlation between CAV pathology pattern and genetic groups. The 10 nucleotide sequences of the Brazilian samples were also analysed together with 30 sequences of CAV strains previously described from other countries. The genetic variability observed was not related to the geographical distribution. Amino acid substitutions were detected at 9 positions of the Brazilian sequences and two of them had not been observed before, (65)R replacing the Q residue and (98)F replacing Y residue.

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Um protocolo de "nested-PCR" para detecção do vírus da anemia das galinhas

Abstract: ABSTRACT ABSTRACT ABSTRACT.-Simionatto S., Lima-Rosa C

Cited by 2 publications

References 26 publications

COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLAS

COMPARAÇÃO DE MÉTODOS PARA EXTRAÇÃO DE DNA NA REAÇÃO EM CADEIA DA POLIMERASE PARA DETECÇÃO DE Salmonella enterica SOROVAR Enteritidis EM PRODUTOS AVÍCOLAS

Characterization and Phylogenetic Analysis of Brazilian Chicken Anaemia Virus

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