The present study aimed at assessing the effects of methionine and arginine on the immune response of broiler chickens submitted to immunological stimuli. Three methionine concentrations (0.31, 0.51, and 0.66% from 1 to 21 days of age; 0.29, 0.49, and 0.64% from 22 to 42 days of age) and 2 arginine concentrations (1.33 and 1.83%; 1.14 and 1.64% for the same life periods) were tested. Birds were divided into two groups for immunological stimuli (3x2x2 arrangement). Vaccines against Marek's disease, fowl pox, infectious bronchitis, Freund's Complete Adjuvant, Sheep red blood cells (SRBC), and avian tuberculin were administered to one group as immunological stimuli; the other group did not receive any stimulus. The experiment was carried out with 432 one-day-old male Ross broilers, distributed into 12 treatments with 6 replicates of 6 birds each. Performance data were weekly collected. Anti-SRBC antibodies were collected by hemagglutination test and cell immune response (CIR) was measured by tubercularization reaction in one wattle 24 hours after administration of the second tuberculin injection at 42 days of age. The weight difference between the two wattles of each bird (one injected with tuberculin and the other not) was the measure of CIR. Arginine levels did not influence either bird performance or immune response. Methionine concentrations higher or lower than usually adopted in broiler production (0.51 and 0.49%) equally failed to influence the birds' immune humoral response, but the best CIR was observed at the intermediate methionine level. Vaccines administered on the first day of age impaired bird performance up to the 21st day of age

Four Nutritional Programs (NP) used in the Brazilian poultry industry were tested in two broiler strains (Cobb 500 and Ross 308). NP varied in the concentrations of their main essential amino acids (AA) and were classified as Low, Regular, High and Mixed (high AA concentrations up to 21 days and regular concentrations after that). Minimum digestible Met+Cys/Lys, Thr/Lys, Arg/Lys, Ile/Lys, and Val/Lys ratios were 0.74, 0.64, 1.05, 0.65 and 0.75, respectively, in all NP, and no minimum amount of CP was fixed. There were no interactions between strain and NP for any of the evaluated responses. From 1 to 47 days of age, birds fed the Low NP presented lower average body weight and body weight gain (BWG). The high NP allowed for better feed conversion ratio (FCR), followed by the Regular and the Mixed NP. Ross 308 broilers were heavier, presenting worse FCR due to higher FI. Birds fed the High NP had lower carcass yield than those fed the Low NP. The Low and Regular NP had lower costs per WG when compared with the High NP. Low and Regular NP presented higher gross margin returns compared with the High NP. The Regular and Mixed NP are the most recommended, presenting intermediate performance and higher economic returns.

Several changes in amino acid levels occur during an infection. As a rule, glycine, taurine and serine levels diminish considerably, while cysteine levels increase. Such changes may be attributed to the intense consumption of sulfur amino acids (SAA- methionine+cysteine) during infectious challenge. Methionine plays an important role in humoral and cellular immune responses. It has been suggested that such effect is exerted by intracellular glutathione and cysteine levels. Four-hundred thirty-two day-old Ross male broiler chickens were fed (from 1 to 42 days of age) three SAA levels in the diet (0.72, 0.82, and 0.92% from 1 to 21 days of age; 0.65, 0.75, and 0.85% from 22 to 42 days of age) and submitted to two immunological stimulus series. Vaccines against Marek's disease, Fowlpox, Infectious Bronchitis and Infectious Bursal disease, Freund's Complete Adjuvant, and avian tuberculin were used as immunological stimuli. The experiment comprised 6 treatments, with 6 replications using 12 birds per replicate. Performance data were collected weekly. Gumboro antibodies were measured by ELISA, and the cellular immune response by the tuberculin test. SAA levels tested did not influence immune response. Nevertheless, the vaccines applied on the 1st day impaired chick performance up to the 21 days of age. The SAA levels generally adopted in poultry husbandry may not be enough to assure weight gain, especially when birds are raised if a low-challenge infectious environment

ABSTRACT ABSTRACT.-Simionatto S., Lima-Rosa C

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