Desenvolvimento de uma prova de imunoadsorção enzimática para detecção de anticorpos contra Babesia bovis

Abstract

An enzyme-linked immunosorbent assay (ELISA) for antibodies to Babesia bovis was developed and evaluated in comparison with the indirect fluorescent antibody test (IFAT). The ELISA sensitivity and specificity, estimated with 100 positive sera from cattle experimentally infected with B. bovis and 108 negative sera collected from B. bovis-free herds, were 98.0% and 98.1%, respectively. Positive and negative predictive values were, respectively, 98.0% and 98.1%, and precision was 98.1%. No cross-reactions were detected with 80 sera from calves experimentally inoculated with Babesia bigemina. The ELISA was compared with IFAT using 110 cattle sera from an enzootically stable area and with 168 cattle sera from an enzootically unstable area. In both cases, there was a significant agreement between results of both tests (P=0.631 and 0.4725, respectively). In an epidemiological study performed with ELISA in the Pantanal region of the State of Mato Grosso do Sul with 1,365 cattle sera, 83.9% were positive for antibodies against B. bovis, characterizing this region as enzootically stable.INDEX TERMS: Babesia bovis, ELISA, indirect fluorescent antibody test, cattle, epidemiological survey, Pantanal, Mato Grosso do Sul, Brazil.

Soroprevalência e fatores de risco para Babesia bovis em rebanhos leiteiros na região sul de minas gerais

“…Embora apresentando pequenas variações na taxa de bovinos soropositivos para B. bovis em relação a outros estudos, em razão das características epidemiológicas próprias de cada região, a situação de estabilidade endêmica para B. bovis determinada no presente trabalho é similar à relatada em pesquisas realizadas em Minas Gerais (PATARROYO et al, 1987) e nos estados de São Paulo (BARCI et al, 1995), Bahia (ARAÚJO et al, 1997), Rio de Janeiro (SOARES et al, 2000), Mato Grosso do Sul (MADRUGA et al, 2000) e Goiás (SANTOS et al, 2001).…”

Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil

“…Persistent presence of ticks throughout the year allows continuous inoculation of the pathogens into animals from the time of their birth onwards, which allows them to develop specific immunity that is sufficient to make them resistant (FOLLY et al, 2009). Thus, calves have higher prevalence of agents for the BBA complex, as observed by Madruga et al (2000) in Mato Grosso do Sul, even without evidence of clinical signs (ZAUGG et al, 1986).…”

Serological survey of Babesia bovis, Babesia bigemina, and Anaplasma marginale antibodies in cattle from the semi-arid region of the state of Bahia, Brazil, by enzyme-linked immunosorbent assays

“…Detection of antibodies against B. bovis and B. bigemina -The indirect ELISA with crude antigen of B. bovis and B. bigemina were performed according to the techniques described by Madruga et al (2000bMadruga et al ( , 2001, respectively.…”