Caracterização das proteínas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transgênicos

Abstract

RESUMO.-A utilização da transgenia com a proteína fluorescente verde (GFP) como marcador de células de origem fetal nas placentas de clones bovinos servirá de modelo inédito para estudo morfofisiológico e imunológico da interação materno-fetal, visto que possibilitará o seu mapeamento, diferenciando as células fetais das maternas. TalCaracterização das proteínas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transgênicos The transgenic application of green fluorescent protein (GFP) as fetal cell marker on cattle cloned placenta could provide an exclusive model for studying the morphologic and immunologic maternal-fetal interactions, providing information about its mapping, distinguishing the fetal from maternal cells. This model will have direct application, mainly because these animals present problems during its development. With this model's support, we intend to verify the substances transport between mother and fetus during endocytosis, through the immunolocalization of protein named caveolae. For these, we used 06 cloned bovine and 30 cattle samples of artificial insemination (AI) with 90 days of pregnancy, which had been their development interrupted by humanitarian slaughter of the recipient and recovery of the pregnant uterus. We collected the placentome and the chorion. A part of the samples was cut and fixed, by immersion, on a solution containing 4% of parafomaldehyde or 10% of formaldehyde on a sodium phosphate buffer (PBS), at 0,1M pH 7.4, Zamboni solution (4% of paraformaldehyde, 15% of picric acid, on sodium phosphate buffer 0,1M pH 7.4), metacarn (60% of metanol, 30% of chloroform, and 10% glacial acetic acid), for morphologic and immunohistochemistry verification for caveolinas proteins -1 and -2 (CAV -1 and CAV-2). The caveolins -1 were found in fetal and maternal villi, but its strongest staining was observed in the endometrial stroma. The caveolins -2 had positive staining in trophoblast and chorioallantoic membrane, and specifically in giant trophoblastic binucleated cell. Therefore the results were compared between cloned cattle and from AI or natural mating, for assisting on detection of the reason of many placental alterations, embryonic losses, spontaneous abortion, post-natal mortality and large offspring syndrome on laboratory-manipulated animals. The result suggests that the proteins caveolins -1 and -2 (CAV-1 and CAV-2) are part of the caveolae composition and important structures related to the molecule transfer to the fetus, nourish it through endocytosis and pinocytosis.INDEX TERMS: Caveolin-1 and -2 proteins, caveolae, placentation, immunolocalization, cattle.

Characterization of the extracellular matrix in the chorioallantoic membrane of water buffalo (Bubalus bubalis) in early gestation

“…We also used as reference the studies of Abdel-Raouf and uni-and binucleate giant trophoblastic cells. Oliveira, McClellan, & Hansen (2010) observed in bovines that binucleate cells and blood vessels are present throughout all the gestational phases agreeing with the results of Miglino et al (2007) and Peres et al (2015) that also verify that the histological structures of the placenta and foetal membranes are very similar throughout the different gestational stages, these results being similar to those found in the initial third of gestation of buffalo. Bosch, 2006 described these binucleate cells being rounded or spherical cells of various sizes that evolve into binucleate giant cells.…”

Certificações, Padrões E Códigos: Instrumentos Voluntários Para Análise De Projetos Urbanos Sustentáveis, Um Balanço Dos Critérios Do Casbee No Contexto Brasileiro

“…O primeiro remete à doutrina de Otto Mayer, que assume ser possível à Administração Pública atuar como Estado ou como um particular, ou seja, submeterse ao regime privado integralmente, o que, conforme veremos a seguir, não é possível 23 .…”

“…at -4 °C to be dissected in saline solution (NaCl 0.215 mM). The first and last segments were removed to extraction of the midgut, which were fixed in modified Zamboni solution (10% formaldehyde, 15% picric acid in 0.1 μM sodium phosphate buffer) for 24 h at 4 °C [23]. Then, they were washed in 10% neutral buffered formalin (1 M of formaldehyde, 33 mM of sodium phosphate monobasic, 46 mM of dibasic sodium phosphate) at 4 °C until complete removal of the picric acid.…”

“…Fonte: KUCZYNSKI, E. Suicídio na Infância e Adolescência. Psicologia USP, v. 25, n. 3, p. 246-252, 2014. Estima-se que 800.000 pessoas morrem anualmente por suicídio, o que representa 1,4 % de mortes em todo o mundo, sendo registrado no ano de 2015 a prevalência no sexo feminino 0,00/100.000 habitantes (1-59 meses), 0,92/100.000 habitantes (5-14 anos de idade) e de 10,34/100.000 habitantes (15-29 anos de idade) e no sexo masculino 0,00/100.000 habitantes (1-59 meses), 1,10/100.000 habitantes (5-14 anos de idade) e de 14,06/100.000 (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29) O ciclo de vida humana perpassa por constantes modificações diárias sejam elas de ordem físicas ou mentais, as quais constitui as fases do desenvolvimento psíquico e cronológico. Desse modo, tem-se as fases etárias e psíquicas do desenvolvimento humano de criança, de adolescência, de adulto e de idoso.…”