Efeito da pentoxifilina sobre a motilidade e concentração In Vitro dos espermatozóides humanos em indivíduos inférteis com oligoastenozoospermia e astenozoospermia

Manetta, Luiz Alberto

doi:10.1590/s0100-72031998000100011

Cited by 2 publications

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“…With the inhibition of the phosphodiesterase, there is an increase in the available AMPc levels, resulting in larger amount of active PK-A, which leads to a gain of sperm motility. 9 However, a question raised by Unsal et al 10 is the chance of DNA damage induced by pentoxifylline. The presence of fragmentation in the chromatin may have a negative impact on fertility rates, particularly in ICSI.…”

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confidence: 99%

Activation of the mobility of human spermatozoa with the use of pentoxifylline: effects on spermal DNA

Dutra¹,

Silva²,

Lazzari³

et al. 2017

Hum. Reprod. Arch.

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Introduction: 50% of pregnancy failure is due male factors. One of these factors is the absence of spermatozoa in the ejaculate; in these cases the patient is submitted to epididymal or testicular sperm aspiration. The spermatozoa acquired from these technics usually are immobile, what makes necessary the use of pentoxifylline, which activates sperm movement, to distinction of viable spermatozoa to ICSI. Objectives: To analyze if a low concentration of pentoxifylline causes spermatic chromatin damages. Materials and Methods: Sperm motility activation was tested in different concentrations of pentoxifylline. After the screening of doses, the lowest effective concentration was choose to analyze the chromatin damage rate. 15 samples of fresh sperm were tested for DNA damage with HALOSPERM  Kit. Blades' analyses were made in bright field microscopy. The fragmented DNA was settle by the absence of chromatin dispersion halo or small halo. Results: The lowest and effective pentoxifylline dose was 1.5 mM. There was no significant difference on chromatin damage rate between study and control groups (p = 0.55). Conclusion: The use of pentoxifylline at 1.5 mM does not affect the sperm DNA.

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Section: /7mentioning

confidence: 99%

Activation of the mobility of human spermatozoa with the use of pentoxifylline: effects on spermal DNA

Dutra¹,

Silva²,

Lazzari³

et al. 2017

Hum. Reprod. Arch.

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“…The selection of living spermatozoa for ICSI from frozen-thawed samples of NOA patients is very time consuming, difficult and sometimes impossible (Griveau et al, 2006). Pentoxifylline (PTX), a phosphodiesterase inhibitor, increases c-AMP levels and protein kinase A activity, and can increase sperm motility (Manetta, 1998). Nevertheless, a concern has been voiced by some investigators to the possible effects on the genome (Meseguer et al, 2009;Asokan et al, 2015) and clinical (Mahaldashtian et al, 2021) damage induced by PTX.…”

Section: Introductionmentioning

confidence: 99%

Pentoxifylline treatment had no detrimental effect on sperm DNA integrity and clinical characteristics in cases with non-obstructive azoospermia

Mahaldashtian

Khalili²,

Mangoli³

et al. 2022

Zygote

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Summary The aim of this study was to assess the consequences of treatment with pentoxifylline (PTX), an inducer of sperm motility, on sperm DNA fragmentation (SDF) and clinical characteristics in non-obstructive azoospermia (NOA) patients. The pilot study included 15 NOA patients. Half of each sperm sample before and after rapid freezing, was treated with PTX (3.6 mM /l, 30 min) as the PTX group and the remaining samples were considered as the control. SDF and sperm motility were assessed in each group. The clinical study comprised 30 fresh testicular sperm extractions (TESE) and 22 post-thawed TESE intracytoplasmic sperm injection cycles. Half of the mature oocytes from each patient were injected with PTX-treated spermatozoa and the remaining oocytes were injected with non-treated spermatozoa. Fertilization was assessed at 16 h post injection. Embryo transfer was carried out on day 2 after fertilization. Chemical pregnancy was assessed 2 weeks after transfer. PTX was found to significantly increase (P < 0.05) sperm motility. There was an insignificant difference in SDF rates between the groups (P > 0.05). In patient ovaries given fresh TESE, there was not any significant difference in clinical characteristics (P > 0.05). In patient ovaries given post-thawed TESE, there was a significant difference in the number of 2PN and in embryo formation (P < 0.05). Differences in the results of chemical pregnancy were insignificant (P > 0.05) between the groups. In addition, there was not any correlation between DNA fragmentation index and sperm motility and laboratory outcomes. Therefore, obtaining viable spermatozoa using PTX was more effective in post-thawed TESE regime patients in terms of 2PN and in embryo formation, deprived of damaging effects on sperm DNA integrity.

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Efeito da pentoxifilina sobre a motilidade e concentração In Vitro dos espermatozóides humanos em indivíduos inférteis com oligoastenozoospermia e astenozoospermia

Cited by 2 publications

References 0 publications

Activation of the mobility of human spermatozoa with the use of pentoxifylline: effects on spermal DNA

Activation of the mobility of human spermatozoa with the use of pentoxifylline: effects on spermal DNA

Pentoxifylline treatment had no detrimental effect on sperm DNA integrity and clinical characteristics in cases with non-obstructive azoospermia

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